Abstract
The cell growth, production of exopolymers, and the molar ratio of glucose to mannose in the water-soluble non curdlan type exopolymer-B (WSNCE-B), which is one of three exopolymers purified from the culture of Agrobacterium sp., varied with carbon source, culture medium, and initial medium pH. The molar percentage of rhamnose, a minor component in WSNCE-B, varied up to 13%, dependent on physiological conditions. No rhamnose was found in the WSNCE-B purified from the culture with initial medium pH > 6.8. The relative amount of mannose in WSNCE-B increased regularly with the amount of yeast extract added to the mineral salts medium. The relative amounts of glucose, mannose, and rhamnose in the WSNCE-B can be controlled by varying culture conditions.
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Aeschlimann, A. and von Stockar, U. 1990. Appl. Microbiol. Biotechnol. 32:398–402.
Armenante, P.M, Fava, F. and Kafkewitz, D. 1995. Biotechnol. Bioeng. 47:227–233.
Chaplin, M. 1982. Anal. Biochem. 123:336–341.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956). Anal. Chem. 28, 350–56.
Fava, F., Armenante, P.M., Kafkewitz, D., and Marchetti, L. 1995. Appl. Microbiol. Biotechnol. 43:171–177.
Harada, T., Masada, M., Fujimori, K., and Maeda, I. 1966a. Agr. Biol. Chem. 30:196–198.
Harada, T., Masada, M., Hidaka, H., and Takada, M. 1966b. J. Ferment. Technol. 44:20–24.
Harada, T., Fujimori, K., Hirose, S., and Masada, M. 1966c. Agri. Biol. Chem. 30:764–769.
Harada, T., Misaki, A., and Saito, H. 1968. Arch. Biochem. 124:292–298.
Hisamatsu, M., Amemura, A., Matsuo, T., Matsuda, H., and Harada, T. 1982. J. Gen. Microbiol. 128:1873–1879.
Kadowaki, S., Takegawa, K., Yamamoto, K., Kumagai, H., and Tochikura, T. 1988. Agri. Biol. Chem. 52:2105–2106.
Lawford, H. G., Philips, K. R., and Lawford, G. R. 1982. Biotechnol. Lett. 4:689–694.
Lee, J. W., Yeomans, W. G., Allen, A. L., Kaplan, D. L., Deng, F., and Gross, R. A. 1997. Can. J. Microbiol. 43:149–156.
Marty, N., Dournes, J., Chabanon, G., and Montrozier, H. 1992. FEMS Microbiol. Let. 98:35–44.
Phillips, K. R., Pik, J., Lawford, H. G., Lavers, B., Kligerman, A., and Lawford, G. R. 1983. Can. J. Microbiol. 29:1331–1338.
Sharmila, M., Ramanand, K., and Sethunathan, N. 1989. Can. J. Microbiol. 35:1105–1110.
Shen, C. F., Kosaric N., and Blaszczyk, R. 1993. Appl. Microbiol. Biotechnol. 39:132–137.
Slodki, M. E. 1987. New bacterial polysaccharides. In Industrial Polysaccharides. Edited by Stivala S. S. et al, Gordon and Breach Science Pub., New York. pp. 3–13
Sutherland, I. W. (Editor). 1990. Physiology and industrial production In Biotechnology of Microbial Exopolysaccharides. Cambridge Univ. Press, Cambridge. pp. 70–88.
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Lee, J., Yeomans, W., Allen, A. et al. Microbial production of water-soluble non curdlan type exopolymer-B with controlled composition by agrobacteriumsp. Biotechnology Letters 19, 1217–1221 (1997). https://doi.org/10.1023/A:1018442021914
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DOI: https://doi.org/10.1023/A:1018442021914