Abstract
Polyploidy in blackberries and ploidy differences between Rubus species are obstacles to the efficient introduction of valuable germplasm, both intra- and interspecific, into blackberry breeding programs. Expansion of the germplasm base would be facilitated by reducing the ploidy level of blackberry cultivars to the diploid level. In this report selection of twin seed, interspecific hybridization, and pollen irradiation were compared as methods in the recovery of dihaploids from tetraploid blackberry cultivars. One dihaploid was obtained through selection of twin seed and several were obtained following interspecific hybridization. The infrequency of twinning and difficulty in detecting twin seed in Rubus reduced the efficiency of this method. The efficiency with which dihaploids could be obtained following interspecific hybridization varied with the pollen parent. Reduced seed set and seed quality following pollinations with respectively R. parvifolius and R. hirsutus could be used to advantage in the recovery of dihaploids from blackberries. Ploidy reduction in several tetraploid blackberry cultivars was obtained following pollinations with 100 and 150 kR gamma irradiated pollen. Most of the seedlings obtained at the 50 kR dosage were aneuploid. Pollen irradiation at 150 kR was the most efficient method of obtaining dihaploids from tetraploid blackberries. Twenty percent of the seedlings obtained following this treatment were dihaploid.
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Beversdorf, W.D. & E.T. Bingham, 1977. Male-sterility as a source of haploids and polyploids of Glycine max. Can J Genet Cytol 19: 283–287.
Bingham, E.T., 1971. Isolation of haploids of tetraploid alfalfa. Crop Sci 11: 433–435.
Britton, D.M. & J.W. Hull, 1957. Mitotic instability in Rubus. J Hered 48: 11–20.
Bouvier, L., Y.Z. Zhang & Y. Lespinasse, 1993. Two methods of haploidization in pear, Pyrus communis L.: Greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen. Theor Appl Genet 87: 229–232.
Chase, S.S., 1969. Monoploids and monoploid-derivatives of maize (Zea mays L.). Bot Rev 35: 117–167.
Chu, C.C., 1982. Haploids in plant improvement. In: I.K. Vasil, W.R. Snowcroft & K.J. Frey (Eds.), Plant Improvement and Somatic Cell Genetics, pp. 129–158. Academic Press, New York, NY.
Crane, M.B. & P.T. Thomas, 1949. Reproductive versatility in Rubus. III. Raspberry-blackberry hybrids. Heredity 3: 99–107.
Cuny, F., M. Grotte, R. Dumas De Vaulx & A. Rieu, 1993. Effects of gamma irradiation of pollen on parthenogenetic haploid production in muskmelon (Cucumis melo L.). Environ Exp Bot 33: 301–312.
Doyle, J.J. & J.L. Doyle, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bul 19: 11–15.
Dweikat, I.M. & P.M. Lyrene, 1990. Twin seedlings and haploids in blueberry (Vaccinium spp.). J Hered 1990: 198–200.
Einset, J. & C. Pratt, 1954. Hybrids between blackberries and red raspberries. Proc Am Soc Hort Sci 63: 257–261.
Hougas, R.W., S.J. Peloquin & R.W. Ross, 1958. Haploids of the common potato. J Hered 49: 103–106.
James, D.J., A.J. Passey, K.A.D. Mackenzie & E.C. Menhinick, 1985. The effects of pollen irradiation on the development of the post-fertilization ovule of apple (Malus pumila Mill.). In: G.P. Chapman, S.H. Mantell & P.W. Daniels (Eds.), Experimental Manipulation of Ovule Tissue, pp. 210–223. Longman, London.
Jennings, D.L., 1988. Raspberries and Blackberries: Their Breeding Diseases and Growth, p. 50. Academic Press, London and New York.
Jennings, D.L., D.L. Craig & P.B. Topham, 1967. The role of the male parent in the reproduction of Rubus. Heredity 22: 43–55.
Johnston, S.A., T.P.M. den Nijs, S.J. Peloquin & R.E. Hanneman, Jr., 1980. The significance of genic balance to endosperm development in interspecific crosses. Theor Appl Genet 57: 5–9.
Kasha, K.J., 1974. Haploids from somatic cells. In: K.J. Kasha (Ed.), Haploids from Higher Plants, pp. 67–87. University of Guelph, Guelph, Ontario, Canada.
Keijzer, C.J. & M.T.M. Willemse, 1989. Cell isolation, recognition and fusion during sexual reproduction. In: D.J. Osborne & M.B. Jackson (Eds.), Cell Separation in Plants, pp. 275–285. Springer Verlag, Berlin.
Kimber, G. & R. Riley, 1963. Haploid angiosperms. Bot Rev 29: 480–531.
