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Changes in peroxidase activity, and level of phenolic compounds during light-induced plantlet regeneration from Eucalyptus camaldulensis Dehn. nodes in vitro

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Abstract

Node cultures of Eucalyptus camaldulensis Dehn inPetri dishes in vitro under darkness in the presence of anauxin developed meristematic agglomerates (4 to 6 diameter),i.e. dense shoot clusters in which outgrowth of numerous successive buds islimited. Similar cultures under a 16 photoperiod produced smallgreen plantlets with reduced leaves often presenting white hypertrophiedlenticels and very short roots crowning the stem bases. The use of half-litreglass vials under light allowed direct development of well-developed rootedplantlets, either in the presence of the same auxin or in the presence of acytokinin. Light favoured an increase in phenolic compounds and a reversevariation of peroxidase activity during the culture cycles. These aspects arediscussed in terms of a possible regulation of the endogenous auxin levelthrough a light control of peroxidase activity and the level of phenoliccompounds.

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Arezki, O., Boxus, P., Kevers, C. et al. Changes in peroxidase activity, and level of phenolic compounds during light-induced plantlet regeneration from Eucalyptus camaldulensis Dehn. nodes in vitro. Plant Growth Regulation 33, 215–219 (2001). https://doi.org/10.1023/A:1017579623170

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