Abstract
Two types are known in the Kanzawa spider mite, Tetranychus kanzawai (K and T; see Gotoh et al., 1999), which differ in host range and have a unidirectional incompatibility. Prior to DNA analyzes, crossing between females of a known K type and males of each of 17 strains collected in Japan showed that six of the strains were of the K type, five were the T type and the rest consisted of a mixture of the two types. In order to elucidate the genetic diversity and phylogenetic relationship of T. kanzawai in Japan, we analyzed the DNA sequences of two regions – the internal transcribed spacer 1 (ITS1) of the nuclear ribosomal DNA (rDNA) and a fragment of the cytochrome oxidase subunit I gene (COI) of mitochondrial DNA – using 11 strains (six K-type strains and five T-type strains). Base substitutions were detected on 25 sites of COI (375 bp) and 19 sites of ITS1 (486 bp), resulting in eight and 17 haplotypes, respectively. The phylogenetic trees constructed using the DNA sequences failed to clearly distinguish between the two types. The results suggested that the T type was derived from the K type.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breeuwer, J.A.J. andJacobs, G. 1996. Wolbachia: intracellular manipulators of mite reproduction. Exp. Appl. Acarol. 20: 421–434.
Brower, A.V.Z. andDeSalle, R. 1994. Practical and theoretical considerations for choice of a DNA sequence region in insect molecular systematics, with a short review of published studies using nuclear regions. A nn. Entomol. Soc. Am. 87: 702–716.
Brower, A.V.Z.,DeSalle, R. andVogler, A. 1996. Gene trees, species trees, and systematics: acladistic perspective. Annu. Rev. Ecol. Syst. 27: 423–450.
Bush, G.L. 1969. Sympatric host race formation and speciation in frugivorous flies of the genus Rhagoletis (Diptera, Tephritidae). Evolution 23: 237–251.
Bush, G.L. andSmith, J.J., 1997. The sympatric origin of phytophagous insects. In: Vertical Food Web Interactions: Evolutionary Patterns and Driving Forces, K. Dettner,G. Bauer andW. Völkel (eds), pp. 3–19. Springer-Verlag, Berlin.
Ehara, S. 1999. Revision of the spider mite family Tetranychidae of Japan (Acari, Prostigmata). Species Diver. 4: 63–141.
Gaillard, C. andStrauss, F. 1990. Ethanol precipitation of DNA with linear polyacrylamide as carrier. Nucl. Acids Res. 19: 378.
Gomi, K. andGotoh, T. 1996. Host plant preference and genetic compatibility of the kanzawa spider mite, Tetranychus kanzawai Kishida (Acari: Tetranychidae). Appl. Entomol. Zool. 31: 417–425.
Gomi, K.,Gotoh, T. andNoda, H. 1997. Wolbachia having no effect on reproductive incompatibility in Tetranychus kanzawai Kishida (Acari: Tetranychidae). Appl. Entomol. Zool. 32: 485–490.
Gotoh, T.,Gomi, K. andNagata, T. 1999. Incompatibility and host plant differences among populations of Tetranychus kanzawai Kishida (Acari: Tetranychidae). Appl. Entomol. Zool. 34: 551–561.
Johanowicz, D.L. andHoy, M.A. 1996. Wolbachia in a predator-prey system: 16S ribosomal DNA analysis of two phytoseiids (Acari: Phytoseiidae) and their prey (Acari: Tetranychidae). Ann. Entomol. Soc. Am. 89: 435–441.
Lincoln, S.E.,Daly, M.J. andLander, E.S. 1991. PRIMER: A computer program for automatically selecting PCR primers. MIT Center for Genome Research and Whitehead Institute for Biomedical Research, Nine Cambridge Center. Cambridge, Massachusetts 02142.
Navajas, M.,Gutierrez, J.,Lagnel, J. andBoursot, P. 1996. Mitochondrial cytochrome oxidase I in tetranychid mites: a comparison between molecular phylogeny and changes of morphological and life history traits. Bull. Entomol. Res. 86: 407–417.
Navajas, M.,Gutierrez, J. andGotoh, T. 1997. Convergence of molecular and morphological data reveals phylogenetic information on Tetranychus species and allows the restoration of the genus Amphitetranychus (Acari: Tetranychidae). Bull. Entomol. Res. 87: 283–288.
Nei, M. andLi, W.-H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 76: 5269–5273.
O'Neill, S.L.,Giordano, R.,Colbert, A.M.E.,Karr, T.L. andRobertson, H.M. 1992. 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc. Natl. Acad. Sci. USA. 89: 2699–2702.
Simon, C.,Frati, F.,Beckenbach, A.,Crespi, B.,Liu, H. andFlook, P. 1994. E volution, weighting, and phylogenetic utility of mitochondrial gene sequences and a comparison of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651–701.
Smith, J.J. andBush, G. L. 1997. Phylogeny of the genus Rhagoletis (Diptera: Tephritidae) inferred from DNA sequences of mitochondrial cytochrome oxidase II. Mol. Phylogenet. Evol. 7: 33–43.
Strimmer, K. andHaeseler, A.v. 1996. Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Mol. Biol. Evol. 13: 964–969.
Tamura, K. andNei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10: 512–526.
Tautz, D.,Hancock, J.M.,Webb, D.A.,Tautz, C. andDover, G.A. 1988. Complete sequences of the rRNA genes of Drosophila melanogaster. Mol. Biol. Evol. 5: 366–376.
Thompson, J.D.,Higgins, D.G. andGibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673–4680.
Wesson, D.M. andCollins, F.H. 1992. Sequence and secondary structure of 5.8S rRNA in the tick, Ixodes scapularis. Nucl. Acids Res. 20: 1139.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hinomoto, N., Takafuji, A. Genetic diversity and phylogeny of the Kanzawa spider mite, Tetranychus kanzawai, in Japan. Exp Appl Acarol 25, 355–370 (2001). https://doi.org/10.1023/A:1017934218898
Issue Date:
DOI: https://doi.org/10.1023/A:1017934218898