Abstract
A surfactant-horseradish peroxidase complex was prepared with a nonionic surfactant by utilizing water-in-oil emulsions. The complex was readily soluble in anhydrous benzene in which it proved to be an excellent biocatalyst for the oxidation of o-phenylenediamine using H 2 O 2 or tert-butyl hydroperoxide as oxidants.
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Blinkovsky, A.M., McEldoon, J.P., Arnold, J.M., Dordick, J.S. (1994) Appl. Biochem. Biotechnol., 49, 153–164.
Dordick, J.S., Marletta, M.A., Klibanov, A.M. (1987) Biotechnol. Bioeng., 30, 31–36.
Goto, M., Kamiya, N., Miyata, M., Nakashio, F. (1994) Biotechnol. Prog., 10, 263–268.
Kamiya, N., Murakami, E., Goto, M., Nakashio, F. (1996) J. Ferment. Bioeng., 82, 37–41.
Mabrouk, P.A. (1995) J. Am. Chem. Soc., 117, 2141–2146.
Meyer, J.D., Kendrick, B.S., Matsuura, J.E., Ruth, J.A., Bryan, P.N., Manning, M.C. (1996) Int. J. Peptide Protein Res., 47, 177–181.
Okazaki, S., Kamiya, N., Goto, M., Nakashio, F. (1997) Biotechnol. Bioeng., in press.
Takahashi, K., Nishimura, H., Yoshimoto, T., Saito, Y., Inada, Y. (1984) Biochem. Biophys. Res. Commun., 121, 261–265.
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Kamiya, N., Okazaki, Sy. & Goto, M. Surfactant-horseradish peroxidase complex catalytically active in anhydrous benzene. Biotechnology Techniques 11, 375–378 (1997). https://doi.org/10.1023/A:1018400302660
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DOI: https://doi.org/10.1023/A:1018400302660