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A laser-diffraction capillary assay to measure random motility in bacteria

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Biotechnology Techniques

Abstract

A laser-diffraction capillary assay was developed to measure bacterial random motility coefficients in semi-solid media. A capillary was filled successively with two agar suspensions, one of which contained bacteria. A laser was directed axially through the capillary. Random motility coefficients were calculated for Pseudomonas stutzeri KC and Escherichia coli at different agar concentrations.

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Authors and Affiliations

  1. Department of Chemical Engineering, Michigan State University, East Lansing, Michigan, 48824, USA

    Sebastian Schmidt, Mark T. Widman & R. Mark Worden

  2. Forschungszentrum Jülich GmbH, Institut für Biotechnologie 2, 52425, Jülich, Germany

    Sebastian Schmidt

Authors
  1. Sebastian Schmidt
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  2. Mark T. Widman
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  3. R. Mark Worden
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Schmidt, S., Widman, M.T. & Mark Worden, R. A laser-diffraction capillary assay to measure random motility in bacteria. Biotechnology Techniques 11, 423–426 (1997). https://doi.org/10.1023/A:1018425008111

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  • DOI: https://doi.org/10.1023/A:1018425008111

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