Abstract
Biosynthesis of MR-387 by Streptomyces neyagawaensis SL-387 was studied by testing the incorporation of radio-active precursors and the detection of biosynthetic enzyme. L-Phenylalanine, L-valine, L-proline, and acetic acid were efficiently incorporated into MR-387, but phenylethylamine and oxalic acid were not. These results suggest that the (2 S,3 R)-3-amino-2-hydroxy-4-phenylbutanoic acid moiety of MR-387 is synthesized from phenylalanine and acetic acid but not directly via the phenylethylamine. Synthesis of MR-387 is mediated by a peptide synthetase with a novel activity.
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Aoyagi, T., Yoshida, S., Nakamura, S.Y., Shigihara, Y., Hamada, M. and Takeuchi, T. (1990) J. Antihiotics, 43, 143–148.
Banko, G., Wolfe, S. and Demain, A.L. (1986) Biochem. Biophy. Res. Commun., 137, 528–535.
Chung, M.C., Chun, H.K., Han, K.H., Lee, H.J., Lee, C.H. and Kho, Y.H. (1996a) J. Antibiotics, 49, 99–102.
Chung, M.C., Chun, H.K., Lee, H.J., Lee, C.H. and Kho, Y.H. (1995) Kor. J. Appl. Microbiol. Biotechnol., 23, 47–52.
Chung, M.C., Lee, H.J., Chun, H.K., Lee, C.H., Kim, S.I. and Kho, Y.H. (1996b) Biosci. Biotech. Biochem., 60, 898–900.
Hori, K., Kurotsu, T., Kanda, M., Miura, S., Nozoe, A. and Saito, Y. (1978). J. Biochem., 84, 425–434.
Jensen, S.E., Leskiw, B.K., Vining, L.C., Aharoniwitz, Y. and Westlake, D.W.S. (1986) Can. J. Microbiol., 32, 953–958.
Keller, U. (1987) J. Biol. Chem., 262, 5852–5856.
Kleinkauf, H. and von Dohren, H. (1990) Eur. J. Biochem., 192, 1–15.
Krause, M., Marahiel, M.A., von Dohren, H. and Kleinkauf, H. (1985) J. Bacteriol., 162, 1120–1125.
Lindstrom, P. (1986) Biochem. Biophys. Acta, 884, 276–281.
Lipmann, F. (1971) Science, 173, 875–884.
Okuyama, A., Naganawa, H., Harada, S., Aoyagi, T. and Umezawa, H. (1986) Biochem. Int., 12, 627–631.
Schulz, W.A., Gebhardt, R. and Mecke, D. (1985) Eur. J. Biochem., 146, 549–553.
Skarpeid, H.-J., Zimmer, T.-L., Shen, B. and von Dohren, H. (1990) Eur. J. Biochem., 187, 627–633.
Umezawa, H., Aoyagi, T., Snda, H., Hamada, M., and Takeuchi, T. (1976) J. Antihiotics, 29, 97–100.
van Liempt, H., von Dohren, H. and Kleinkauf, H. (1989) J. Biol. Chem., 264, 3680–3684.
Zocher, R., Keller, U. and Kleinkauf, H. (1982) Biochemistry, 21, 43–48.
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Chung, MC., Lee, CH., Lee, HJ. et al. Biosynthesis of peptide inhibitor MR-387 by Streptomyces neyagawaensis. Biotechnology Letters 19, 607–610 (1997). https://doi.org/10.1023/A:1018318210644
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DOI: https://doi.org/10.1023/A:1018318210644