Kinetic study of enantioselective esterification of ketoprofen with n-propanol catalysed by an lipase in an organic medium

Abstract

A kinetic study of an immobilised lipase esterification reaction in dipropyl ether for resolution of ketoprofen indicated a Bi Bi Ping Pong mechanism with dead-end inhibition of the alcohol was occurring for both enantiomers and this is was confirmed experimentally. Parameters in the kinetic equation and reaction activation energies for the two enantiomers were determined by non-linear regression.

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References

  1. Bailey, JE and Ollis, DF(1986). Biochemical Engineering Fundamentals, 2nd ed, 212-214, Singapore: McGraw-Hill International Editions

    Google Scholar 

  2. Cabral, JMS, Best, D, Boross, L and Tramper, J (1994). Applied Biocatalysis. Switzerland: Harwood Academic Publishers

    Google Scholar 

  3. Chen, CS and Sih, CJ (1989). Angew Chem Int Ed Engl 28: 695-707

    Google Scholar 

  4. Duan, G and Chen, JY (1994). Biotechnol Lett 16: 1065-1068

    Google Scholar 

  5. Famaey, JP and Paulus, HE (1992). Therapeutic Applications of SAIDs. New York: Marcel Dekker

    Google Scholar 

  6. Klibanov, AM (1989) Trends Biochem Sci 14: 141-144

    Google Scholar 

  7. Perry, RH, Green, DW and Maloney, JO (1984). Perry's Chemical Engineer's Handbook, 6th ed, Singapore: McGraw-Hill International Edition

    Google Scholar 

  8. Segal, IH (1975). Enzyme Kinetics, New York: John Wiley and Sons.

    Google Scholar 

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Duan, G., Ching, C., Lim, E. et al. Kinetic study of enantioselective esterification of ketoprofen with n-propanol catalysed by an lipase in an organic medium. Biotechnology Letters 19, 1051–1055 (1997). https://doi.org/10.1023/A:1018420022398

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Keywords

  • Alcohol
  • Ether
  • Activation Energy
  • Organic Chemistry
  • Lipase