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Lactobacillus plantarum 299v Inhibits Escherichia coli-Induced Intestinal Permeability


The purpose of this work was to investigate whether a probiotic bacterium, Lactobacillus plantarum 299v, could affect Escherichia coli-induced passage of mannitol across the intestinal wall. Sprague-Dawley rats were pretreated for one week by either tube feeding with L. plantarum 299v twice daily, free access to L. plantarum 299v by adding the bacterium in the drinking water, or negative control receiving regular feeding. Intestinal segments were mounted in Ussing chambers and the mucosa was exposed to control medium, E. coli, and L. plantarum 299v (alone or together). [14C]Mannitol was added as a marker of intestinal permeability and samples were taken from the serosal side. E. coli exposure induced a 53% increase in mannitol passage across the intestinal wall (P < 0.05). One week of pretreatment with L. plantarum 299v in the drinking water abolished the E. coli-induced increase in permeability. Tube feeding for one week or short-term addition of L. plantarum 299v in the Ussing chambers had no effect on the permeability provoked by E. coli challenge. Notably, L. plantarum 299v itself did not change the intestinal passage of mannitol. These data demonstrate that pretreatment with L. plantarum 299v, which is a probiotic bacterium, protects against E. coli-induced increase in intestinal permeability, and that L. plantarum 299v alone has no influence on the intestinal permeability. Thus, this study supports the concept that probiotics may exert beneficial effects in the gastrointestinal tract.

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  1. 1.

    Berg RD: Bacterial translocation from the gastrointestinal tract. Adv Exp Med Biol 473:11–30, 1999

    Google Scholar 

  2. 2.

    Pape HC, Dwenger A, Regel G, Auf' m'Kolck M, Gollub F, Wisner D, Sturm JA, Tscherne H: Increased gut permeability after multiple trauma. Br J Surg 81:850–852, 1994

    Google Scholar 

  3. 3.

    LeVoyer T, Cioffi WGJ, Pratt L: Alterations in intestinal permeability after thermal injury. Arch Surg 127:26–29, 1992

    Google Scholar 

  4. 4.

    Johnston JD, Harvey CJ, Menzies IS: Gastrointestinal permeability and absorptive capacity in sepsis. Crit Care Med 24:1144–1149, 1996

    Google Scholar 

  5. 5.

    Olaison G, Sjöberg ÅK, Tagesson C: Abnormal intestinal permeability in Crohn's disease. A possible pathogenic factor. Scand J Gastroenterol 25:321–328, 1990

    Google Scholar 

  6. 6.

    Nejdfors P, Wang Q, Ekelund M, Weström BR, Jansson O, Jeppsson B: Increased colonic permeability in patients with ulcerative colitis: An in vitro study. Scan J Gastroenterol 33:749–753, 1998

    Google Scholar 

  7. 7.

    Söderholm JD, Peterson KH, Olaison G, Franzen LE, Weström BR, Magnusson KE, Sjödahl R: Epithelial permeability to proteins in the noninflamed ileum of Crohn's disease? Gastroenterology 117:65–72, 1999

    Google Scholar 

  8. 8.

    Kops SK, Lowe DK, Bement WM, West AB: Migration of Salmonella typhi through intestinal epithelial monolayers: An in vitro study. Microbiol Immunol 40:799–811, 1996

    Google Scholar 

  9. 9.

    Spitz J, Yuhan R, Koutsouris A, Blatt C, Alverdy J, Hecht G: Enteropathogenic Escherichia coli adherence to intestinal epithelial monolayers diminshes barrier function. Am J Physiol 268:G374–G379, 1995

    Google Scholar 

  10. 10.

    Siitonen S, Vapaatalo H, Salminen S, Gordin A, Saxelin M, Wikberg R, Kirkkola AL: Effect of Lactobacillus GG yoghurt in prevention of antibiotic associated diarrhoea. Ann Med 22:57–59, 1990

    Google Scholar 

  11. 11.

    Majamaa H, Isolauri E, Saxelin M, Vesikari T: Lactic acid bacteria in the treatment of acute rotavirus gastroenteritis. J Pediatr Gastroenterol Nutr 20:333–338, 1995

    Google Scholar 

  12. 12.

    Mao Y, Nobaek S, Kasravi B, Adawi D, Stenram U, Molin G, Jeppsson B: The effects of Lactobacillus strains and oat fiber on methotrexate-induced enterocolitis in rats. Gastroenterology 111:334–344, 1996

    Google Scholar 

  13. 13.

    Adawi D, Kasravi B, Molin G, Jeppsson B: Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat. Hepatology 25:642–647, 1997

    Google Scholar 

  14. 14.

    Madsen KL, Doyle JS, Jewell LD, Tavernini MM, Fedorak RN: Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. Gastroenterology 116:1107–1114, 1999

    Google Scholar 

  15. 15.

    Holzapfel WH, Haberer P, Snel J, Schillinger U, Huis int Veld JJJ: Overview of gut flora and probiotics. Int J Food Microbiol 41:85–101, 1998

    Google Scholar 

  16. 16.

