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Genetics of self-compatibility in a self-incompatible wild diploid potato species Solanum chacoense. 2. Localization of an S locus inhibitor (Sli) gene on the potato genome using DNA markers

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Abstract

A self-compatible (SC) hybrid plant F1-1 was obtained from a cross between a SC variant of a wild diploid potato species, Solanum chacoense, and a self-incompatible (SI) cultivated diploid species, S. phureja. The clone F1-1 has previously been proposed to have a dominant S locus inhibitor gene (Sli) in a heterozygous condition. It was crossed as a male parent with a selected clone from a S. stenotomum-S. phureja population, resulting in a segregating population consisting of 116 hybrid plants. Self-compatibility was assessed by selfing each of the hybrids. Sixty-six of them were SC, while 35 were SI, showing a significant distortion from an expected Mendelian ratio of 1:1. A genetic linkage map was constructed using DNA markers to localize the Sli gene. A total of 28 RAPD and 127 RFLP markers identified 109 mapping positions on 12 linkage groups. The Sli gene was mapped at a distal end of chromosome 12. Since the S locus has been localized on chromosome 1 on the potato RFLP map, it is confirmed that the Sli gene is independent of the S locus.

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Authors and Affiliations

  1. Experimental Farm, Kobe University, 1348 Uzurano, Kasai, Hyogo, 675-2103, Japan

    Kazuyoshi Hosaka

  2. USDA, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, Wisconsin, 53706, U.S.A

    Robert E. Hanneman Jr.

Authors
  1. Kazuyoshi Hosaka
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  2. Robert E. Hanneman Jr.
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Hosaka, K., Hanneman, R. Genetics of self-compatibility in a self-incompatible wild diploid potato species Solanum chacoense. 2. Localization of an S locus inhibitor (Sli) gene on the potato genome using DNA markers. Euphytica 103, 265–271 (1998). https://doi.org/10.1023/A:1018380725160

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  • DOI: https://doi.org/10.1023/A:1018380725160

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