Abstract
Invertase synthesis in Schwanniomyces occidentalis is regulated by catabolite repression and is derepressed by raffinose and low concentrations of glucose. Efficiency of a carbon source in derepression of invertase is dependent upon the type of culture medium: either raffinose in a rich medium or a low concentration of glucose in a yeast minimal medium. The kinetics of derepression can be modulated by changing the carbon source. When cells are grown in a rich medium with 0.5% raffinose as the sole carbon source, Schwanniomyces occidentalis secretes 80 times more invertase than Saccharomyces cerevisiae grown in the same conditions. About 50% of the total amount of invertase produced by Schwanniomyces occidentalis is secreted in the extracellular medium in contrast to Saccharomyces cerevisiae where only 6 to 15% of the protein is secreted in the medium.
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Costaglioli, P., Meilhoc, E., Janatova, I. et al. Secretion of invertase from Schwanniomyces occidentalis. Biotechnology Letters 19, 623–627 (1997). https://doi.org/10.1023/A:1018326512461
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DOI: https://doi.org/10.1023/A:1018326512461