Skip to main content
SpringerLink
Log in

Characterization and chromosome location of satellite DNA in the leaf beetle Chrysolina americana (Coleoptera, Chrysomelidae)

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

This paper is the first record of the satellite DNA of the specialized phytophagous genus Chrysolina. The satellite DNA of Chrysolina americana is organized in a tandem repeat of monomers 189 bp long, has a A + T content of 59.6 % and presents direct and inverted internal repeats. Restriction analysis of the total DNA with methylation sensitive enzymes suggests that this repetitive DNA is undermethylated. In situ hybridization with a biotinylated probe of the satellite DNA showed the pericentromeric localization of these sequences in all meiotic bivalents. The presence of this repetitive DNA in other species of the genus was also tested by Southern analysis. The results showed that this satellite DNA sequence is specific to the C. americana genome and has not been found in three other species of Chrysolina with a different choice of host plants than in the former.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altschul, S.F., W. Gish, W. Miller, M.W. Myers & D.J. Lipman, 1990. A basic local alignment search tool. J. Mol. Biol. 215: 403–410.

    Article  PubMed  CAS  Google Scholar 

  • Altschul, S.F., F. Stephen, L. Thomas, L. Madden, A.A. Schaffer, J. Zhang et al., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.

    Article  PubMed  CAS  Google Scholar 

  • Bachmann, L., J.M. Schibel, M. Raab & D. Sperlich, 1993. Satellite DNA as a taxonomic marker. Biochem. Syst. Ecol. 21: 3–11.

    Article  CAS  Google Scholar 

  • Barceló, F., J. Pons, E. Petitpierre, I. Barjau & J. Portugal, 1997. Polymorphic curvature of satellite DNA in three subspecies of the beetle Pimelia sparsa. Eur. J. Biochem. 244: 318–324.

    Article  PubMed  Google Scholar 

  • Bigot, Y., M. Hamelin & G. Periquet, 1990. Heterochromatin condensation and evolution of unique satellite-DNA families in two parasitic wasp species: Diadromus pulchellus and Eupelmus vuilleti. (Hymenoptera). Mol. Biol. Evol. 7: 351–364.

    PubMed  CAS  Google Scholar 

  • Brutlag, D.L., 1980. Molecular arrangement and evolution of heterochromatic DNA. Annu. Rev. Genet. 14: 121–144.

    Article  PubMed  CAS  Google Scholar 

  • Daccordi, M., 1996. Notes on the distribution of the Crysomelinae and their possible origin, pp. 399–412 in Chrysomelidae Biology, Vol. 1, edited by P.H.A. Jolivet & M.L. Cox. SPB Academic, Amsterdam.

    Google Scholar 

  • Dover, G.A., 1986. Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet. 168: 159–165.

    Article  Google Scholar 

  • Ehrlich, P.R. & P.H. Raven, 1964. Butterflies and plants: a study in coevolution. Evolution 18: 586–608.

    Article  Google Scholar 

  • Farrell, B.D., 1998. 'Inordinate Fondness’ explained: why are there so many beetles? Science 281: 555–559.

    Article  PubMed  CAS  Google Scholar 

  • Garin, C.F., C. Juan & E. Petitpierre, 1999. Mitochondrial DNA phylogeny and the evolution of host-plant use in palearctic Chrysolina (Coleoptera, Chrysomelidae). J. Mol. Evol. 48: 435–444.

    Article  PubMed  CAS  Google Scholar 

  • Gómez-Zurita, J., C.F. Garin, C. Juan & E. Petitpierre, 1999. Mitochondrial 16S rDNA sequences and their use as phylogenetic markers in leaf-beetles with special reference to the subfamily Chrysomelidae, pp. 25–38 in Advances in Chrysomelidae Biology, edited by M.L. Cox. Backhuys Publ. Oegstgeest, The Netherlands.

    Google Scholar 

  • Goméz-Zurita, J., C. Juan & E. Petitpierre, 2000. The evolutionary history of the genus Timarcha (Coleoptera, Chrysomelidae) inferred from mitochondrial COII gene and partial 16S rDNA sequences. Mol. Phylogenet. Evol. 4: 433–447.

    Google Scholar 

  • Heinze, J., J. Gadau, B. Hölldobler, I. Nanda, M. Schmid & K. Scheller, 1994. Genetic variability in the ant Camponotus floridanus detected by multilocus fingerprinting. Naturwissenschaften 81: 34–36.

