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A comparison of gel filtration chromatographic supports for plasmid purification

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Biotechnology Techniques

Abstract

Two gel filtration chromatographic supports, Superose 6 and Sephacryl S1000 SF, were used to purify supercoiled plasmids using a 4.8 kb plasmid as a model. Both supports purified the plasmid from RNA and other small molecular weight contaminants, as shown by agarose electrophoresis and ion exchange HPLC, with an overall yield of 70%. Sephacryl S1000 SF was the better support as it resolved supercoiled, relaxed, linear and concatamer plasmid forms, and chromosomal DNA.

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Authors and Affiliations

  1. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1096, Lisboa Codex, Portugal

    G.N.M. Ferreira, J.M.S. Cabral & D.M.F. Prazeres

Authors
  1. G.N.M. Ferreira
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  2. J.M.S. Cabral
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  3. D.M.F. Prazeres
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Ferreira, G., Cabral, J. & Prazeres, D. A comparison of gel filtration chromatographic supports for plasmid purification. Biotechnology Techniques 11, 417–420 (1997). https://doi.org/10.1023/A:1018420807203

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  • DOI: https://doi.org/10.1023/A:1018420807203

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