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Measurement of diffusion constants for nucleic acids by NMR

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Abstract

Pulsed field-gradient NMR experiments can be used to measure thediffusion constants of nucleic acids. The diffusion constants measured inthis way for double-helical DNAs of defined length agree well both withtheory and with measurements done using other techniques. When applied toRNAs, this experi- ment easily distinguishes duplex RNAs from RNA hairpinsand thus it can solve one of the perennial problems faced by RNAspectroscopists, i.e. assessing whether their samples are monomeric or not.

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Authors and Affiliations

  1. Department of Chemistry, Yale University, P.O. Box 208107, New Haven, CT, 06520-8107, U.S.A

    Jon Lapham, Jason P. Rife, Peter B. Moore & Donald M. Crothers

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  1. Jon Lapham
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  2. Jason P. Rife
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  3. Peter B. Moore
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Lapham, J., Rife, J.P., Moore, P.B. et al. Measurement of diffusion constants for nucleic acids by NMR. J Biomol NMR 10, 255–262 (1997). https://doi.org/10.1023/A:1018310702909

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