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Enzymes catalyzed esterification of N-protected amino acids with secondary alcohols

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Abstract

Secondary alcohols were converted to their corresponding esters with N-Cbz-L-amino acids by Celite-immobilized protease or lipase in organic solvents at pH 7.5. The esterification of 2-butanol and 2-phenylethanol were achieved up to 71% yields. The optimal reaction condition for Aspergillus oryzae protease (AOP) catalyzed synthesis of N-Cbz-L-aspartyl- sec-butyl ester (97.6 de) was at pH 7.5 and a duration of 96 hours.

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Authors and Affiliations

  1. Biochemical Engineering Center, Da-Yeh Institute of Technology, Chang Hwa, Taiwan

    I.-L. Shih, L.-C. Chiu & C.T. Lai

  2. Department of Chemical Engineering, National Yunlin Institute of Technology, Yunlin, Taiwan

    W.-C. Liaw

  3. Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan ROC

    D.-F. Tai

Authors
  1. I.-L. Shih
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  2. L.-C. Chiu
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  3. C.T. Lai
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  4. W.-C. Liaw
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  5. D.-F. Tai
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Shih, IL., Chiu, LC., Lai, C. et al. Enzymes catalyzed esterification of N-protected amino acids with secondary alcohols. Biotechnology Letters 19, 857–859 (1997). https://doi.org/10.1023/A:1018329402408

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