Abstract
High-resolution bivariate flow karyotypes were obtained using fibroblast cell lines from a sheep with a normal karyotype (2n=54), from sheep carrying Robertsonian translocation chromosomes and from sheep—hamster somatic cell hybrids. By taking advantage of the presence of chromosome polymorphisms, translocation chromosomesand sheep—hamster somatic cell hybrids, all sheep chromosomes were isolated by flow sorting. Chromosome-specific paints were generated from each sorted peak using degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). The sheep chromosome present in each peak was identified by chromosome-specific microsatellite analysis of the DOP-PCR products and fluorescence in situ hybridization (FISH) onto DAPI-banded sheep metaphase chromosomes. The chromosome-specific DNA obtained in this study can be used for the production of genomic libraries and as a resource for mapping randomly cloned DNA sequences that will greatly aid the construction of genetic and physical maps in the sheep. The chromosome-specific paints will facilitate chromosome identification and contribute to the study of karyotype evolution in the sheep and related species.
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Burkin, D.J., O'Brien, P.C., Broad, T.E. et al. Isolation of chromosome-specific paints from high-resolution flow karyotypes of the sheep (Ovis aries). Chromosome Res 5, 102–108 (1997). https://doi.org/10.1023/A:1018414123751
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DOI: https://doi.org/10.1023/A:1018414123751