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Characterisation of European oat germ plasm: allelic variation at complex avenin loci detected by acid polyacrylamide gel electrophoresis

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Abstract

European oat germ plasm involving 252 varieties, landraces, and breeding lines has been characterised by allelic constitution at three independent complex avenin loci. A total of 26 avenin alleles (blocks), including 7 at the Ave1 (Avn A), 10 at the Ave2 (Avn B), and 9 at the Ave3 (Avn C) loci were revealed. The avenin block variants were shown to be coded by gene clusters of 2–5 single expressed genes. A catalogue of the identified avenin blocks as well as a list of the avenin phenotypes is presented. It has been discovered that about 8% of the oat varieties were heterogeneous comprising two or three different avenin profiles. The varieties assayed have been classified into 79 avenin phenotypes made up by the different alleles. Forty-six of these phenotypes were distinguished uniquely, while the other 33 were shared by groups possessing from 2 to 41 genotypes each. Members within these groups had certain ancestors in common. Non-random patterns of both the avenin allele and phenotype distributions have been found.

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References

  • Baum, B.R., 1972. Material for an International Oak Register. Ottawa.

  • Chong, J., N.K. Howes, P.D. Brown & D.E. Harder, 1994. Identification of the stem rust resistance gene Pg9and its association with crown rust resistance and endosperm proteins in 'Dumont' oat. Genome 37: 440-447.

    CAS  Google Scholar 

  • Ciaffi, M., L. Dominici & D. Lafiandra, 1997. Gliadin polymorphism in wild and cultivated einkorn wheats. Theor Appl Genet 94: 68- 74.

    Article  CAS  Google Scholar 

  • Cliff, E.M. & R.J. Cooke, 1984. The characterisation of oat cultivars by electrophoresis. J Natl Inst Agric Bot 16: 415-429.

    Google Scholar 

  • Coe, E., 1993. Gene list and working maps. Maize Genet Newslet 67: 133-166.

    Google Scholar 

  • Coffman, F.A., 1977. Oat history, identification & classification. Technical Bull N 1516. USDAARS, Washington DC.

  • Cooke, R.J., 1995. Allelic variability at the Glu-1loci in wheat varieties. Plant Var Seeds 8: 97-106.

    Google Scholar 

  • Kim, S.I., L. Charbonnier & J. Mosse, 1978. Heterogeneity of avenin, the oat prolamin. Fractionation, molecular weight and amino acid composition. Biochem Biophys Acta 537: 22-30.

    PubMed  CAS  Google Scholar 

  • Kim, S.I., L. Saur & J. Mosse, 1979. Some features of the inheritance of avenins, the alcohol soluble protein of oat. Theo Appl Genet 54: 49-54.

    Article  CAS  Google Scholar 

  • Kreis, M., P.R. Shewry, B.G. Forde, J. Forde & B.J. Mflin, 1985. Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxford Surv Plant Mol Cell Biol 2: 253-317.

    CAS  Google Scholar 

  • Lagudah, E.S. & G.M. Halloran, 1988. Phylogenetic relationships of Triticum tauchii, the Dgenome donor to hexaploid wheat. 1. Variation in HMW subunits of glutenin and gliadins. Theor Appl Genet 75: 592-598.

    Article  CAS  Google Scholar 

  • Linde-Laurens, I., H. Doll & G. Nielsen, 1982. Giemsa Cbanding patterns and some biochemical markers in a pedigree of European barley. Pflanzenzüchtung 88: 191-219.

    Google Scholar 

  • Meltz, G., R. Schlegel & V. Thiele, 1992. Genetic linkage map of rye (Secale cerealeL.). Theor Appl Genet 85: 33-45.

    Google Scholar 

  • Metakovsky, E.V., A. Yu. Novoselskaya, M.M. Kopus, T.A. Sobko & A.A. Sozinov, 1984. Blocks of gliadin components in winter wheat detected by one dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 67: 559-568.

