Abstract
Surfactin, an acidic lipopeptide of Bacillus subtilis, at 70 μM non-competitively inhibited alkaline phosphatase without its glycosyl-phosphatidylinositol anchor by 50%. Enzyme activity was restored by adding 50 μM ZnCl 2 and 1 mM MgCl2 confirming the chelating action of the free carboxyl groups of the Asp and Glu residues in the peptide moiety of surfactin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Angrand M. (1997) Thesis, no 030-97, UCB, Lyon I, France.
Arima K., Kakinuma A. and Tamura G. (1968) Biophys. Res. Commun. 31, 488–494.
Bernheimer A.W. and Avigad L.S. (1970) J. Gen. Microbiol. 61, 361–369.
Besson F., Hourdou M.L. and Michel G. (1990) Biochim. Biophys. Acta 1036, 101–106.
Besson F., Tenoux I., Hourdou M.L. and Michel G. (1992) Biochim. Biophys. Acta 1123, 51–58.
Coleman J.E. (1992) Annu. Rev. Biophys. Biomol. Struct. 21, 441–483.
Cyboron G.W. and Wuthier R.E. (1981) J. Biol. Chem. 256, 7262–7268.
Dixon M. and Webb C. (1964) Enzymes, 2nd edn, Longmans Green, London 327–331.
Holmquist B. (1988) Methods in Enzymol. 158, 6–12.
Hosono K. and Suzuki H. (1983a) J. Antibiotics 36, 667–673.
Hosono K. and Suzuki H. (1983b) J. Antibiotics 36, 673–678.
Hosono K. and Suzuki H. (1983c) J. Antibiotics 36, 679–683.
Itokawa H., Miyashita T., Morita H., Takeya K., Hirano T., Homma M. and Oka K. (1994) Chem. Pharm. Bull. 42, 604–607.
Kameda Y., Ouhira S., Matsui K., Kanatomoto S., Hase T. and Atsusaka T. (1974) Chem. Pharm. Bull. 22, 938–944.
Kim E.E. and Wyckoff H.W. (1991) J. Mol. Biol. 218, 449–464.
Low M.G. (1989) Biochem. Biophys. Acta, 988, 427–454.
Radisson J., Angrand M., Chavassieux P., Roux B. and Azzar G. (1996) Int. J. Biochem. Cell. Biol. 28, 421–430.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bortolato, M., Besson, F. & Roux, B. Inhibition of alkaline phosphatase by surfactin, a natural chelating lipopeptide from Bacillus subtilis. Biotechnology Letters 19, 433–435 (1997). https://doi.org/10.1023/A:1018387909310
Issue Date:
DOI: https://doi.org/10.1023/A:1018387909310