Colisa lalia (Ham.) has been used as a model for the development of techniques for induced spawning that are applicable to small teleosts where ovulation requires prolonged exposure to suitable breeding conditions.
Human chorionic gonadotrophin (hCG; ≥ 4 IU per fish) induced ovulation within 24 hours, whereas homologous pituitary extracts were relatively ineffective. When administered in saline (≤ 20 μg per fish per injection), des-Gly10,[D-Ala6]- LHRH N-ethylamide (LHRHa) was ineffective, but it stimulated ovulation in a proportion of fish when administered (≥ 1.6 μg per fish) as an emulsion in Freund's incomplete adjuvant (FIA). Together, these results suggest that ovulation requires the synthesis as well as the secretion of gonadotrophin in C. lalia.
Long-term treatment with thyroid hormones appeared to enhance the ovulatory response to LHRHa in FIA, possibly by effects on the ovary; whereas the various steroids tested were ineffective at the dosages used
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Aida, K. (1988) A review of plasma hormone changes during ovulation in cyprinid fishes. Aquaculture 74, 11–21.
Aida, K., Izumo, R.S., Satoh, H. and Hibiya, T. (1978) Induction of ovulation in plaice and goby by synthetic LH-releasing hormone. Bulletin of the Japanese Society for Scientific Fisheries 44, 445–450 (in Japanese, with English abstract).
Amano, M., Hyodo, A., Urano, S., Okumoto, N., Kitamura, S., Ikuta, K., Suzuki, Y. and Aida, K. (1994) Activation of salmon gonadotropin-releasing hormone synthesis by 17α-methyltestosterone administration in yearling masu salmon, Oncorhynchus masou. General and Comparative Endocrinology 95, 374–380.
Baerends, G.P. (1986) On causation and function of the pre-spawning behaviour of cichlid fish. Journal of Fish Biology 29 (Supplement A), 107–12
Billard, R. and Breton, B. (1978) Rhythms of reproduction in teleost fish. In Thorpe, J.E., ed., Rhythmic Activity of Fishes, London: Academic Press, pp. 31–5
Billard, R., Reinaud, P., Hollebecq, M.G. and Breton, B. (1984) S. trutta following administration of LRH-A combined or not with pimozide}. Aquaculture 43, 57–66.
Crim, L.W., Sherwood, N.M. and Wilson, C.E. (1988) Sustained hormone release. II. Effectiveness of LHRH analogue (LHRHa) administration by either single time injection or cholesterol pellet implantation on plasma gonadotropin levels in a bioassay model fish, the juvenile rainbow trout. Aquaculture 74, 87–95.
Detlaf, T.A. and Davydova, S.I. (1975) Effect of tri-iodothyronine on ripening of oocytes in stellate sturgeon after the action of low temperatures and reservation of females. Ontogenez 5, 454–462.
Dickey, J.T. and Swanson, P. (1995) Development of RNase protection assays for quantification of gonadotrophin (GTH I and GTH II) subunit transcript levels in coho salmon (Oncorhynchus kisutch). In Goetz, F.W. and Thomas, P., eds, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, p. 28.
Donaldson, E.M. and Hunter, G.A. (1983) Induced final maturation, ovulation and spermiation in cultured fish. In Hoar, W.S., Randall, D.J. and Donaldson, E.M., eds, Fish Physiology, Volume 9B, New York: Academic Press, pp. 331–374.
Drori, S., Ofir, M., Levavi-Sivan, B. and Yaron, Z. (1994) Spawning induction in common carp (Cyprinus carpio) using pituitary extract or GnRH superactive analogue combined with metoclopramide: analysis of hormone profile, progress of oocyte maturation and dependence on temperature. Aquaculture 119, 393–40
Dufour, S., Bassompierre, M., Montero, M., le Belle, N., Baloche, S. and Fontaine, Y.A. (1991) Stimulation of pituitary gonadotrophic function in female silver eel treated by a gonadoliberin agonist and dopamine antagonists. In Scott, A.P., Sumpter, J.P., Kime, D.E. and Rolfe, M.S., eds, Reproductive Physiology of Fishes, Sheffield, UK: FishSymp91}, pp. 54–56
Eales, J.G. (1974) Creation of chronic elevations of plasma thyroxine in brook trout Salvelinus fontinalis (Mitchill) and other teleosts. General and Comparative Endocrinology 22, 209–217.