    Article  Google Scholar 

  • Metakovsky, E.V., A.M. Kudryavtsev, Z.A. Iakobashvili & A. Yu. Novoselskaya, 1989. Analysis of phylogenetic relations of durum, carthlicum and common wheats by means of comparison of alleles of gliadin coding loci. Theor Appl Genet 77: 881-887.

    Article  Google Scholar 

  • Metakovsky, E.V., C.V. Wrigley, F. Bekes & R.B. Gupta, 1990. Gluten polypeptides as useful genetic markers of dough quality in Australian wheats. Aust J Agric Res 41: 289-306.

    Article  Google Scholar 

  • Metakovsky, E.V., D. Knezevic & B. Javornik, 1991. Gliadin allele composition of Yugoslav winter wheat cultivars. Euphytica 54: 285-295.

    Google Scholar 

  • Milne, D.L. & R.A. McIntosh, 1990. Triticum aestivum(common wheat). In: S.J. O'Brien (Ed.), Genetic Maps, pp. 6.16-6.27. Cold Spring Harbor, N.Y.

    Google Scholar 

  • Mordvinkina, A.I., 1947. Oat. General section. In: A guide for cultivar identification in crops. Small grains (wheat, rye, barley, oat), pp. 535-548 (in Russian). OGIZ-Selkhozgiz Publishers, Moscow.

    Google Scholar 

  • Mordvinkina, A.I. & N.N. Kudryavtseva, 1947. Oat cultivars. In: A guide for cultivar identification in crops. Small grains (wheat, rye, barley, oat), pp. 548-579 (in Russian). OGIZ-Selkhozgiz Publishers, Moscow.

    Google Scholar 

  • Mordvinkina, A.I. & N.A. Rodionova, 1976. Oat. In: M.G. Prutskova (Ed.), A guide for cultivar identification in crops, pp. 174-202 (in Russian). Kolos Publishers, Moscow.

    Google Scholar 

  • Nevo, E., A. Beiles, N. Storch, H. Doll & B. Andersen, 1983. Microgeographic edaphic differentiation in hordein polymorphism of wild barley. Theor Appl Genet 68: 123-132.

    Article  Google Scholar 

  • O'Donoughue, L.S., S.F. Kianian, P.J. Rayapati, G.A. Penner, M.E. Sorrells, S.D. Tanksley, R.L. Phillips, H.W. Rines, M. Lee, G. Fedak, S.J. Molnar, D. Hoffman, C.A. Salas, B. Wu, E. Autrique & A. van Deynze, 1995. A molecular linkage map of cultivated oat. Genome 38: 368-380.

    Google Scholar 

  • O'Donoughue, L.S., J. Chong, C.P. Wight, G. Fedak & J. Molnar, 1996. Localization of stem rust resistance genes and associated molecular markers in cultivated oat. Phytopathology 86: 719- 727.

    Google Scholar 

  • Payne, P.I., L.M. Holt, G.J. Lawrence & C.N. Law, 1982. The genetics of gliadin and glutenin, the major storage proteins of the wheat endosperm. Qual Plant Foods Hum Nutr 31: 229-241.

    Article  CAS  Google Scholar 

  • Portyanko, V.A., A.A. Pomortsev & A.A. Sozinov, 1987a. Intravarietal and intervarietal polymorphism of oat prolamins. Sov Agric Sci 1: 12-15 (Engl. Transl. Doklady VASKhNIL, Moscow).

    Google Scholar 

  • Portyanko, V.A., A.A. Pomortsev, N.A. Kalashnik, V.I. Bogatchkov & A.A. Sozinov, 1987b. Genetic control of avenins and the principles of their classification. Sov Genet 23: 584-590 (Engl Transl Genetica, Moscow).

    Google Scholar 

  • Portyanko, V.A., N.R. Sharopova & A.A. Sozinov, 1995a. Avenin polymorphism and its application to analysis of divergence among oat varieties in world breeding. Russian J Genet 31: 1099- 1104 (Engl Transl Genetica, Moscow).

