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Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects

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Abstract

Immobilized a-chymotrypsin was used as catalyst for studying temperature effects on acyl transfer reactions (acyl-donor: Bz-TyrOEt) in a water-immiscible organic solvent. The solubility of the two nucleophiles, Phe-NH and water, decreased with decreasing temperature. The relative decrease for the amide was larger (2.4-fold) than for water. Therefore the thermodynamic activity (estimated by the relative saturation) increased more for this substrate and hence the selectivity in the reaction was increased.

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References

  • Chen, S-T., Chen, S-Y., Kao, C-L. and Wang, K-T. (1994). Biotechnol. Lett. 16, 1075–80.

    Google Scholar 

  • Janssen, A.E.M. and Halling, P.J. (1994). J. Am. Chem. Soc. 116, 9827–9830.

    Google Scholar 

  • Jönsson, Å., Adlercreutz, P. and Mattiasson, B. (1995). Biotechnol. Bioeng. 46, 429–436.

    Google Scholar 

  • Jönsson, Å., Wehtje, E. Adlercreutz, P. and Mattiasson, B. (1996). J. Mol. Catalysis. B: Enzymatic. 2, 43–51.

    Google Scholar 

  • Wescott, C.R. and Klibanov, A.M. (1993). J. Am. Chem. Soc. 115, 1629–31.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-22100, Lund, Sweden

    Åsa Jo¨nsson, Ernst Wehtje & Patrick Adlercreutz

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  1. Åsa Jo¨nsson
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  2. Ernst Wehtje
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  3. Patrick Adlercreutz
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Jo¨nsson, Å., Wehtje, E. & Adlercreutz, P. Low reaction temperature increases the selectivity in an enzymatic reaction due to substrate solvation effects. Biotechnology Letters 19, 85–88 (1997). https://doi.org/10.1023/A:1018379423480

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  • DOI: https://doi.org/10.1023/A:1018379423480

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