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The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

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Abstract

The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.

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Authors and Affiliations

  1. Molecular Genetics Program, Department of Botany and Microbiology, Auburn University, 101 Life Sciences Building, Auburn, AL, 36849–5407, USA

    P.J. Brixey, C. Guda & H. Daniell

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  1. P.J. Brixey
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  2. C. Guda
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  3. H. Daniell
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Brixey, P., Guda, C. & Daniell, H. The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein. Biotechnology Letters 19, 395–400 (1997). https://doi.org/10.1023/A:1018371405675

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  • DOI: https://doi.org/10.1023/A:1018371405675

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