Gur, G., Melamed, P., Levavi-Sivan, B., Holland, C., Gissis, A. Bayer, D., Elizur, A., Zohar, Y. and Yaron, Z. (1995) Longterm testoterone treatment stimulates GTH II synthesis and release in the pituitary of the black carp, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, p. 32.
Habibi, H.R. (1991a) Desensitization to native molecular forms of gonadotropin-releasing hormone in the goldfish pituitary: dependence on pulse frequency and concentration. General and Comparative Endocrinology 84, 199–21
Habibi, H.R. (1991b) Homologous desensitization of gonadotropin-releasing hormone (GnRH) receptors in the goldfish pituitary: effects of native GnRH peptides and a synthetic GnRH antagonist. Biology of Reproduction 44, 275–28
Huggard, D. and Habibi, H.R. (1995) Effect of testosterone on growth hormone and maturational gonadotrophin subunit gene expression in the cultured goldfish pituitary in vitro. In Goetz, F.W. and Thomas, P., eds, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, pp. 19–21
Jalabert, B. (1976) In vitro oocyte maturation and ovulation in rainbow trout (Salmo gairdneri), northern pike (Esox lucius) and goldfish (Carassius auratus). Journal of the Fisheries Research Board of Canada 33, 974–98
Kagawa, H., Tanaka, H., Okuzawa, K. and Hirose, K. (1994) Development of maturational competence of oocytes of red seabream, Pagrus major, after human chorionic gonadotropin treatment in vitro requires RNA and protein synthesis. General and Comparative Endocrinology 94, 199–206.
Kah, O., Anglade, I., Leprêtre, E., Dubourg, P. and de Monbrison, D. (1993) The reproductive brain in fish. Fish Physiology and Biochemistry 11, 85–9
King, W., Thomas, P., Harrell, R.M., Hodson, R.G. and Sullivan, C.V. (1994) Plasma levels of gonadal steroids during final oocyte maturation of striped bass, Morone saxatilis L. General and Comparative Endocrinology 95, 178–19
Kobayashi, M., Aida, K. and Hanyu, I. (1987) Hormone changes during ovulation and effects of steroid hormones on plasma gonadotropin levels and ovulation in goldfish. General and Comparative Endocrinology 67, 24–3
van der Kraak, G., Donaldson, E.M. and Chang, J.P. (1986) Dopamine involvement in the regulation of gonadotropin secretion in coho salmon. Canadian Journal Of Zoology 64, 1245–1248.
Lam, T.J. (1982) Applications of endocrinology to fish culture. Canadian Journal of Fisheries and Aquatic Sciences 39, 111–13
Lee, C.S., Tamaru, C.S. and Kelley, C.D. (1986) Technique for making chronic-release LHRHa and 17α-methyltestosterone pellets for intramuscular implantation in fishes. Aquaculture 59, 161–16
Lee, C.S., Tamaru, C.S. and Kelley, C.D. (1988) The cost and effectiveness of CPH, HCG and LHRH-a on the induced spawning of grey mullet, Mugil cephalus. Aquaculture 73, 341–347.
de Leeuw, R., Resink, J.W., Rooyakkers, E.J.M. and Goos, H.J.T. (1985) Pimozide modulates the luteinizing hormone-releasing hormone effect on gonadotropin release in the African catfish Clarias lazera. General and Comparative Endocrinology 58, 120–127.
Marte, C.L., Sherwood, N.M., Crim, L.W. and Tan, J. (1988) Induced spawning of maturing milkfish (Chanos chanos) using human chorionic gonadotropin and mammalian and salmon gonadotropin-releasing hormone analogues. Aquaculture 73, 333–34
Melamed, P., Gur, G., Levavi-Sivan, B., Elizur, A. and Yaron, Z. (1995) Intracellular mediation of GnRH effect on transcription of the tilapia GTH IIβ gene. In Goetz, F.W. and Thomas, P., eds, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, p. 32.
Munro, A.D. (1990a) General Introduction. In Munro, A.D., Scott, A.P. and Lam, T.J., eds, The Environment and the Control of Reproduction in Teleost Fish, Boca Raton, FL: CRC Press, pp. 1–12.