    Adawi D, Molin G, Ahrné S, Jeppsson B: Modulation of the colonic bacterial flora affects differently bacterial translocation and liver injury in an acute liver injury model. Microb Ecol Health Dis 11:47–54, 1999

    Google Scholar 

  17. 17.

    Mao Y, Yu J-L, Ljungh Å, Molin G, Jeppsson B: Intestinal immune response to oral administration of Lactobacillus reuteri R2LC, Lactobacillus plantarum DSM 9843, pectin and oatbase on methotrexate-induced enterocolitis in rats. Microb Ecol Health Dis 9:261–270, 1996

    Google Scholar 

  18. 18.

    Johansson M-L, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S: Administration of different Lactobacillus strains in fermented oatmeal soup: In vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl Environ Microbiol 5915–20, 1993

  19. 19.

    Efthymoiu C, Hansen CA: An antigenic analysis of Lactobacillus acidophilus. J Infect Dis 110:258–267, 1962

    Google Scholar 

  20. 20.

    Ryan CM, Yarmush ML, Burke JF, Tompkins RG: Increased gut permeability early after burns correlates with the extent of burn injury. Crit Care Med 20:1508–1512, 1992

    Google Scholar 

  21. 21.

    Prescott L M, Harley JP, Klein DA: Microbiology. Dubuque, Iowa, W C Brown Publishers

  22. 22.

    MacFie J, O'Boyle CJ, Mitchell CJ, Buckley PM, Johnstone D, Sudworth P: Gut origin of sepsis: A prospective study investigating associations between bacterial translocation, gastric microflora and septic morbidity. Gut 45:223–228, 1999

    Google Scholar 

  23. 23.

    Sedman PC, MacFie J, Sagar P, Mitchell ChJ, May J, Mancey-Jones B, Johnstone D: The prevalence of gut translocation in humans. Gastroenterology 107:643–649, 1994

    Google Scholar 

  24. 24.

    Krugliak P, Hollander D, Schlaepfer CC, Nguyen H, Ma TY: Mechanisms and sites of mannitol permeability of small and large intestine in the rat. Dig Dis Sci 39:796–801, 1994

    Google Scholar 

  25. 25.

    Herias MV, Hessle C, Telemo E, Midtvedt T, Hansson LÅ, Wold AE: Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats. Clin Exp Immunol 116:283–290, 1999

    Google Scholar 

  26. 26.

    Livesy G, Elia M: Short-chain fatty acids as an energy source in the colon: metabolism and clinical implications. In Physiological and Clinical Aspects of Short-Chain Fatty Acids. JH Cummings, JC Rombeau, T Sakata (eds). Cambridge, Cambridge University Press, 1995, pp 427–481

    Google Scholar 

  27. 27.

    Johansson ML, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S: Administration of different Lactobacillus strains in fermented oatmeal soup; in vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl Environ Microbiol 59:15–20, 1993

    Google Scholar 

  28. 28.

    Söderholm JD, Hedman L, Artursson P, Franzén L, Larsson J, Pantzar N, Permert J, Olaison G: Integrity and metabolism of human ileal mucosa in vitro in the Ussing chamber. Acta Physiol Scand 162:47–56, 1998

    Google Scholar 

  29. 29.

    Armstrong WA: Cellular mechanisms of ion transport in the small intestine. In Physiology of the Gastrointestinal Tract. LR Johnson (ed). New York, Raven Press, 1987, pp 1251–1265

    Google Scholar 

  30. 30.

    Kurkchubasche AG, Cardona M, Watkins SC, Smith SD, Albanese CT, Simmons RL, Rowe SC, Ford HR: Transmucosal passage of bacteria across rat intestinal epithelium in the Ussing chamber: Effect of nutritional factors and bacterial virulence. Shock 9:121–127, 1998

    Google Scholar 

  31. 31.

    Albanese CT, Cardona M, Smith SD, Watkins SC, Kurkchubasche AG, Ulman I, Simmons RL, Rowe MI: Role of intestinal mucus in transepithelial passage of bacteria across the intact ileum in vitro. Surgery 116:76–82, 1994

    Google Scholar 

  32. 32.

    Polentarutti BI, Peterson AL, Sjöberg ÅK, Anderberg EKI, Utter LM, Ungell A-LB: Evaluation of viability of excised rat intestinal segments in the Ussing chamber: Investigation of morphology, electrical parameters and permeability characteristics. Pharmacol Res 16:446–454, 1999

    Google Scholar 

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Mangell, P., Nejdfors, P., Wang, M. et al. Lactobacillus plantarum 299v Inhibits Escherichia coli-Induced Intestinal Permeability. Dig Dis Sci 47, 511–516 (2002).

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  • Lactobacillus plantarum
  • Escherichia coli
  • intestinal mucosa
  • permeability
  • probiotic bacteria
  • Ussing chamber