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Anoxia tolerance and alcohol dehydrogenase activity in lettuce seedlings

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Abstract

In order to clarify the effect of protein synthesis inhibition on anoxiatolerance, lettuce (Lactuca sativa L.) seedlings weresubjected to anoxic stress in the presence of cycloheximide (CHM). Atconcentrations greater than 3 , CHM decreased thesurvivability of the roots and the survivability decreased with increasing CHMdoses. At 100 CHM, the survivability was 41% of thatof non-CHM-treated seedlings. Alcohol dehydrogenase (EC 1.1.1.1) activity andATP concentration in the roots of the seedlings were also decreased by CHM,which may be one of the causes of the reduced anoxia tolerance of the seedlings.

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References

  • Andrews C.J. and Pomeroy M.K. 1989. Metabolic acclimation to hypoxia in winter cereals. Low temperature flooding increases adenylates and survival in ice encasement. Plant Physiol. 91: 1063–1068.

    Google Scholar 

  • Averina N.G., Yaronskaya E.B., Rassadina V.V. and Walter G. 1996. Response of magnesium chelatase activity in green pea (Pisum sativum L.) leaves to light, 5-aminolevulinic acid and dipyridyl supply. J. Photochem. Photobiol. B. Biol. 36: 17–22.

    Google Scholar 

  • Bergmeyer H.U. 1985. Methods of Enzymatic Analysis. VII. Metabolites. VCH Publishers, Weinheim.

    Google Scholar 

  • Bouma T.J., de Visse R., Janssen J.H.J.A., de Kock M.J., van Leeuwen P.H. and Lambers H. 1994. Respiratory energy requirements and rate of protein turnover in vivo determined by the use of an inhibitor of protein synthesis and a probe to assess its effect. Physiol. Plant. 92: 585–594.

    Google Scholar 

  • Bouny J.M. and Saglio P.H. 1996. Glycolytic flux and hexokinase activities in anoxic maize root tips acclimated by hypoxic pretreatment. Plant Physiol. 111: 187–194.

    Google Scholar 

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Google Scholar 

  • Crawford R.M.M. 1982. Physiological responses to flooding. In: Lange O.L., Nobel P.S., Osmond C.B. and Ziegler H. (eds), Physiological Plant Ecology II. Water Relations and Carbon Assimilation. Encyclopedia Plant Physiology, Vol. 12B. Springer-Verlag, New York, pp. 453–477.

    Google Scholar 

  • Davies D.D. 1980. Anaerobic metabolism and the production of organic acids. In: Davies D.D. (ed.), The Biochemistry of Plants Vol. 2. Academic Press, New York, pp. 581–611.

    Google Scholar 

  • Drew M.C., Saglio P.H. and Pradet A. 1985. Higher adenylate energy change and ATP/ADP rations in aerenchymatous roots of Zea mays in anaerobic media as a consequence of improved internal oxygen transport. Planta. 165: 51–58.

    Google Scholar 

  • Drew M.C. 1997. Oxygen deficiency and root metabolism: Injury and acclimation under hypoxia and anoxia. Annu. Rev. Plant Physiol Plant Mol. Biol. 48: 223–250.

    Google Scholar 

  • Ellis M.H., Dennis E.S. and Peacock W.J. 1999. Arabidopsis roots and shoots have different mechanisms for hypoxic stress tolerance. Plant Phyisol. 119: 57–64.

    Google Scholar 

  • Felle H.H. 1996. Control of cytoplasmic pH under anoxic conditions and its implication for plasma membrane proton transport in Medicago sativa root hairs. J. Exp. Bot. 47: 967–973.

    Google Scholar 

  • Fox G.C., McCallan N.R. and Ratcliffe R.G. 1995. Manipulating cytoplasmic pH under anoxia. A critical test of the role of pH in the switch from aerobic to anaerobic metabolism. Planta. 195: 324–330.

    Google Scholar 

  • Germain V., Ricard B., Raymond P. and Saglio P.H. 1997. The role of sugars, hexokinase, and sucrose synthase in the determination of hypoxically induced tolerance to anoxia in tomato roots. Plant Physiol. 114: 167–175.

    Google Scholar 

  • Good A.G. and Crosby W.L. 1989. Anaerobic induction of alanine aminotransferase in barley root tissue. Plant Physiol. 90: 1305–1309.

    Google Scholar 

  • Good A.G. and Muench D.G. 1993. Long-term anaerobic metabolism in root tissue. Metabolic products of pyruvate metabolism. Plant Physiol. 101: 1163–1168.

    Google Scholar 

  • Hanson A.D., Jacobsen J.V. and Zwar J.A. 1984. Regulated expression of three alcohol dehydrogenase genes in barley aleurone layers. Plant Physiol. 75: 573–581.