Munro, A.D. (1990b) Tropical freshwater teleosts. In Munro, A.D., Scott, A.P. and Lam, T.J., eds, The Environment and the Control of Reproduction in Teleost Fish, Boca Raton, FL: CRC Press, pp. 145–239.
Munro, A.D. and Lam, T.J. (1995) Control of gonad growth, maturation and spawning in teleost fish: a review. In Marte, C.L., Quinitio, G. and Amata, A.C., eds, Proceedings of the Seminar-Workshop on Finfish Breeding and Seed Production in the Philippines}, Iloilo, The Philippines: SEAFDEC}, 1–80
Mylonas, C.C., Sullivan, C.V. and Hinshaw, J.M. (1994) Thyroid hormones in brown trout (Salmo trutta) reproduction and early development. Fish Physiology and Biochemistry 13, 485–493.
Ngamvongchon, S., Pawaputanon, O., Leelapatra, W. and Johnson, W.E. (1988) Effectiveness of an LHRH analogue for the induced spawning of carp and catfish in northeast Thailand. Aquaculture 74, 35–40.
Pankhurst, N.W. (1985) Final maturation and ovulation of oocytes of the goldeye, Hiodon alosoides (Rafinesque), in vitro. Canadian Journal of Zoology 63, 1003–1009
Peter, R.E. and Yu, K.L. (1997) Neuroendocrine regulation of ovulation in fishes: basic and applied aspects. Reviews in Fish Biology and Fisheries, 7, 000–00
Peter, R.E., Chang, J.P., Nahorniak, C.S., Omeljaniuk, R.J., Sokolowska, M., Shih, S.H. and Billard, R. (1986) Interaction of catecholamines and GnRH in the regulation of gonadotropin secretion in teleost fish. Recent Progress in Hormone Research 42, 513–564.
Peter, R.E., Lin, H.-R. and van der Kraak, G. (1988) Induced ovulation and spawning of cultured freshwater fish in China: advances in application of GnRH analogues and dopamine antagonists. Aquaculture 74, 1–10.
Peter, R.E., Trudeau, V.L., Sloley, B.D., Peng, C. and Nahorniak, C.S. (1991) Actions of catecholamines, peptides and sex steroids in regulation of gonadotropin-II in the goldfish. In Scott, A.P., Sumpter, J.P., Kime, D.E. and Rolfe, M.S., eds, Reproductive Physiology of Fishes}, Sheffield, UK: FishSymp} 91}, pp. 30–34
Richter, C.J.J., Eding, E.H., Goos, H.J.T., de Leeuw, R., Scott, A.P. and van Oordt, P.G.W.J. (1987) The effect of pimozide/LHRHa and 17α-hydroxyprogesterone on plasma steroid levels and ovulation in the African catfish, Clarias gariepinus. Aquaculture 63, 157–168
Ros, A.E., Amutio, V.G., Arceredillo, J.P.M., Orti, G. and Nani, A. (1984) Induced breeding of the South American catfish Rhamdia sapo (C. & V.). Aquaculture 37, 141–146.
Rowland, S.J. (1983) The hormone-induced ovulation and spawning of the Australian freshwater fish golden eye perch, Macquaria ambigua (Richardson) (Percichthyidae). Aquaculture 35, 221–238.
Schultz, R., Bogerd, J., Bosma, P.T., Peute, J., Rebers, F.E.M., Zandbergen, M.A. and Goos, H.J.T. (1995) Physiological, morphological and molecular aspects of gonadotrophins in fish, with special reference to the African catfish Clarias gariepinus. In Goetz, F.W. and Thomas, P., eds, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, pp. 2–6.
Sherwood, N.M., Crim, L.W., Carolsfeld, J. and Walters, S.M. (1988) Sustained hormone release. I. Characteristics of in vitro release of gonadotropin-releasing hormone analogue (GnRH-A) from pellets. Aquaculture 74, 75–86.
Sullivan, C.V., Bernard, M.G., Hara, A. and Dickhoff, W.W. (1989) Thyroid hormones in trout reproduction: enhancement of gonadotropin-releasing hormone analogue and partially purified salmon gonadotropin-induced ovarian maturation in vivo and in vitro. Journal of Experimental Zoology 250, 188–195.
