Abstract
Mitochondria isolated from root tissue of maize plants grown on a modified Knop solution containing 10.9 mM nitrate ± 7.2 mM ammonium were purified on the discontinuous Percoll density gradient with polyvinylpyrrolidone (PVP) added. The presence of PVP allowed separation of several mitochondrial fractions of a different density. Contrary to mitochondria isolated from plants grown in the presence of nitrate alone, revealing only two fractions, the mitochondria from NH4 +/NO3 −-plants were distributed in four fractions. Total amount of mitochondria, as well as specific activities of some nitrogen metabolism enzymes and tricarboxylic acid (TCA) cycle enzymes of all mitochondrial fractions, and respiratory activities of two lower density fractions isolated from plants grown on mixed nitrogen were higher in comparison to mitochondria from nitrate-grown plants.
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Aebi, H.: Catalase.-In: Bergmeyer, H.U. (ed.): Methods in Enzymatic Analysis. Vol. 2. Pp. 673–678. Verlag Chemie, Weinheim 1974.
Bergmeyer, H.U., Bernt, E.: Colorimetric assay of Tonhazy.-In: Bergmeyer, H.U. (ed.): Methods in Enzymatic Analysis. Vol. 2. Pp. 764–767. Verlag Chemie, Weinheim 1974.
Bulen, W.A.: The isolation and characterization of glutamate dehydrogenase from corn leaves.-Arch. Biochem. Biophys. 62: 402–414, 1956.
Couée, I., Jan, M., Carde, J.-P., Brouquisse, R., Raymond, P., Pradet, A.: Effects of glucose starvation on mitochondrial subpopulation in the meristematic and submeristematic regions of maize root.-Plant Physiol. 100: 1891–1900, 1992.
Day, D.A., Neuburger, M., Douce, R.: Biochemical characterization of chlorophyll-free mitochondria from pea leaves.-Aust. J. Plant Physiol. 12: 219–228, 1985.
Douce, R., Christensen, E.L., Bonner, W.D.: Preparation of intact plant mitochondria.-Biochim. biophys. Acta 275: 148–160, 1972.
Estabrook, R.W.: Mitochondrial respiratory control and the polarographic measurement of ADP/O ratios.-Methods Enzymol. 10: 41–47, 1967.
Hadži-Tašković Šukalović, V., Vuletić, M.: Properties of maize root mitochondria from plants grown on different nitrogen sources.-J. Plant Physiol. 153: 67–73, 1998.
Hayes, K.M., Luethy, H.M., Elthon, E.J.: Mitochondrial malate dehydrogenase from corn.-Plant Physiol. 97: 1381–1387, 1991.
King, T.E.: Preparation of succinate dehydrogenase and reconstitution of succinate oxidase.-Methods Enzymol. 10: 322–331, 1967.
Lambers, H., Day, D.A., Azcón-Bieto, J.: Cyanide-resistant respiration in roots and leaves. Measurements with intact tissues and isolated mitochondria.-Physiol. Plant. 58: 148–154, 1983.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.
Rasmusson, A.G., Møller, J.M.: NADP-utilizing enzymes in the matrix of plant mitochondria.-Plant Physiol. 94: 1012–1018, 1990.
Saviani, E.E., da Silva, A., Jr., Martins, I.S.: Role of F1F0-ATP synthase and uncoupling protein in mitochondrial respiratory uncoupling of ripened avocado fruits.-Plant Physiol. Biochem. 36: 575–582, 1998.
Schwitzguebel, J.-P., Siegenthaler, P.-A.: Purification of peroxisomes and mitochondria from spinach leaf by Percoll gradient.-Plant Physiol. 75: 670–674, 1984.
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Hadži-Tašković Šukalović, V., Vuletić, M. Heterogeneity of Maize Root Mitochondria from Plants Grown in the Presence of Ammonium. Biologia Plantarum 44, 101–104 (2001). https://doi.org/10.1023/A:1017930623423
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DOI: https://doi.org/10.1023/A:1017930623423