Abstract
The aim of this study was to examine the embryogenic potential of floral material of the cassava cultivar MCOL 1505. Macerated immature inflorescences were found to be highly embryogenic, with almost 78% of the explants producing somatic embryos. Somatic embryos were also produced from whole male florets and half florets although at much lower rates. No regeneration was obtained from anther, microspore or floret wall tissue. Somatic embryos derived from immature inflorescences were regenerated via organogenesis and the plants derived from this process were assessed in terms of phenotype and ploidy level. If haploid plants could be produced by this method, this would have significant implications in assisting traditional cassava breeding, as this would allow homozygosity to be reached more rapidly. In a crop such as cassava, which is highly heterozygous in nature, the use of haploids in a breeding programme could considerably shorten the time taken to produce new desirable cultivars. This is the first report on plant regeneration through somatic embryogenesis from floral tissue of cassava.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson, W.C. & J.B. Carstens, 1977. Tissue culture propagation of broccoli, Brassica oleracea(Italica group), for use in F1 hybrid seed production. J Am Soc Hort Sci 102: 69–73.
Canas, B.M. & W.M. Roca, 1982. Anther culture of cassava. Revista de Investigacion (Colombia) 12: 5–16.
Coumans-Gilles, M.F., C. Kevers, M. Coumans, E. Ceulemans & Th. Gaspar, 1981. Vegetative multiplication of sugar beet through in vitro culture of inflorescence pieces. Plant Cell Tiss Organ Cult 1: 93–101.
Drira, N. & A. Benbadis,1985. Multiplication végétative du palmier dattier (Phoenix dactylifera L) par revision, en culture in vitro, d, ebauch es florales de pieds femelles. J Plant Physiol 119: 227–235.
George, E.F., 1993. Plant Propagation by TissueCulture. Part 1. Publ. Exergetics Ltd. England. pp. 52.
Li, H.-Q., J.-Y. Guo, Y.-W. Huang, C.-Y. Liang, H.-X. Liu, I. Potrykus & J. Puonti-Kaerlas, 1998. Regeneration of cassava plants via shoot organogenesis. Plant Cell Rep 17: 410–414.
Liu, M.C. & W.H. Chen, 1977. Genetic modification of sugarcane and cassava cellsthrough protoplast and anther culture. Taiwan sugar 24: 304–311.
Lynam, J.H., 1993. Potential Impact ofBiotechnology on Cassava Production in the Third World. In: W.M. Roca & A.M. Thro (Eds.), Proc First Intl Sci Meet Cassava Biotechn Netw, Cartegena, Colombia, 25–28 August, 1992. Cali, Colombia: Centro International de Agricultura Tropical, 1993. Working Document no. 123.
Mathews, H., C. Schöpke, R. Carcamo, P. Chavarriaga, C. Fauquet & R.N. Beachy, 1993. Improvement of somatic embryogenesis and plant recovery in cassava. Plant Cell Rep 12: 328–333.
Moh, C.C., 1975. Induction of anther callus in cassava. Trop Root Tuber Crops Newsl 8: 5–7.
Mukherjee, A., M. Unnikrishnam, N.G. Nair & C.S. Easwariamma, 1989. Influence of genotypes and media in pollen embryogenesis in cassava. J Root Crops 15: 133–138.
Mukherjee, A., 1995. Embryogenesis andRegeneration from Cassava Calli of Anther and Leaf. In: Proc Second Intl Sci Meet Cassava Biotech Netw, Bogor, Indonesia, 22–26 August, 1994, pp. 375–377. Cali, Colombia: Centro Internacional de Agricultura Tropical, 1995. Working Document no. 150.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays withtobacco tissue culture. Physiol Plant 15: 473–497.
Raemakers, C.J.J.M., M. Amati, G. Staritsky, E. Jacobsen & R.G.F. Visser, 1993. Cyclic somatic embryogenesis and plant regeneration in cassava. Ann of Botany 71: 289–294.
Roca, W.M., G. Henry, F. Angel & R. Sarria, 1992. Biotechnology research applied to cassavaimprovement at the International Center of Tropical Agriculture (CIAT). AgBiotech News Inf 4: 303–308.
Stamp, J.A. & G.G. Henshaw, 1982. Somatic embryogenesis in cassava. Z Pflanzenphys 105: 183–187.
Stamp, J.A. & G.G. Henshaw, 1987. Secondary somatic embryogenesis and plant regeneration in cassava. Plant Cell Tiss Org Cult 10: 227–233.
Szabados, L., R. Hoyos & W.M. Roca, 1987. In vitro somaticembryogenesis and plant regeneration of cassava. Plant Cell Rep 6: 248–251.
Taylor, N.J. & G.G. Henshaw,1993. The Induction of Somatic Embryogenesis in Fifteen African and one South American Cassava Cultivars. In: W.R. Roca & A.M. Thro (Eds.), Proc First Intl Sci Meet Cassava Biotech Netw, Cartegena, Colombia, 25–28 August, 1992. Cali, Colombia: Centro Internacional de Agricultura Tropical, 1993. Working document no. 123.
Taylor, N.J.,M. Edwards, R.J. Kiernan, C.D.M. Davey, D. Blakesly & G.G. Henshaw, 1996. Development of friable embryogenic callus and embryogenic suspension culture systems in cassava (Manihot esculenta Crantz). Nature Biotechn 14: 726–730.
Thro, A.M., M. Fregene, N. Taylor, C.J.J.M. Raemakers, J. Puonti-Kaerlas, C. Schöpke, R. Visser, I. Potrykus, C. Fauquet, W.M. Roca & C. Hershey, 1999. Genetic biotechnologies and cassava - based development. In: T. Hohn & K.M. Leisinger (Eds.), Biotechnology of Food Crops in Developing Countries, pp. 139–185. Springer Verlag, Wien.
Woodward, B. & S. Thiart, 1998. Micropropagation of a Rare South African Haworthia. Posterpresentation at the IX International Congress on Plant Tissue and Cell Culture, Jerusalem, Israel.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Woodward, B., Puonti-Kaerlas, J. Somatic embryogenesis from floral tissue of cassava (Manihot esculenta Crantz). Euphytica 120, 1–6 (2001). https://doi.org/10.1023/A:1017582412971
Issue Date:
DOI: https://doi.org/10.1023/A:1017582412971