    CAS  Google Scholar 

  • Portyanko, V.A., N.R. Sharopova & A.A. Sozinov, 1995b. Associated variation of avenin loci and quantitative traits determining oat productivity. Cytol Genet 29: 45-50 (Engl Transl Tsitologia i Genetika, Kiev).

    Google Scholar 

  • Rayapati, P.J., V.A. Portyanko & M. Lee, 1994. Placement of loci for avenins and resistance to Puccinia coronatato a common linkage group in Avena strigosa. Genome 37: 900-903.

    CAS  Google Scholar 

  • Sharopova, N.R., V.A. Portyanko & A.A. Sozinov, 1997. Identification of cultivars and lines in oat using protein markers. Tsitologia i Genetika, Kiev 31: 23-28 (Russian with English summary).

    Google Scholar 

  • Shewry, P.R., D. Bradberry, J. Franklin & R.P. White, 1984. The chromosomal locations and linkage relationships of the structural genes for the prolamine storage proteins (secalins) of rye. Theor Appl Genet 69: 73-69.

    Article  Google Scholar 

  • Shewry, P.R., A.S. Tatham, J.M. Field, B.G. Forde, J. Clark, P. Gallois, C. Marris, N. Halford, J. Forde & M. Kreis, 1988. The structures of barley and wheat prolamines and their genes. Biochem Physiol Pflanz 183: 178-127.

    Google Scholar 

  • Shotwell, M.A., S.K. Boyer, R.S. Chesnut & B.A. Larkins, 1990. Analysis of seed storage protein genes of oats. J. Biol Chem 265: 9652-9658.

    PubMed  CAS  Google Scholar 

  • Souza, E. & M. Sorrells, 1990. Inheritance and distribution of variation at four avenin loci in American oat germ plasm. Genome 33: 416-424.

    Google Scholar 

  • Sozinov, A.A. & F.A. Poperelya, 1980. Genetic classification of prolamines and its use for plant breeding. Ann Technol Agric 29: 229-245.

    CAS  Google Scholar 

  • Sozinov, A.A., A.A. Pomortsev & B.P. Netsvetaev, 1987. Blocks of hordein components as genetic markers. In: S. Yasuda & T. Konishi (Eds), Barley Genetics V, pp. 887-894. Proc 5th Int Barley Genet Symp, Okayama.

  • Stanton, T.R., 1936. Superior germ plasm in oats. In: Yearbook of agriculture, pp. 347-414. USDA, Washington D.C.

    Google Scholar 

  • Stanton, T.R., 1955. Oat identification and classification. USDA, Washington D.C.

    Google Scholar 

  • von Wettstein-Knowles, P., 1990. Barley (Hordeum vulgare). In: S.J. O'Brien (Ed.), Genetic Maps, pp. 6.125-6.134. Cold Spring Harbor, N.Y.

    Google Scholar 

  • Wrigley, C.A., J.C. Autran & W. Bushuk, 1982. Identification of cereal varieties by gel electrophoresis of the grain proteins. Adv Cereal Sci Technol 5: 211-259.

    CAS  Google Scholar 

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Author notes

  1. V.A. Portyanko & N.R. Sharopova

    Present address: Department of Agronomy, Iowa State University, Ames, 1A, 50011, U.S.A

Authors and Affiliations

  1. Ukrainian Academy of Agricultural Sciences, Institute of Agroecology & Biotechnology, Metrologytcheskaya St. # 12, Kiev, 252143, Ukraine

    V.A. Portyanko, N.R. Sharopova & A.A. Sozinov

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  1. V.A. Portyanko
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  2. N.R. Sharopova
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  3. A.A. Sozinov
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Portyanko, V., Sharopova, N. & Sozinov, A. Characterisation of European oat germ plasm: allelic variation at complex avenin loci detected by acid polyacrylamide gel electrophoresis. Euphytica 102, 15–27 (1998). https://doi.org/10.1023/A:1018399919953

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