Abstract
Three isozyme sytems glutamate oxaloacetate transaminase (GOT), superoxide dismutase (SOD), and malic enzyme (ME) have been analysed electrophoretically to study the diversity within and between 33 faba bean (V. faba L. type minor) accessions. For comparison, two cultivars (Vesuvio and Talo) were included in the study. Further objective of this study was to classify populations into genetic similar groups. SOD and GOT showed large variation, while ME presented minor change. Principal components, hierarchical cluster, and canonical discriminant analyses produced five groups with significant differences among them. The alleles with low frequency played a significant role in the discrimination. It was concluded that more detailed investigations regarding genetic aspects of enzyme diversity are needed to support the data here reported.
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References
Amet, T.M., 1986. Geographical patterns of allozymes variation in a germplasm collection of faba bean (Vicia faba L.). FABIS Newslett 16: 5–12.
Andersen, W.R. & D.J. Fairbanks, 1990. Molecular markers: Important tools for plant genetic resource characterization. Diversity 6(3–4): 51–53.
Bianco, V.V., G. Damato, V. Miccolis, G.B. Polignano, E. Proceddu & G. Scippa, 1979. Variation in a collection of Vicia faba L. and correlations among agronomically important characters. In: D.A. Bond, G.T. Scarascia Mugnozza & M.K. Poulsen (Eds), Some current research on Vicia faba L. in Western Europe, pp. 217–250. EEC, EUR 6244 EN Luxembourg.
Birouk, A. & Y. Dattée. 1989. Organisation de la variabilité enzymatique des populations marocaines de luzerne (Medicago sativa L.): structures géniques et génotypiques. Genome 32: 120–128.
Carré, S., J.N. Tasei, J. Le Guen, J. Mesquida & G. Morin, 1993. The genetic control of seven isozymic loci in Vicia faba L. Identification of lines and estimates of outcrossing rates between plants pollinated by bumble bees. Ann Appl Biol 122: 555–568.
Fanizza, G., G. Sonnante & A. Spinosa, 1995. Studio sulla diversità di ecotipi di Prunus webbii diffusi in Puglia. Italus Hortus 2(1, 2): 53–56.
Greco, I., 1993. Isoenzymic characterization of strawberry clones in South Italy. Acta Hort 345: 21–27.
Kephart, S.R., 1990. Starch gel electrophoresis of plant isozymes: a comparative analysis of techniques. Amer J Bot 77(5): 693–712.
Koenig, R. & P. Gepts, 1989. Allozyme diversity in wild Phaseolus vulgaris: further evidence for two major centers of genetic diversity. Theor Appl Genet 78: 809–817.
Mancini, R., C. De Pace, G.T. Scarascia Mugnozza, V. Del Re & D. Vittori, 1989. Isozyme gene markers in Vicia faba L. Theor Appl Genet 77: 657–667.
Panella, L. & P. Gepts, 1992. Genetic relationships within Vigna unguiculata (L.) Walp. based on isozyme analyses. Gen Res Crop Evol 39: 71–88.
Polignano, G.B., D. Lafiandra, V. Miccolis & G. Taranto, 1979. Variabilità di caratteri agronomici e del contenuto proteico in una collezione di Vicia faba L. In: Incontro-Seminario sulla attività di ricerca nei settori del frumento duro e delle leguminose da granella. 27–28 october 1979, Enna, Italy. Galatea Editrice, Acireale, Catania: 295–311.
Polignano, G.B., R. Splendido & P. Perrino, 1990. Seed storage proteins diversity in faba bean (Vicia faba L.) entries from Ethiopia and Afghanistan. J Genet Breed 44: 31–38.
Polignano, G.B. & G. Sonnante, 1992. Characterization of faba bean populations by isozyme patterns of GOT and PGI. FABIS Newslett 31: 12–16.
Polignano, G.B. & P. Uggenti, 1989. Variation analysis of physiological traits among different entries of faba bean (Vicia faba L.). FABIS Newslett 25: 22–26.
Raelson, J.V. & W.F. Grant, 1989. An isoenzyme study in the genus Lotus (Fabaceae). Experimental protocols and genetic basis of electrophoretic phenotype. Theor Appl Genet 77: 595–607.
Sadocchi, S., 1990. Manuale di analisi statistica multivariata. Franco Angeli Libri s.r.l., Milano Italy, pp. 204–206.
SAS Institute, Inc., 1985. Statistics. Cary, NC.,USA.
Souza, E. & M.E. Sorrells, 1991a. Relationships among 70 North American oat germplasm: I. Cluster analysis using quantitative characters. Crop Sci 31: 599–605.
Souza, E. & M.E. Sorrells, 1991b. Relationships among 70 North American oat Germplasm: II. Cluster analysis using qualitative characters. Crop Sci 31: 605–612.
Suso, M.J. & M.T. Moreno, 1986. Isoenzymatic polymorphism of superoxide dismutase (SOD) in Vicia faba and its systematic implication. FABIS Newslett 16: 3–11.
Suso, M.J., M.T. Moreno & J.I. Cubero, 1993. New isozyme markers in Vicia faba: inheritance and linkage. Plant Breeding 111: 170–172.
Torres, A.M., Z. Satovic, J. Canovas, S. Cobos & J.I. Cubero, 1995. Genetics and mapping of new isozyme loci in Vicia faba L. using trisomics. Theor Appl Genet 91: 783–789.
Torres, A.M., N.F. Weeden & A. Martin, 1993. Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85: 937–945.
Weeden, N.F., 1984. Linkage between the gene coding the small subunit of ribulose biphosphate carboxilase and the gene coding malic enzyme in Phaseolus vulgaris. Ann Rept Bean Improv Coop 27: 123–125.
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Polignano, G., Quintano, G., Bisignano, V. et al. Enzyme polymorphism in faba bean (Vicia faba L. minor) accessions. Genetic interpretation and value for classification. Euphytica 102, 169–176 (1998). https://doi.org/10.1023/A:1018340228500
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DOI: https://doi.org/10.1023/A:1018340228500