SwansonP.1991Salmongonadotropinsreconcilingoldandnewideas.InScottA.P.SumpterJ.P.KimeD.E.andRolfeM.S.edsReproductivePhysiologyofFishesSheffieldUKFishSymp91, pp. 2–
Teo, P.L.J. (1984) Influence of male Colisa lalia (dwarf gourami) on the ovarian cycle of the female. Unpubl. Honours thesis, National University of Singapore, 75 pp.
Thalathiah bte. Saidin, Ashhar, A. and Zaini, M. (1988) Induced spawning techniques practised at Batu Berendam, Melaka, Malaysia. Aquaculture 74, 23–33.
Trudeau, V.L. and Peter, R.E. (1995) Functional interactions between neuroendocrine systems regulating GTH-II release. In Goetz, F.W. and Thomas, P., eds, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, pp. 44–48.
Tsi, D., Ng, N.K. and Munro, A.D. (1994) The role of an LHRH analogue and monoamines in the induction of ovulation in the dwarf gourami (Colisa lalia). In Chou, L.M., Munro, A.D., Lam, T.J., Chen, T.W., Cheong, L.K.K., Ding, J.L., Hooi, K.K., Khoo, H.W., Phang, V.P.E., Shim, K.F. and Tan, C.H., eds, Proceedings of Third Asian Fisheries Forum, Manila, Philippines: Asian Fisheries Society, pp. 872–875.
Tyler, C.R., Sumpter, J.P. and Witthames, P.R. (1990) The dynamics of oocyte growth during vitellogenesis in the rainbow trout (Oncorhynchus mykiss). Biology of Reproduction 43, 202–209.
Venkatesh, B., Tan, C.H. and Lam, T.J. (1991) Progestins and cortisol delay while oestradiol-17β induces early parturition in the guppy, Poecilia reticulata. General and Comparative Endocrinology 83, 297–305.
Yamamoto, N., Oka, Y., Amano, M., Aida, K., Hasegawa, Y. and Kawashima, S. (1995) Multiple gonadotrophin-releasing hormone (GnRH)-immunoreactive systems in the brain of the dwarf gourami, Colisa lalia: immunohistochemistry and radio-immunoassay. Journal of Comparative Neurology 355, 354–368.
Yaron, Z., Gur, G., Melamed, P., Levavi-Sivan, B., Gissis, A., Bayer, D., Elizur, A., Holland, C., Zohar, Y. and Schriebman, M.P. (1995) Blocks along the hypothalamo-hypophyseal-gonad axis in immature black carp, Mylopharyngodon piceus. In Goetz, F.W. and Thomas, P., eds, Proceedings of the Fifth International Symposium on Reproductive Physiology of Fish, Austin, Texas: FishSymp95, p. 22.
Zairin, M., Asahina, K., Furukawa, K. and Aida, K. (1992) Plasma steroid hormone profiles during HCG-induced ovulation in female walking catfish, Clarias batrachus. Zoological Science 9, 607–617.
Zheng, W.B., Pan, J.H. and Liu, W.S. (1988) Culture of catfish in China. Aquaculture 75, 35–44.
Zohar, Y. (1989) Fish physiology: its physiology and artificial regulation. In Shilo, M. and Sarig, S., eds, Fish Culture in Warm Water Systems: Problems and Trends, Boca Raton, FL: CRC Press, pp. 102–163.
Zohar, Y., Goren, A., Tosky, M., Pagelson, G., Leibovitz, D. and Koch, Y. (1989) The bioactivity of gonadotropin releasing hormones and its regulation in the gilthead seabream, Sparus aurata: in vivo and in vitro studies. Fish Physiology and Biochemistry 7}, 59–
Zohar, Y., Pagelson, G., Gothilf, Y., Dickhoff, W.W., Swanson, P., Duguay, S., Gombotz, W., Kost, J. and Langer, R. (1990) Controlled release of gonadotropin releasing hormones for the manipulation of spawning in farmed fish. Proceedings, International Symposia on Controllable Release and Bioactive Materials 17, 833–834.
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Ng , N., Tsi , D. & Munro , A. Induced final maturation and ovulation in a small anabantoid teleost, the dwarf gourami, Colisa lalia. I. The effects of gonadotrophic preparations, LHRHa, steroids and thyroid hormones. Aquarium Sciences and Conservation 1, 169–188 (1997). https://doi.org/10.1023/A:1018352121469
- Colisa lalia
- gonadotrophin-releasing factor
- induced ovulation
- thyroid hormones