    Google Scholar 

  • Hoeren F.U., Dolferus R., Wu Y., Peacock W.J. and Dennis E.S. 1998. Evidence for a role for AtMYB2 in the induction of the Arabidopsis alcohol dehydrogenase gene (ADH1) by low oxygen. Genetics. 149: 470–490.

    Google Scholar 

  • Jackson M.B. 1990. Hormones and developmental change in plants subjected to submergence or soil waterlogging. Aquat. Bot. 38: 49–72.

    Google Scholar 

  • Johnson J.R., Cobb B.G. and Drew M.C. 1989. Hypoxic induction of anoxia tolerance in root tips of Zea mays. Plant Physiol. 91: 837–841.

    Google Scholar 

  • Johnson J.R., Cobb B.G. and Drew M.C. 1994. Hypoxic induction of anoxia tolerance in roots of Adh1 null Zea mays L. Plant Physiol. 105: 61–67.

    Google Scholar 

  • Kato-Noguchi H. and Watada A.E. 1997. Effects of low-oxygen atmosphere on ethanolic fermentation in fresh-cut carrots. J. Amer. Soc. Hort. Sci. 122: 107–111.

    Google Scholar 

  • Kennedy R.A., Rumpho M.E. and Fox T.C. 1992. Anaerobic metabolism in plants. Plant Physiol. 100: 1–6.

    Google Scholar 

  • Mohanty B., Wilson P. and Ap Rees T. 1993. Effects of anoxia on growth and carbohydrate metabolism in suspension cultures of soybean and rice. Phytochemistry. 34: 75–82.

    Google Scholar 

  • Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plant. 15: 473–497.

    Google Scholar 

  • Perata P. and Alpi A. 1991. Ethanol-induced injuries to carrot cells. The role of acetaldehyde. Plant Physiol. 95: 748–752.

    Google Scholar 

  • Ricard B., Couée I., Raymond P., Saglio P.H., Saint-Ges V. and Pradet A. 1994. Plant metabolism under hypoxia and anoxia. Plant Physiol. Biochem. 32: 1–10.

    Google Scholar 

  • Rivoal J. and Hanson A.D. 1994. Metabolic control of anaerobic glycolysis. Overexpression of lactate dehydrogenase in transgenic tomato roots supports the Davies-Roberts hypothesis and points to a critical role for lactate secretion. Plant Physiol. 106: 1179–1185.

    Google Scholar 

  • Roberts J.K.M., Callis J., Wemmer D., Walbot V. and Jardetzky O. 1984. Mechanism of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia. Proc. Natl. Acad. Sci. USA. 81: 3379–3383.

    Google Scholar 

  • Sachs M.M. and Ho T.-H.D. 1986. Alteration of gene expression during environmental stress in plants. Annu. Rev. Plant Physiol. 37: 363–376.

    Google Scholar 

  • Sachs M.M., Subbaiah C.C. and Saab I.N. 1996. Anaerobic gene expression and flooding tolerance in maize. J. Exp. Bot. 47: 1–15.

    Google Scholar 

  • Sobczyk E.A., Marszalek A. and Kacperska A. 1985. ATP involvement in plant tissue response to low temperature. Physiol. Plant. 63: 399–405.

    Google Scholar 

  • Spanswick R.M. 1981. Electrogenic ion pumps. Annu. Rev. Plant Physiol. 32: 267–289.

    Google Scholar 

  • Tadege M., Brändle R. and Kuhlemeier C. 1998. Anoxia tolerance in tobacco roots: Effect of overexpression of pyruvate decarboxylase. Plant J. 14: 327–335.

    Google Scholar 

  • Vartapetian B.B. and Jackson M.B. 1997. Plant adaptations to anaerobic stress. Ann. Bot. 79: 2–20.

    Google Scholar 

  • Waters I., Morrell S., Greenway H. and Colmer T.D. 1991. Effects of anoxia on wheat seedlings. II. Influence of O2 supply prior to anoxia on tolerance to anoxia, alcoholic fermentation, and sugar levels. J. Exp. Bot. 42: 1437–1447.

    Google Scholar 

  • Xie Y. and Wu R. 1989. Rice alcohol dehydrogenase genes. Anaerobic induction, organ specific expression and characterization of cDNA clones. Plant Mol. Biol. 13: 53–68.

    Google Scholar 

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Kato-Noguchi, H. Anoxia tolerance and alcohol dehydrogenase activity in lettuce seedlings. Plant Growth Regulation 33, 199–203 (2001). https://doi.org/10.1023/A:1017558523090

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