Abstract
DNA contents, ranging from 4C to more than 500C, were determined by a quantitative microfluorimetric assay in supramedullary neuron nuclei of the pufferfish Diodon holacanthus. The distribution of C values after ethidium bromide staining indicates an inter- and intra-individual variation in DNA contents which do not correspond to duplications of the total genome, suggesting that DNA replication is specific for particular genes (endoreplication). Moreover, the DNA content appears to be correlated with nuclear size. A relationship between the DNA amounts and the presence of AT- and GC-rich sequences has been shown.
This work demonstrates, for the first time, DNA endoreplication in a specific neuronal type in vertebrates.
Similar content being viewed by others
References
Benedetti I, Mola L (1988) Survey of neuropeptide-like immunoreactivity in supramedullary neurons of Coris julis (L.). Brain Res 449: 373–376.
Bennett MVL, Crain SM, Grundfest H (1959a) Electrophysiology of supramedullary neurons in Spheroides maculatus. I. Orthodromic and antidromic responses. J Gen Physiol 43: 159–188.
Bennett MVL, Crain SM, Grundfest H (1959b) Electrophysiology of supramedullary neurons in Spheroides maculatus. II. Properties of the electrically excitable membrane. J Gen Physiol 43: 189–219.
Bennett MVL, Crain SM, Grundfest H (1959c) Electrophysiology of supramedullary neurons in Spheroides maculatus. III. Organization of the supramedullary neurons. J Gen Physiol 43: 221–250.
Bennett MVL, Nakajima Y, Pappas GD (1967) Physiology and ultrastructure of electrotonic junctions. I. Supramedullary neurons. J Neurophysiol 30: 161–179.
Bernocchi G, Barni S (1985) On the heterogeneity of Purkinje neurons in Vertebrates. J Hirnforsch 26: 227–235.
Bernocchi G, De Stefano GF, Porcelli F, Redi CA, Manfredi-Romanini MG (1976) Feulgen reaction hydrolysis kinetics in the interphase and metaphase. The Nucleus 19: 141–149.
Brodsky VY, Uryvaeva IV (1985) Genome multiplication in growth and development, Cambridge: Cambrige University Press.
Chase R, Tolloczko B (1987) Evidence for differential DNA endoreplication during the development of a molluscan brain. J Neurobiol 18: 395–406.
Carrol RL (1997) Vertebrate paleontology and evolution, New York: Freeman W. H. & company.
Funakoshi K, Kadota T, Atobe Y, Nakano M, Goris RC, Kishida R (1998) Gastrin/CCK-ergic innervation of cutaneous mucous gland by the supramedullary cells of the puffer fish Takifugu niphobles. Neurosci Lett 258: 171–174.
Funakoshi K, Toshio A, Reiji K (1995) Trigeminal, vagal and spinal projections of supramedullary cells in the puffer fish Takifugu niphobles. J Comp Neurol 358: 552–562.
Gillette R (1991) On the significance of neuronal giantism in Gastropods. Biol Bull 180: 234–240.
Greenwood PH, Rosen DE, Weitzman SH, Myers GS (1966) Phyletic studies of teleostean fishes, with a provisional classification of living forms. Bull Am Mus Nat Hist 131: 339–456.
Herman CJ, Lapham LW (1969) Neuronal polyploidy and nuclear volumes in the cat central nervous system. Brain Res 15: 35–48.
Kunz W(1984) How genes are amplified in germ cells and somatic cells. In: Engels W, ed., New York: Elsevier Press.
Lentz RD, Lapham LW (1969) A quantitative cytochemical study of the DNA content of neurons of rat cerebellar cortex. J Neurochem 16: 379–84.
Manicardi GC, Bizzaro D, Sonetti D, Lombardo F, Bianchi U (1992) Amplification of GC-rich DNAs in neuronal nuclei of Planorbarius corneus (L.) (Mollusca, Pulmonata). Eur J Histochem 36: 303–309.
Nelson J (1984) Fishes of the world, 2nd edn., New York: JohnWiley & Sons Ltd.
Osheim YN, Miller OL (1983) Novel amplification and transcriptional activity of chorion genes in Drosophila melanogaster follicle cells. Cell 33: 543–553.
Sandritter W, Nováková V, Pilný J, Kiefer G (1967) Cytophotometrische Messungen des Nuklein-Saure und Proteingehaltes von Ganglienzellen der Ratte Wahrend der postnatalen Entwicklung und im Alter. Z Zellforsch 80: 145–152.
Sassi D, Manicardi GC, Mola L, Benedetti I (1995) Cytofluorimetric evidence for differential genome endoreplication in the cluster neurons of Lophius piscatorius L. (Osteichthyes, Lophiiformes). Eur J Histochem 39: 117–126.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mola, L., Cuoghi, B., Mandrioli, M. et al. DNA Endoreplication in the Clustered Supramedullary Neurons of the Pufferfish Diodon Holacanthus L. (Osteichthyes). Histochem J 33, 59–63 (2001). https://doi.org/10.1023/A:1017595914731
Issue Date:
DOI: https://doi.org/10.1023/A:1017595914731