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Inhibition of 5-aminolevulinic acid (ALA) dehydratase by undissociated levulinic acid during ALA extracellular formation by Rhodobacter sphaeroides

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Abstract

The inhibition of ALA dehydratase by levulinic acid during ALA extracellular formation of Rhodobacter sphaeroides correlated with the concentration of undissociated form of levulinic acid irrespective of culture pH. The inhibition constant, Ki, of intracellular ALA dehydratase by Dixon plots was 2.95 μM. Undissociated levulinic acid therefore functions as an inhibitor of ALA dehydratase.

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Authors and Affiliations

  1. Chemistry Laboratory, Hiroshima-Denki Institute of Technology, Nakamo, Akiku Hiroshima, 739-03, Japan

    Ken Sasaki

  2. Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Kagamiyama Higashi-Hiroshima, 739, Japan

    Masanori Watanabe & Naomichi Nishio

Authors
  1. Ken Sasaki
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  2. Masanori Watanabe
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  3. Naomichi Nishio
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Sasaki, K., Watanabe, M. & Nishio, N. Inhibition of 5-aminolevulinic acid (ALA) dehydratase by undissociated levulinic acid during ALA extracellular formation by Rhodobacter sphaeroides. Biotechnology Letters 19, 421–424 (1997). https://doi.org/10.1023/A:1018331824331

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  • DOI: https://doi.org/10.1023/A:1018331824331

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