Abstract
A kinetic study of an immobilised lipase esterification reaction in dipropyl ether for resolution of ketoprofen indicated a Bi Bi Ping Pong mechanism with dead-end inhibition of the alcohol was occurring for both enantiomers and this is was confirmed experimentally. Parameters in the kinetic equation and reaction activation energies for the two enantiomers were determined by non-linear regression.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, JE and Ollis, DF(1986). Biochemical Engineering Fundamentals, 2nd ed, 212-214, Singapore: McGraw-Hill International Editions
Cabral, JMS, Best, D, Boross, L and Tramper, J (1994). Applied Biocatalysis. Switzerland: Harwood Academic Publishers
Chen, CS and Sih, CJ (1989). Angew Chem Int Ed Engl 28: 695-707
Duan, G and Chen, JY (1994). Biotechnol Lett 16: 1065-1068
Famaey, JP and Paulus, HE (1992). Therapeutic Applications of SAIDs. New York: Marcel Dekker
Klibanov, AM (1989) Trends Biochem Sci 14: 141-144
Perry, RH, Green, DW and Maloney, JO (1984). Perry's Chemical Engineer's Handbook, 6th ed, Singapore: McGraw-Hill International Edition
Segal, IH (1975). Enzyme Kinetics, New York: John Wiley and Sons.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Duan, G., Ching, C., Lim, E. et al. Kinetic study of enantioselective esterification of ketoprofen with n-propanol catalysed by an lipase in an organic medium. Biotechnology Letters 19, 1051–1055 (1997). https://doi.org/10.1023/A:1018420022398
Issue Date:
DOI: https://doi.org/10.1023/A:1018420022398