Skip to main content

Advertisement

SpringerLink
Log in

Biochemical synthesis of chirally pure Rp oligonucleotide phosphorothioates

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Oligonucleotide phosphorothioates have been synthesized using procaryotic DNA polymerase and oligonucleotide template/primer. The method facilitates the recovery of DNA polymerase and template/primer and is successful at the milligram scale.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Eckstein F. (1983). Angewandte Chemie, 22, 423–439.

    Article  Google Scholar 

  • Maran A., Maitra R.K., Kumar A., Dong B., Xiao W., Li G., Williams B.R.G., Torrence P.F. and Silverman R.H. (1994). Science, 265, 789–792.

    Article  PubMed  CAS  Google Scholar 

  • Matsukura M., Shinozuka K., Zon G., Mitsuya H., Reitz M., Cohen J.S. and Broder S. (1987). Proc. Natl. Acad. Sci. USA, 84, 7706–7710.

    Article  PubMed  CAS  Google Scholar 

  • Nelson N., Reynolds M. and Arnold L. (1992). Detection of Acridinium Esters by Chemiluminescence. In: Nonisotopic DNA Probe Techniques, L.J. Kricka ed., pp. 275–311, San Diego, California: Academic Press.

    Google Scholar 

  • Stein C.A. and Cheng Y.-C. (1993). Science, 261, 1004–1012.

    Article  PubMed  CAS  Google Scholar 

  • Tang J., Roskey A., Li Y. and Agrawal S. (1995). Nucleosides & Nucleotides, 82, 597–602.

    Google Scholar 

  • Tuerk C., MacDougal S. and Gold L. (1992). Proc. Natl. Acad. Sci. USA, 89, 6988–6992.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chugai Biopharmaceuticals, 6275 Nancy Ridge Drive, San Diego, CA, 92121, USA

    David Bruce Lackey & Jasmin Patel

Authors
  1. David Bruce Lackey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jasmin Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lackey, D.B., Patel, J. Biochemical synthesis of chirally pure Rp oligonucleotide phosphorothioates. Biotechnology Letters 19, 475–478 (1997). https://doi.org/10.1023/A:1018356429782

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018356429782

Keywords

Search

Navigation