Abstract
RFLP analysis of a PCR amplified 3.2-kbp region of cpDNA bounded by the conserved sequences in rbc L and ORF 106 was performed in eggplant (Solanum melongena), its related Solanum species, S. incanum, S. virginianum (= S. surattense), S. torvum, S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum), S. violaceum (= S. sanitwongsei) and S. mammosum and the reciprocal hybrids between S. aethiopicum (= S. integrifolium) and S. melongena 'Uttara'. The target region of cpDNA was amplified correctly by PCR. The amplified products were digested with each of 10 restriction enzymes (Alu I, Ase I, BamH I, Hinf I, Msp I, Rsa I, ScrF I, Sty I, Taq I and Xba I). Variations of restriction patterns among the species were recognized after digesting the amplified products with each of the seven restriction enzymes, Taq I, Alu I, Rsa I, Sty I, Ase I, Hinf I and Xba I. The restriction patterns divided the examined nine species into the following five clusters, 1) S. melongena and S. incanum, 2) S. virginianum (= S. surattense), 3) S. torvum, 4) S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum) and S. violaceum (= S. sanitwongsei) and 5) S. mammosum. The restriction pattern with Alu I in each of the reciprocal hybrids between S. melongena 'Uttara' and S. aethiopicum (= S. integrifolium) was identical with that of seed parent. The present study demonstrated the availability of the PCR-RFLP analysis of cpDNA for assessing taxonomic relationships and identifying cytoplasmic parentage of interspecific hybrids in eggplant and related Solanum species.
Similar content being viewed by others
References
Arnold, M.L., C.M. Buckner & J.J. Robinson, 1991. Pollen-mediated introgression and hybrid speciation in Louisiana irises. Proc Natl Acad Sci USA 88: 1398-1402.
Badenes, M.L. & D.E. Parfitt, 1995. Phylogenetic relationships of cultivated Prunusspecies from an analysis of chloroplast DNA variation. Theor Appl Genet 90: 1035-1041.
Daunay, M.C., M.H. Chaput, D. Sihachakr, M. Allot, F. Vedel & G. Ducreux, 1993. Production and characterization of fertile somatic hybrids of eggplant (Solanum melongenaL.) with Solanum aethiopicumL. Theor Appl Genet 85: 841-850.
Daunay, M.C., R.N. Lester & H. Laterrot, 1991. The use of wild species for the genetic improvement of brinjal egg-plant (Solanum melongena) and tomato (Lycopersicon esculentum). In: J.G. Hawkes, R.N. Lester, M. Nee & N. Estrada-R (Eds.), Solanaceae III, pp. 369-387. Royal Botanic Gardens, Kew, Richmond (U.K.).
Dubouzet, J.G., T. Etoh, K. Arisumi & T. Yoshitake, 1996. A diagnostic test to confirm interspecific Alliumhybrids using random amplified polymorphic DNA from crude leaf DNA extracts. J Jpn Soc Hort Sci 65: 321-326.
Guri, A. & K.C. Sink, 1988. Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum. Theor Appl Genet 76: 490-496.
Isshiki, S., H. Okubo & K. Fujieda, 1994. Phylogeny of eggplant and related Solanumspecies constructed by allozyme variation. Sci Hortic 59: 171-176.
Liston, A., 1992. Variation in the chloroplast genes rpoC1 and rpoC2 of the genus Astragalus(Fabaceae): evidence from restriction site mapping of a PCR-amplified fragment. Amer J Bot 79: 953-961.
Liston, A., L.H. Rieseberg & M.A. Hanson, 1992. Geographic partitioning of chloroplast DNA variation in the genus Datisca(Datiscaseae). Pl Syst Evol 181: 121-132.
Nei, M. & W. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76: 5269-5273.
Ogihara, Y., T. Terachi & T. Sasakuma, 1991. Molecular analysis of the hot spot region related to length mutations in wheat chloroplast DNAs. I. Nucleotide divergence of genes and inter-genic spacer regions located in the hot spot region. Genetics 129: 873-884.
Perez de la Rosa, J., S.A. Harris & A. Farjon, 1995. Noncoding chloroplast DNA variation in Mexican pines. Theor Appl Genet 91: 1101-1106.
Reboud, X. & C. Zeyl, 1994. Organelle inheritance in plants. Heredity 72: 132-140.
Sakata, Y. & R.N. Lester, 1994. Chloroplast DNA diversity in eggplant (Solanum melongena) and its related species S. incanumand S. marginatum. Euphytica 80: 1-4.
Sakata, Y., T. Nishio & P.J. Matthews, 1991. Chloroplast DNA analysis of eggplant (Solanum melongena) and related species for their taxonomic affinity. Euphytica 55: 21-26.
Yanai, H. & H. Takagi, 1986. The Handbook of Multivariate Analysis. Gendaisugakusha, Kyoto.
Yonemori, K., D.E. Parfitt, S. Kanzaki, A. Sugiura, N. Utsunomiya & S. Subhadrabandhu, 1996. RFLP analysis of an amplified region of cpDNA for phylogeny of the genus Diospyros. J Jpn Soc Hort Sci 64: 771-777.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Isshiki, S., Uchiyama, T., Tashiro, Y. et al. RFLP analysis of a PCR amplified region of chloroplast DNA in eggplant and related Solanum species. Euphytica 102, 295–299 (1998). https://doi.org/10.1023/A:1018304308608
Issue Date:
DOI: https://doi.org/10.1023/A:1018304308608