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Transesterification of alkylphosphate esters by phospolipase D

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Abstract

Phospholipase D from cabbage or Streptomyces chromofuscus catalyses the transesterification of alkylphosphate esters, which are potent antitumor agents. The exchange of choline in octadecylphosphosphocholine by N-(2-hydroxy-ethyl)-N-methylpiperidine with phospholipase D from cabbage is kinetically controlled and amounts to maximum 60% in buffer, 43% in buffer/n-hexane, and 82% in buffer/n-hexane/1-octanol.

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Authors and Affiliations

  1. Department of Biochemistry/Biotechnology, Martin-Luther University, Halle-Wittenberg, D-6099, Halle, Federal Republic of Germany

    I. Aurich, P. Dürrschmidt, F. Hirche & R. Ulbrich-Hofmann

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  1. I. Aurich
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  2. P. Dürrschmidt
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  3. F. Hirche
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  4. R. Ulbrich-Hofmann
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Aurich, I., Dürrschmidt, P., Hirche, F. et al. Transesterification of alkylphosphate esters by phospolipase D. Biotechnology Letters 19, 875–879 (1997). https://doi.org/10.1023/A:1018389621064

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  • DOI: https://doi.org/10.1023/A:1018389621064

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