Abstract
D-Carnitine was converted to L-carnitine by resting and permeabilized cells as well as with purified stereospecific carnitine dehydrogenases from Agrobacterium sp. With permeabilized cells only 11% of D-carnitine was converted into L-carnitine. Using highly stereospecific D- and L-carnitine dehydrogenases from Agrobacterium sp. (pH 8.5, 50 mM D-carnitine, 1 mM NAD + , 0.1 mM NADH, 25-fold excess of L-carnitine dehydrogenase) almost 50% of the D-carnitine could be converted into L-carnitine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Achenbach, H., Kühn, H.-J., Lössner, J. and Seim, H. (1985). Wiss. Z. KMU Leipzig, Math.-Nat. R. 34, 259–272.
Bergmeyer, H.U. (1985). In: Methods of Enzymatic Analysis, Vol. 8, 3rd ed., pp. 481–487, VCH Weinheim.
Bounoure, J. and Souppe, J. (1988). Analyst 113, 1143–1144.
Bremer, J. (1963). J. Biol. Chem. 238, 2774–2779.
Cavazza, C. (1980). Germ. Offen. DE 2927672.
Cederbaum, S.D., Auestad, N. and Bernar, J. (1984). New Engl. J. Med. 310, 1395–1396.
Eichler, K., Bourgis, A., Buchet, A., Kleber, H.-P. and Mandrand-Berthelot, M.A. (1994). Mol. Microbiol. 13, 775–786.
Fritz, J.B. and Yue, K.T.N. (1963). J. Lipid Res. 4, 279–288.
Gilbert, E.F. (1985). Pathology 17, 161–169.
Hanschmann, H., Doß, A. and Kleber, H.-P. (1994). FEMS Microbiol. Lett. 119, 371–376.
Hanschmann, H., Ehricht, R. and Kleber, H.-P. (1996). Biophys. Biochim. Acta 1290, 177–183.
Hanschmann, H. and Kleber, H.-P. (1997). Biophys. Biochim. Acta 1337, 133–142.
Jung, H., Jung, K. and Kleber, H.-P. (1993). Adv. Biochem. Engin. Biotechnol. 50, 21–44.
Jung, H. and Kleber, H.-P. (1991). Appl. Microbiol. Biotechnol. 35, 393–395.
Meier, P.J. (1987), D-Carnitin harmlos? In: Carnitin in der Medizin, R. Gitzelmann, K. Baerlocher und B. Steinmann, eds. pp. 114–124, Schattauer Stuttgart-New York.
Miller, J.H. (1972). Experiments in Molecular Genetics. Cold Spring Habour Laboratory, Cold Spring Habour, New York, pp. 431–432.
Müller, D.M. and Strack, E. (1972). Hoppe-Seyler's Z. Physiol. Chem. 353, 618–622.
Rebouche, C.J. and Paulson, D.J. (1986). Annu. Rev. Nutr. 6, 41–66.
Schärer, K. (1987). Carnitinverlust durch Hämodialyse. In: Carnitin in der Medizin, R. Gitzelmann, K. Baerlocher und B. Steinmann, eds. pp. 114–124, Schattauer Stuttgart-New York.
Sih, C. (1984). D-OS 3344085 A1.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hanschmann, H., Kleber, HP. Conversion of D-carnitine into L-carnitine with stereospecific carnitine dehydrogenases. Biotechnology Letters 19, 679–682 (1997). https://doi.org/10.1023/A:1018399001074
Issue Date:
DOI: https://doi.org/10.1023/A:1018399001074