Lacadena, J.R., 1974. Spontaneous and induced parthenogenesis and androgenesis. In: K. J. Kasha (Ed.), Haploids in Higher Plants, pp. 13–32. University of Guelph, Guelph, Ontario, Canada.
Lespinasse, Y., M. Godicheau & M. Duron, 1983. Potential value and method of producing haploids in the apple tree, Malus pumila (Mill.). Acta Horticulturae 131: 223–230.
Levi, A., L. J. Rowland & J. S. Hartung, 1993. Production of reliable randomly amplified polymorphic DNA (RAPD) markers from DNA of woody plants. HortScience 28: 1188–1190.
Lloyd, G. & B. H. McCown, 1980. Commercially-feasible micro-propagation of Mountain Laurel, Kalmia latifolia, by use of shoot-tip culture. Proc Int Plant Prop Soc 30: 421–427.
Magoon, M.L. & K.R. Khanna, 1963. Haploids. Caryologia 16: 191–235.
McGrath, J.M., S.M. Wielgus, T.F. Uchytil, H. Kim-Lee, G.T. Haberlack, C.E. Williams & J.P. Helgeson, 1994. Recombination of Solanum brevidens chromosomes in the second backcross gener-ation from a somatic hybrid with S. tuberosum. Theor Appl Genet 88: 917–924.
McGrath, J.M., S.M. Wielgus & J.P. Helgeson, 1996. Segregation and recombination of Solanum brevidens synteny groups in progeny of somatic hybrids with S. tuberosum: Intragenomic equals or exceeds intergenomic recombination. Genetics 142: 1335–1348.
Moore, J.N., 1980. Blackberry production and cultivar situation in North America. Fruit Var J 34: 36–41.
Moore, J.N., G.R. Brown & C. Lundergan, 1974. Effect of duration of acid scarification on endocarp thickness and seedling emergence of blackberries. HortScience 9: 204–205.
Morgan, D.T. & R.D. Rappleye, 1951. Polyembryony in maize and lily. J Hered 42: 90–93.
Muren, R.C., T.M. Ching & K.K. Ching, 1979. Metabolic study of Douglas-Fir pollen germination in vitro. Phys Plantarum 46: 287–292.
Naess, S.K., 1995. Ploidy Reduction in Blackberry. Ph D Thesis, University of Maryland, College Park, Maryland, 165 pp.
Nicoll, M.F., G.P. Chapman & D.J. James, 1987. Endosperm responses to irradiated pollen in apples. Theor Appl Genet 74: 508–515.
Pandey, K.K., 1975. Sexual transfer of specific genes without gametic fusion. Nature 256: 310–313.
Pandey, K.K., 1980. Further evidence for egg transformation in Nicotiana. Heredity 45: 15–29.
Rowland, L.J. & B. Nguyen, 1993. Use of PEG for purification of DNA from leaf tissue of woody plants. Bio Techniques 14: 735–736.
Stettler, R.F. & K.S. Bawa, 1971. Experimental induction of haploid parthenogenesis in black cottonwood. Silv Genet 20: 15–25.
Thompson, M.M., 1962. Cytogenetics in Rubus III. Meiotic instability in some higher polyploids. Am J Bot 49: 575–582.
Thompson, T.E., 1977. Haploid breeding technique in flax. Crop Sci 17: 757–760.
Turcotte, E.L. & C.V. Feaster, 1969. Semigametic production of haploids in pima cotton. Crop Sci 9: 653–655.
Vassileva-Dryanovska, O.A., 1966. Development of embryo and endosperm produced after irradiation of pollen in Tradescantia. Hereditas 55: 129–147.
Welsh, J. & M. McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 18: 7213–7218.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Raflske & S.V. Tingey, 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535.
Wilson, J.A. & W.M. Ross, 1961. Haploidy in twin seedlings of winter wheat, Triticum aestivum. Crop Sci 1: 82.
Yang, H.Y. & C. Zhou, 1982. In vitro induction of haploid plants from unpollinated ovaries and ovules. Theor Appl Genet 63: 97–104.
Yarnell, S.H. & H.T. Blackhurst, 1947. A polyploid chromosome series from a cross of the Lawton blackberry and the Nessberry. Proc Am Soc Hort Sci 49: 189–192.
Zhang, Y.X. & Y. Lespinasse, 1991. Pollination with gamma-irradiated pollen and development of fruits, seeds and parthenogenetic plants in apple. Euphytica 54: 101–109.
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Naess, S.K., Swartz, H.J. & Bauchan, G.R. Ploidy reduction in blackberry. Euphytica 99, 57–73 (1998). https://doi.org/10.1023/A:1018372709235
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DOI: https://doi.org/10.1023/A:1018372709235