Skip to main content
SpringerLink
Log in

Recovery of poly-3-hydroxybutyrate from recombinant Escherichia coli by homogenization and centrifugation

  • Published:
Biotechnology Techniques

Abstract

Poly-3-hydroxybutyrate from recombinant E. coli was recovered using homogenization and continuous centrifugation with a purity of 94%. Final protein and DNA concentrations were 1.0% w/w and 1.9% w/w, respectively, when a hypochlorite treatment was employed prior to centrifugation. High fractional cell debris removal (94%) was achieved with two centrifugation steps.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Berger, E., Ramsay, B.A., Ramsay, J.A. and Chavarie, C. (1989). Biotechnol. Tech. 3, 227–232.

    Google Scholar 

  • Braunegg, G., Sonnleitner, B. and Lafferty, R.M. (1978). Euro. J. Appl. Microbiol. Biotechnol. 6, 29–37.

    Google Scholar 

  • Burton, K. (1968). Meth. Enzymol. 12B. 163–166.

    Google Scholar 

  • Hahn, S.K., Chang, Y.K. and Lee, S.Y. (1995). Appl. Environ. Microbiol. 61, 34–39.

    Google Scholar 

  • Harrison, S.T. (1990). PhD thesis, University of Cambridge, U.K.

  • Hatt, J., Callaham, M. and Greener, A. (1992). Stratagies 5, 2–3.

    Google Scholar 

  • Lee, S.Y. and Chang, H.N. (1995). Can. J. Microbiol. 41, 207–215.

    Google Scholar 

  • Middelberg, A.P.J. (1996). J. Microbiol. Biotechnol. 6, 225–231.

    Google Scholar 

  • Middelberg, A.P.J., Bogle, I.D.L. and Snoswell, M.A. (1990). Biotechnol. Prog. 6, 255–261.

    Google Scholar 

  • Middelberg, A.P.J., Lee, S.Y., Martin, J., Williams, D.R.G. and Chang, H.N. (1995). Biotechnol. Lett. 17, 205–210.

    Google Scholar 

  • Slater, S.C., Gallaher, T. and Dennis, D.E. (1992). Appl. Environ. Microbiol. 58, 1089–1094.

    Google Scholar 

  • Wong, H.H., O'Neill, B.K. and Middelberg, A.P.J. (1996). Bioseparation 6, 185–192.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Adelaide, S.A, 5005, Australia

    Y. Ling, D.R.G. Williams & A.P.J. Middelberg

  2. Department of Microbiology and Immunology, University of Adelaide, S.A, 5005, Australia

    C.J. Thomas

Authors
  1. Y. Ling
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D.R.G. Williams
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C.J. Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A.P.J. Middelberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ling, Y., Williams, D., Thomas, C. et al. Recovery of poly-3-hydroxybutyrate from recombinant Escherichia coli by homogenization and centrifugation. Biotechnology Techniques 11, 409–412 (1997). https://doi.org/10.1023/A:1018416706294

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018416706294

Keywords

Search

Navigation