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Preparation and Evaluation of 125I-Aflatoxin B1

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Abstract

Aflatoxin B1 (AfB1), present in fungus infested crops is highly carcinogenic and is measured by immunoassays. 125I labeled aflatoxin B1 is a key reagent for development of radioimmunoassay (RIA) which exhibits less interference and better sensitivity than other immunoassays. Since AfB1 lacks suitable functional groups for radiolabeling, an oxime derivative of AfB1 was synthesised and evaluated by UV-spectrophotometry and 1H NMR spectroscopy. 125I-histamine was conjugated to AfB1 oxime by mixed anhydride method and purified by solvent extraction followed by TLC. The tracer obtained was immunoreactive, stable as ethanolic solution and could be used in RIA.

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References

  1. K. K. Maggon,S. K. Gupta, Venketasubramanian, Bacteriol. Rev., 41 (1977) 822.

    Google Scholar 

  2. K. K. Sinha,D. D. Bhatnagar (Eds) Mycotoxins in Agriculture and Food Safety, Marcel Dekker, Inc. New York, 1998.

    Google Scholar 

  3. J. D. Groopman,L. G. Cain,T. W. Kensler, CRC Crit. Rev. Toxicol., 19 (1988) 113.

    Google Scholar 

  4. G. N. Wogan, Methods for Detecting DNA Damaging Agents in Humans: Applications in Cancer Epidemiology and Prevention, H. Bartsch,K. Hemminki andI. K. O'Neill (Eds), 32–51, International Agency of Research on Cancer, 1988, p. 32.

  5. A. K. Sharma,M. R. A. Pillai, Encyclopaedia of Food Technology, Academic Press, New York, 1999, p. 1532.

    Google Scholar 

  6. J. W. Dorner, Handbook of Food Analysis, Vol. 2, Leo M. L. Nollet (Ed.), Marcel Dekker Inc., New York, 1996.

    Google Scholar 

  7. M. E. Stack, Handbook of Th in Layer Chromatography, J. SHERMA,B. Fried (Eds), Marcel Dekker Inc., 1996, p. 1033.

  8. U. Samarajeeva,C. I. Wei,T. S. Huang,M. R. Marshal, CRC Crit. Revs in Food Science and Nutrition, 29 (1991) 403.

    Google Scholar 

  9. J. E. T. Corrier,W. M. Hunter, Meth. Enzymol., 73 (1981) 79.

    Google Scholar 

  10. A. Korde,M. Ventatesh,R. Lal,M. R. A. Pillai, J. Radioanal. Nucl. Chem., 206 (1996) 219.

    Google Scholar 

  11. F. S. Chu,I. Ueno, Appl. Environ. Microbiol., 33 (1977) 1125.

    PubMed  Google Scholar 

  12. A. E. Bolter, Immunoassays for 80's., A. Voller,A. Bartlett andD. Bidwell (Eds), University Park Press, Baltimore, USA, 1981.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Radiopharmaceutical Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India

    A. Korde, S. Banerjeė, M.R.A. Pillai & M. Venkatesh

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  1. A. Korde
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  2. S. Banerjeė
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  3. M.R.A. Pillai
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  4. M. Venkatesh
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Korde, A., Banerjeė, S., Pillai, M. et al. Preparation and Evaluation of 125I-Aflatoxin B1 . Journal of Radioanalytical and Nuclear Chemistry 250, 231–237 (2001). https://doi.org/10.1023/A:1017998202131

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