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An efficient method for assigning traits to chromosomes

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Abstract

Chromosome locations of two allele-specific SCAR (sequencecharacterized amplified region) DNA markers in wheat (Triticumaestivum L.) were found by identifying critical aneuploids obtained by thepollination of haploids. One SCAR, linked to a gene for antibioticresistance to wheat midge (Sitodiplosis mosellana Géhin) was onchromosome 2B. The second SCAR, linked to one of three genes for whiteseed color was on 3B. Unemasculated haploids of M11 (resistant & whiteseeded) were pollinated with BW252 (susceptible & a three-genered) yielding about 1 hybrid seed per 4 heads pollinated. Out of 94 seedstested, endosperm DNA of 15 seeds lacked one SCAR or the other,signaling a critical deficiency. These 15 selected seeds gave 9 viable hybridsof which 2 were false negatives. By screening for polymorphicmicrosatellites, informative markers were obtained for all chromosomesexcept 7D. Chromosomes missing from marker negative hybrids wereinferred from monomorphism of chromosome-specific microsatellites plusmeiotic data. Hybrids lacking the midge SCAR lacked 2B from M11 whilehybrids lacking the white SCAR lacked all or part of 3B from M11. Whilesome of the aneuploids were complex, simple monosomics were recoveredfor both 2B and 3B.

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Authors and Affiliations

  1. Cereal Research Center, Agriculture and Agrifood Canada, Winnipeg, Manitoba, R3T 2M9, Canada

    J. Thomas, E. Riedel & G. Penner

Authors
  1. J. Thomas
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  2. E. Riedel
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  3. G. Penner
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Thomas, J., Riedel, E. & Penner, G. An efficient method for assigning traits to chromosomes. Euphytica 119, 217–221 (2001). https://doi.org/10.1023/A:1017552713206

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