Abstract
Random amplified polymorphic DNA fingerprinting was performed on single females of root-knot nematodes, Meloidogyne spp., using a new procedure for DNA isolation. One-fourth of the total DNA amount isolated from a single female proved to be sufficient as a template in a polymerase chain reaction. Electrophoretic patterns of the amplified fragments were reproducible between replicates from a single female or sister females from the same progeny, and identical to those obtained with genomic DNA purified from a large number of nematodes. Moreover, a comparative analysis over three successive generations showed stability of the amplification patterns, thus demonstrating the utility of this procedure for epidemiological and ecological studies on root-knot nematodes.
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Randig, O., Leroy, F. & Castagnone-Sereno, P. RAPD Characterization of Single Females of the Root-knot Nematodes, Meloidogyne Spp.. European Journal of Plant Pathology 107, 639–643 (2001). https://doi.org/10.1023/A:1017936527466
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DOI: https://doi.org/10.1023/A:1017936527466