Abstract
Two tomato (Lycopersicon esculentum L.) cultivars: Robin (tolerant) and Roma (sensitive to heat stress) were studied. Chlorophyll fluorescence induction parameters (Fv/Fp, Amax, and Rfd) at 25 °C showed that the PS2 activity was similar for both cultivars. The parameters, measured at 38 °C, decreased in both cultivars, but more in cv. Roma. Exogenous application of 4 mM spermidine improved the plant heat-resistance in both cultivars, and especially in cv. Roma. Analysis of chlorophyll fluorescence changes during linear increase in temperature showed that cv. Robin plants have higher ability to hardening and higher resistance to thermal damage of the pigment-protein complexes structure and the activity of PS2 than cv. Roma.
Similar content being viewed by others
Reference
Besford, R.T., Richardson, C.M., Capell, T., Tiburcio, A.F.: Effect of polyamine on stabilization of molecular complexes in thylakoid membranes on osmotically stressed oat leaves.-Planta 189: 201–206, 1993.
Bilger, H.W., Schreiber, U., Lange, O.L.: Determination of leaf heat resistance: comparative investigation of chlorophyll fluorescence changes and tissue necrosis methods.-Oecologia 63: 256–262, 1984.
Bouchereau, A., Azis, A., Larher, F., Martin-Tanguy J.: Polyamines and environmental challenges: recent development.-Plant Sci. 140: 103–125, 1999.
Bukhov, N.G., Dzhibladze, T.G., Karapetyan, N.V.: [Aftereffects of high temperatures on the kinetics of variable and delayed fluorescence in leaves.]-Fiziol. Rast. 34: 435–444, 1987. [In Russ.]
Cohen, A.S., Popovic, R.B., Zalik, S.: Effects of polyamines on chlorophyll and protein content, photochemical activity and chloroplast ultrastructure of barley leaf discs during senescence.-Plant Physiol. 64: 717–720, 1979.
Daniel, E.: The temperature dependence of photoinhibition in leaves of Phaseolus vulgaris (L.).-Plant Sci. 124: 1–8, 1997.
Drolet, G., Dumbroff, E.B., Legge, R.L., Thompson, J.E.: Radical scavenging properties of polyamines.-Phytochemistry 25: 367–371, 1986.
Edreva, A., Yordanov, I., Kardijeva, R., Gesheva, E.: Heat shock responses of bean plants: involvement of free radicals, antioxidants and free radical/active oxygen scavenging systems.-Biol. Plant. 41: 185–191, 1998.
Floryszak-Wieczorek, J., Grabikowski, E., Kubiś, J., Krzywański, Z.: The effect of spermidine on lipid peroxidation in wheat leaves during water stress.-Acta Physiol. Plant. 14: 3–10, 1992a.
Floryszak-Wieczorek, J., Murkowski, A., Kubiś, J., Krzywański, Z.: The effect of spermidine on chlorophyll luminescence in wheat leaves under water stress.-Acta Physiol. Plant. 14: 119–126, 1992b.
Havaux, M.: Stress tolerance of photosystem II in vivo.-Plant Physiol. 100: 424–432, 1992.
Kislyuk, L.M., Bubolo, L.S., Paleeva, T.V.: [Heat shock effects on primary thermostability and reparability of photosynthesis and on the ultrastructure of mesophyll cells in strawberry Fragaria vesca L.]-Fiziol. Rast. 39: 939–948, 1992. [In Russ.]
Lavorel, J., Breton, J., Lutz, M.: Methodological principles of measurements of light emitted by photosynthetic systems.-In: Govindjee Amesz, J., Fork, D.C. (ed.): Light Emission by Plants and Bacteria. Pp. 57–98. Academic Press, Orlando 1986.
Lichtenthaler, H., Buschmann, C., Rinderle, U., Schmuck, G.: Application of chlorophyll fluorescence in ecophysiology.-Radiat. Environ. Biophys. 25: 297–308, 1986.
Lichtenthaler, H., Rinderle, U.: The role of chlorophyll fluorescence in the detection of stress conditions in plants.-Crit. Rev. anal. Chem. 19: 29–85, 1988.
Porankiewicz, J., Gwóźdź, E.A.: [Heat shock proteins and their role in plant cell.]-Post. Biol. Komórki 20(2): 155–170, 1993. [In Polish.]
Pospíšil, P., Skotnica, J., Nauš, J.: Low and high temperature dependence of minimum Fo and maximum Fm chlorophyll fluorescence in vivo.-Biochim. biophys. Acta 1363: 95–99, 1998.
Roy, M., Gosh, B. Polyamines, both common and uncommon, under heat stress in rice (Oryza sativa) callus.-Physiol. Plant. 98: 196–200, 1996.
Schuber, F.: Influence of polyamines on membrane function.-Biochem. J. 260: 1–10, 1989.
Seemann, J.R., Berry, J.A., Downton, W.J.S.: Photosynthetic response and adaptation to high temperature in desert plants. A comparison of gas exchange and fluorescence methods for studies of thermal tolerance.-Plant Physiol. 75: 364–368, 1984.
Starck, Z., Wazynska, Z., Kucewicz, O.: Comparative effects of heat stress on photosynthesis and chloroplast ultrastructure in tomato plants with source-sink modulated by growth regulators.-Acta Physiol. Plant. 16: 125–133, 1993.
Terzaghi, W.B., Fork, D.C., Berry, J.A., Field, C.B.: Low and high temperature limits to PSII.-Plant Physiol. 91: 1494–1500, 1989.
Thebut, R., Santarius, K.A.: Effects of high temperature stress on various biomembranes of leaf cell in situ and in vitro.-Plant Physiol. 70: 200–205, 1982.
Vierling, E.: The roles of heat shock protein in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 579–620, 1991.
Volger, H., Santarius, K.A.: Release of membrane proteins in relation to heat injury of spinach chloroplast.-Physiol. Plant. 51: 195–200, 1981.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murkowski, A. Heat Stress and Spermidine: Effect on Chlorophyll Fluorescence in Tomato Plants. Biologia Plantarum 44, 53–57 (2001). https://doi.org/10.1023/A:1017966203859
Issue Date:
DOI: https://doi.org/10.1023/A:1017966203859