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Simulation of a reactor for glucose isomerization to fructose by immobilized glucose isomerase with continuous enzyme renewal

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Biotechnology Techniques

Abstract

Two different dispositions of laboratory-scaled columns have been tested to simulate the isomerization of glucose to fructose in a mobile bed reactor where exhausted immobilized glucose isomerase is continuously renewed. If the simulation columns working at 65°C are arranged in parallel and connected to a section for final enzyme exploitation at 75°C, a syrup with constant composition can be produced, at relatively constant total throughput, by feeding the individual columns at flow rate decreasing according to the enzyme decay profile and following a programmed disphased mode of operation.

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References

  • Chen, K.-C., Chang, C.M. 1984. Operational stability of immobilized D-glucose isomerase in a continuous feed stirred tank reactor. Enzyme Microb. Technol. 6: 359-364.

    Article  Google Scholar 

  • Chen, K.-C., Wu, J.-Y. 1987. Substrate protection of immobilized glucose isomerase. Biotechnol. Bioeng. 30: 817-824.

    Article  Google Scholar 

  • Converti, A., Del Borghi, M. 1996.a. Thermodynamic study of glucose isomerization to fructose by immobilized glucose isomerase [in Italian]. Proc. 15th Scientific Congress of the Italian Society of General Microbiology and Microbial Biotechnology. Abbadia San Salvatore, SI, 8–11 October 1996, Poster session n. 4.

  • Converti, A., Del Borghi, M. 1996.b. Simultaneous effects of immobilization and substrate protection on the thermodynamics of glucose isomerase activity and inactivation. Enzyme Microb. Technol. Submitted.

  • Economic European Community 1981. Regulament n. 1785/81. Bruxelles, 30 June 1981.

  • Houng, J.-Y., Yu, H.-J., Chen, K.-C. 1993. Analysis of substrate protection of an immobilized glucose isomerase reactor. Biotechnol. Bioeng. 41: 451-458.

    Article  Google Scholar 

  • Illanes, A., Zuñiga, M.E., Contreras, S., Guerrero, A. 1992. Reactor design for the enzymatic isomerization of glucose to fructose. Bioproc. Eng. 7: 199-204.

    Article  Google Scholar 

  • Rechigl, M. 1982. Handbook of nutritive value of processed foods. CRC Press, Boca Raton, pp. 80-81.

    Google Scholar 

  • Riscolo, A., Fisichella, A. 1996. Optimization of the continuous process of glucose isomerization to fructose by immobilized isomerase [in Italian]. Thesis and Dissertation. Genoa University, Italy.

    Google Scholar 

  • Roels, J.A. 1983. Energetics in biotechnology. Elsevier, Amsterdam.

    Google Scholar 

  • van den Tweel, W.J., Harder, A., Buitelaar, R.M. 1993. Stability and stabilization of enzymes. Elsevier, Amsterdam.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Chemical and Process Engineering, University of Genoa, Via Opera Pia, 15, 16145, Genoa, Italy

    A. Converti, A. Fisichella, A. Riscolo & M. Del Borghi

  2. Roquette Italia SpA, Via Serravalle, 26, 15063, Cassano Spinola, AL, Italy

    A. Scaringi & D. Carbone

Authors
  1. A. Converti
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  2. A. Fisichella
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  3. A. Riscolo
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  4. M. Del Borghi
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  5. A. Scaringi
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  6. D. Carbone
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Converti, A., Fisichella, A., Riscolo, A. et al. Simulation of a reactor for glucose isomerization to fructose by immobilized glucose isomerase with continuous enzyme renewal. Biotechnology Techniques 11, 99–103 (1997). https://doi.org/10.1023/A:1018420623190

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  • DOI: https://doi.org/10.1023/A:1018420623190

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