Abstract
The use of a diffusion filter is proposed to suppress the NMRsignals of small organic compounds in the presence of macromolecules.Combined with a spin-echo relaxation filter, the diffusion filter enablesthe selective and simultaneous detection of intermolecularsolvent–protein NOEs in a straightforward two-dimensional NOESYexperiment. Using the intermolecular NOEs observed betweenN,N-dimethylformamide (DMF) and hen egg-white lysozyme in an aqueoussolution containing 2 M DMF, the binding of DMF at thespecificity-determining substrate binding site C of the enzyme was modelled.
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Ponstingl, H., Otting, G. Detection of protein–ligand NOEs with small, weakly binding ligands by combined relaxation and diffusion filtering. J Biomol NMR 9, 441–444 (1997). https://doi.org/10.1023/A:1018363030177
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DOI: https://doi.org/10.1023/A:1018363030177