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Microbial production of water-soluble non curdlan type exopolymer-B with controlled composition by agrobacteriumsp

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Abstract

The cell growth, production of exopolymers, and the molar ratio of glucose to mannose in the water-soluble non curdlan type exopolymer-B (WSNCE-B), which is one of three exopolymers purified from the culture of Agrobacterium sp., varied with carbon source, culture medium, and initial medium pH. The molar percentage of rhamnose, a minor component in WSNCE-B, varied up to 13%, dependent on physiological conditions. No rhamnose was found in the WSNCE-B purified from the culture with initial medium pH > 6.8. The relative amount of mannose in WSNCE-B increased regularly with the amount of yeast extract added to the mineral salts medium. The relative amounts of glucose, mannose, and rhamnose in the WSNCE-B can be controlled by varying culture conditions.

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References

  • Aeschlimann, A. and von Stockar, U. 1990. Appl. Microbiol. Biotechnol. 32:398–402.

    Google Scholar 

  • Armenante, P.M, Fava, F. and Kafkewitz, D. 1995. Biotechnol. Bioeng. 47:227–233.

    Google Scholar 

  • Chaplin, M. 1982. Anal. Biochem. 123:336–341.

    Google Scholar 

  • Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956). Anal. Chem. 28, 350–56.

    Google Scholar 

  • Fava, F., Armenante, P.M., Kafkewitz, D., and Marchetti, L. 1995. Appl. Microbiol. Biotechnol. 43:171–177.

    Google Scholar 

  • Harada, T., Masada, M., Fujimori, K., and Maeda, I. 1966a. Agr. Biol. Chem. 30:196–198.

    Google Scholar 

  • Harada, T., Masada, M., Hidaka, H., and Takada, M. 1966b. J. Ferment. Technol. 44:20–24.

    Google Scholar 

  • Harada, T., Fujimori, K., Hirose, S., and Masada, M. 1966c. Agri. Biol. Chem. 30:764–769.

    Google Scholar 

  • Harada, T., Misaki, A., and Saito, H. 1968. Arch. Biochem. 124:292–298.

    Google Scholar 

  • Hisamatsu, M., Amemura, A., Matsuo, T., Matsuda, H., and Harada, T. 1982. J. Gen. Microbiol. 128:1873–1879.

    Google Scholar 

  • Kadowaki, S., Takegawa, K., Yamamoto, K., Kumagai, H., and Tochikura, T. 1988. Agri. Biol. Chem. 52:2105–2106.

    Google Scholar 

  • Lawford, H. G., Philips, K. R., and Lawford, G. R. 1982. Biotechnol. Lett. 4:689–694.

    Google Scholar 

  • Lee, J. W., Yeomans, W. G., Allen, A. L., Kaplan, D. L., Deng, F., and Gross, R. A. 1997. Can. J. Microbiol. 43:149–156.

    Google Scholar 

  • Marty, N., Dournes, J., Chabanon, G., and Montrozier, H. 1992. FEMS Microbiol. Let. 98:35–44.

    Google Scholar 

  • Phillips, K. R., Pik, J., Lawford, H. G., Lavers, B., Kligerman, A., and Lawford, G. R. 1983. Can. J. Microbiol. 29:1331–1338.

    Google Scholar 

  • Sharmila, M., Ramanand, K., and Sethunathan, N. 1989. Can. J. Microbiol. 35:1105–1110.

    Google Scholar 

  • Shen, C. F., Kosaric N., and Blaszczyk, R. 1993. Appl. Microbiol. Biotechnol. 39:132–137.

    Google Scholar 

  • Slodki, M. E. 1987. New bacterial polysaccharides. In Industrial Polysaccharides. Edited by Stivala S. S. et al, Gordon and Breach Science Pub., New York. pp. 3–13

    Google Scholar 

  • Sutherland, I. W. (Editor). 1990. Physiology and industrial production In Biotechnology of Microbial Exopolysaccharides. Cambridge Univ. Press, Cambridge. pp. 70–88.

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Lee, J., Yeomans, W., Allen, A. et al. Microbial production of water-soluble non curdlan type exopolymer-B with controlled composition by agrobacteriumsp. Biotechnology Letters 19, 1217–1221 (1997). https://doi.org/10.1023/A:1018442021914

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