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Overexpression of the nucleolar protein nucleophosmin/B23 in collagen lattice-cultured fibroblasts: Potential role in the control of protein synthesis

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Abstract

Fibroblasts cultivated in tridimensional collagen lattices exhibit a downregulation of protein synthesis, related to decreased ribosomal RNA (rRNA) content and half life, when compared to monolayer cultivated cells. The involvement in this process of nucleophosmin/B23, a nucleolar phosphoprotein with ribonuclease properties, was checked. We compared production of nucleophosmin/B23 in monolayer and collagen lattice cultured fibroblasts. A significant increase of nucleophosmin/B23 mRNA levels was noticed in lattice-cultured fibroblasts vs monolayers (+154%, p < 0.05). A concomitant enhancement of nucleolar nucleophosmin/B23 content was found (+112%, p < 0.001). Simultaneously, ribonuclease activity contained in nucleolar extracts from collagen lattice-cultured fibroblasts was significantly increased (+54%, p < 0.01). These data demonstrate that extracellular collagen matrix induces the overexpression of nucleophosmin/B23, and suggest that the regulation of protein syntheses in collagen lattice cultures may be explained, at least partly, by an increased degradation of neosynthesized rRNAs dependent on nucleophosmin.

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Authors and Affiliations

  1. Laboratoire de Biochimie et Biologie Moléculaire, Faculté de Médecine, 51 rue Cognacq-Jay, 51095, Reims cedex, France

    Fabrice Lefèvre, Roselyne Garnotel, Nadine Georges & Philippe Gillery

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  1. Fabrice Lefèvre
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  2. Roselyne Garnotel
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  3. Nadine Georges
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  4. Philippe Gillery
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Correspondence to Philippe Gillery.

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Lefèvre, F., Garnotel, R., Georges, N. et al. Overexpression of the nucleolar protein nucleophosmin/B23 in collagen lattice-cultured fibroblasts: Potential role in the control of protein synthesis. Mol Cell Biochem 229, 45–50 (2002). https://doi.org/10.1023/A:1017995111734

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