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Combining Drosophila melanogaster somatic-mutation-recombination and electron-spin-resonance-spectroscopy data to interpret epidemiologic observations on chromium carcinogenicity

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Abstract

Lung cancers are significantly increased among workers exposed to chromate (Crc6+, Cr3+), chromium pigments (Cr6+) and chromium plating (Cr6+). Chromiun lung burdens and cancer risk increase proportionately with duration of employment at long latencies. However, this epidemiologic information alone is insufficient in determining whether Cr6+ or Cr3+ are equally important in causing cancer. We have attempted to combine epidemiologic data with data from the Drosophila melanogaster somatic-mutation-recombination-test and from the in vitro electron-spin-resonance spectroscopy study to demonstrate that following somatic recombination plays a more important role than somatic mutation in chromium carcinogenesis. Cr4+ is more important than Cr5+or Cr6+ in inducing somatic recombination while Cr6+ produces more and bigger clones than Cr4+ in somatic mutation. Cr3+ produces negative results in this fruit-fly wing-spot-assay. When the larvae and flies exposed to Cr6+ and Cr4+ are examined by ESR, only Cr5+ and Cr3+ are found. Thermodynamic parameters ΔE, ΔH, and ΔS are also estimated from these latter experiments to explain the relative importance of Cr6+, Cr4+, Cr3+ in chromium carcinogenesis among exposed industrial workers.

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Authors and Affiliations

  1. Department of Biological Sciences, Illinois State University, Normal, IL, 61701, USA

    Alan J. Katz

  2. Office Research and Development, National Center for Environment Assessment, Washington, DC, 20460, USA

    Arthur Chiu

  3. United States Environmental Protection Agency, Office of Pollution Prevention and Toxics, Washington, DC, 20460, USA

    Jefferson Beaubier

  4. Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, WV, 26505, USA E-mail

    Xianglin Shi

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  1. Alan J. Katz
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  2. Arthur Chiu
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  4. Xianglin Shi
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Katz, A.J., Chiu, A., Beaubier, J. et al. Combining Drosophila melanogaster somatic-mutation-recombination and electron-spin-resonance-spectroscopy data to interpret epidemiologic observations on chromium carcinogenicity. Mol Cell Biochem 222, 61–68 (2001). https://doi.org/10.1023/A:1017959222379

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