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Apoptosis extent increases in hybridoma cultures with temperature

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Abstract

Apoptosis plays an important role in determining efficacies of bioreactors employing hybridoma cells. Exposure to a 42°C shock for 1 h increased the apoptosis extent (DNA fragmentation) by 32% in CC9C10 hybridoma. Further, glutamine at 2 mM decreased temperature-induced apoptosis by about 20%.

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Authors and Affiliations

  1. Cell Science, National Centre, Ganeshkind Road, Pune, India

    Nagarathnamma

  2. Biochemical Engineering Group, Dept. of Chemical Engineering, Indian Institute of Technology, Bombay, Powai Mumbai, India

    M.M. Rajan & G.K. Sureshkumar

Authors
  1. Nagarathnamma
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  2. M.M. Rajan
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  3. G.K. Sureshkumar
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Nagarathnamma, Rajan, M. & Sureshkumar, G. Apoptosis extent increases in hybridoma cultures with temperature. Biotechnology Letters 19, 669–673 (1997). https://doi.org/10.1023/A:1018394900166

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  • DOI: https://doi.org/10.1023/A:1018394900166

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