    CAS  Google Scholar 

  • Jolivet, P., 1988. Food habits and food selection of Chrysomelidae. Bionomic and evolutionary perspectives, pp. 1–24 in Biology of Chrysomelidae, edited by P. Jolivet, E. Petitpierre & T.S. Hsiao. Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Jolivet, P. & E. Petitpierre, 1976. Les plantes-hôtes connus des Chrysolina (Col Chrys) Essai sur les types de sélection trophique. Ann. Soc. Entomol. Fr. (NS) 12: 123–149.

    Google Scholar 

  • Juan, C., J. Pons & E. Petitpierre, 1993. Location of tandemly repeated DNA sequences in beetle chromosomes by fluorescent in situ hybridization. Chromosome Res. 1: 167–174.

    Article  PubMed  CAS  Google Scholar 

  • King, L.M. & M.P. Cumming, 1997. Satellite DNA repeat sequence variation is low in three species of burying beetles in the genus Nicrophorus (Coleoptera: Silphidae). Mol. Biol. Evol. 14: 1088–1095.

    Google Scholar 

  • Landais, I., P. Chavigny, C. Catagnone, J.P. Pizzol, P. Abad & F. Vanlerrberghe-Massutti, 2000. Characterization of highly conserved satellite DNA from the parasitoid wasp Trichogramma brasicae. Gene 255: 65–73.

    Article  PubMed  CAS  Google Scholar 

  • Lorite, P., M.F. García & T. Palomeque, 1999. Satellite DNA in the ant Messor structor (Hymenoptera, Formicidae). Genome 42: 881–886.

    Article  PubMed  CAS  Google Scholar 

  • Mestrovic, N., M. Plohl, B. Mravinac & D. Ugarkovic, 1998. Evolution of satellite DNAs from the genus Palorus. Experimental evidence for the ‘library’ hypothesis. Mol. Biol. Evol. 15: 1062–1068.

    CAS  Google Scholar 

  • Pearson, W.R. & D.J. Lipman, 1998. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85: 2444–2448.

    Article  Google Scholar 

  • Petitpierre, E., 1975. Notes on chromosomes of ten species of the genus Chrysolina Mots. (Coleoptera, Chrysomelidae). Genetica 45: 349–354.

    Article  Google Scholar 

  • Petitpierre, E., 1981. New data on the cytology of Chrysolina Mots. and Oreina Chevr. (Coleoptera, Chrysomelidae). Genetica 54: 265–272.

    Article  Google Scholar 

  • Petitpierre, E., 1983. Karyometric differences among nine species of the genus Chrysolina Mots. (Coleoptera, Chrysomelidae). Can. J. Genet. Cytol. 25: 33–39.

    Google Scholar 

  • Petitpierre, E., 1995. Molecular cytogenetics and taxonomy of insects, with particular reference to the Coleoptera. Int. J. Insect Morphol. Embryol. 25: 115–134.

    Article  Google Scholar 

  • Petitpierre, E., 1999. The cytogenetic and cytotaxonomy of Chrysolina Mots. and Oreina Chevr. (Coleoptera, Chrysomelidae, Chrysomelinae). Hereditas 131: 55–62.

    Article  Google Scholar 

  • Petitpierre, E., C. Segarra & C. Juan, 1993. Genome size and chromosomal evolution in leaf beetles (Coleoptera, Chrysomelidae). Hereditas 119: 1–6.

    Article  Google Scholar 

  • Pinkel, D., T. Straume & J.M. Gray, 1986. Cytogenetic analysis using quantitative, high sensitivity fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83: 2934–2938.

    Article  PubMed  CAS  Google Scholar 

  • Plohl, M. & D. Ugarkovic, 1994. Characterization of two abundant DNAs from the mealworm Tenebrio obscurus. J. Mol. Evol. 39: 489–495.

    Article  PubMed  CAS  Google Scholar 

  • Plohl, M., N. Mestrovic, B. Bruvo & D. Ugarkovic, 1998. Similarity of structural features and evolution of satellite DNAs from Palorus subdepressus (Coleoptera) and related species. J. Mol. Evol. 46: 234–239.

