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Genetica

, Volume 110, Issue 2, pp 151–155 | Cite as

18S-28S rDNA is interspersed throughout heterochromatin in the millepede Enologus oxypygum (Diplopoda)

  • R. Vitturi
  • M.S. Colomba
  • M. Mandrioli
  • A.M. Pirrone
  • G. Grispo
  • R. Barbieri
Article

Abstract

In the present study, somatic metaphase chromosomes of the millipede Enologus oxypygum (Diplopoda: Julida) were hybridized in situ with a sea urchin (Echinodermata) ribosomal probe (prRl4) in order to map major RNA genes (rDNA). Chromosomal preparations were also silver stained (Ag-NOR) to evaluate the rDNA transcriptional activity. Our results indicate that RNA genes are throughout heterochromatin in eight chromosomes involving 1/4 of the total heterochromatin which, in this species, is about 67% of the total DNA. Ag-NOR and FISH patterns were not coincident.

chromosomes diplopoda Enologus oxypygum FISH heterochromatin invertebrate rDNA 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • R. Vitturi
    • 1
  • M.S. Colomba
    • 2
  • M. Mandrioli
    • 3
  • A.M. Pirrone
    • 4
  • G. Grispo
    • 1
  • R. Barbieri
    • 4
  1. 1.Dipartimento di Biologia AnimaleUniversità di PalermoPalermoItaly
  2. 2.Facoltà di Scienze AmbientaliUniversità di Urbino, Localita CrocicchiaUrbinoItaly
  3. 3.Dipartimento di Biologia AnimaleUniversità di Modena e Reggio EmiliaModenaItaly
  4. 4.Dipartimento di Biologia Cellulare e dello Sviluppo ‘A. Monroy’Università di Palermo, Parco d’PalermoItaly

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