    Article  PubMed  CAS  Google Scholar 

  • Pons, J., B. Bruvo, C. Juan, E. Petitpierre, M. Plohl & D. Ugarkovic, 1997. Conservation of satellite DNA in species of the genus Pimelia (Tenebrionidae, Coleoptera). Gene 205: 183–190.

    Article  PubMed  CAS  Google Scholar 

  • Pons, J., E. Petitpierre & C. Juan, 1999. Gradual or saltatory evolution of satellite DNA in the genus Pimelia? Proceedings of the VII ESEB Congress, Barcelona, Spain, August 23–28, 1999. pp. 11–237.

  • Rojas–Rousse, D., Y. Bigot & G. Periquet, 1993. DNA insertions as a component of the evolution of the unique satellite DNA families in two genera of parasitoid wasps: Diadromus and Eupelmus (Hymenoptera). Mol. Biol. Evol. 10: 383–396.

    PubMed  CAS  Google Scholar 

  • Salser, W., S. Bowen, D. Browne, F. el-Adli, N. Fedoroff, K. Fry et al., 1976. Investigation of the organization of mammalian chromosomes at the DNA sequence level. Fed. Proc. 35: 23–35.

    CAS  Google Scholar 

  • Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular cloning, 2nd edn, Cold Spring Harbor Laboratory Press.

  • Singer, M.F., 1982. Highly repeated sequences in mammalian genomes. Int. Rev. Cytol. 76: 67–112.

    Article  PubMed  CAS  Google Scholar 

  • Smith, S.G. & N. Virkki, 1978. Coleoptera, in Animal Cytogenetics, vol. 3 Insecta 5, edited by B. John. & G. Borntraeger, Berlin.

  • Sonoda, S., T. Yamada, T. Naito & F. Nakasuji, 1995. Characterization of a family of tandemly repetitive DNA sequences from the fern sawfly, Strongylogaster osmundae (Hymenoptera: Tenthredinidae). Jpn. J. Genet. 70: 167–177.

    Article  PubMed  CAS  Google Scholar 

  • Strong, D.R., J.L. Lawton & T.R.E. Southwood, 1984. Insects on plants: Community patterns and mechanisms. Harvard University Press, Cambridge.

    Google Scholar 

  • Sumner, A.T., 1990. Banding with fluorochromes other than quinacrine, pp. 163–165, in Chromosome banding, edited by U. Hyman. Unwin Hyman, London.

    Google Scholar 

  • Ugarkovic, D., M. Plohl, V. Gamulin & C.W. Achwal, 1989. Sequence variability of satellite DNA from the mealworm Tenebrio molitor. Gene 83: 181–183.

    Article  PubMed  CAS  Google Scholar 

  • Ugarkovic, D., E. Petitpierre, C. Juan & M. Plohl, 1995. Satellite DNAs in Tenebrionid species: structure, organization and evolution. Croatica Chemica Acta 68: 627–638.

    CAS  Google Scholar 

  • Ugarkovic, D., M. Podnar & M. Plohl, 1996. Satellite DNA of the red flour beetle Tribolium castaneum. Comparative study of satellites from the genus Tribolium. Mol. Biol. Evol. 13: 1059–1066.

    CAS  Google Scholar 

  • Virkki, N., 1984. Chromosomes in evolution of Coleoptera., pp. 41–76 in Chromosomes in evolution of eukaryotic groups, vol. 2, edited by A.K. Sharma & A. Sharma. C.R.C. Press, Boca Raton, Florida.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universidad de Jaén, ES-23071, Jaen, Spain

    Pedro Lorite

  2. Área de Genética, Departmento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, ES-23071, Jaen, Spain

    Teresa Palomeque

  3. Laboratori de Genètica, Department de Biología, Facultat de Ciències, IMEDEA-Universitat de les Illes Balears, ES-07071, Palma de Mallorca, Spain

    Irene Garner & Eduard Petitpierre

Authors
  1. Pedro Lorite
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Teresa Palomeque
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Irene Garner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eduard Petitpierre
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lorite, P., Palomeque, T., Garner, I. et al. Characterization and chromosome location of satellite DNA in the leaf beetle Chrysolina americana (Coleoptera, Chrysomelidae). Genetica 110, 143–150 (2000). https://doi.org/10.1023/A:1017926127213

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017926127213

Search

Navigation