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Programmed cell death in Datura innoxia Mill. callus cultures cultivated in light and in dark

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Abstract

It has been established that dimedone in solid culture medium influencedthe growth of Datura innoxia Mill. callus tissues and theapoptotic processes of cells. This formaldehyde (HCHO) capture reagent appearsto modify the metabolism of plant cells, resulting in quantitative changes inthe apoptotic index (Ai). Apoptotic cells were detected insix-week-old callus tissues by the TUNEL reaction. The amount of TUNEL positivenuclei showed a characteristic spatial distribution. Enhanced DNA fragmentationwas observed in the cell layers close to the surface of the cultures. ElevatedAi was determined in cultures grown in the dark compared to thetissues grown in the light. High doses of dimedone considerably decreasedapoptosis in tissue cultures under both light and dark conditions.

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Authors and Affiliations

  1. Institute of Pharmacognosy, Semmelweis University of Medicine, H-1085, Budapest, Hungary

    I. László & É Szőke

  2. Plant Protection Institute, Hungarian Academy of Sciences, H-1525, Budapest, Hungary

    E. Tyihák

  3. Hungarian Academy of Sciences, Semmelweis University of Medicine, 1st Institute of Pathology and Experimental Cancer Research, Molecular Pathology Research Group of the Joint Organization, H-1085, Budapest, Hungary

    B. Szende

Authors
  1. I. László
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  2. É Szőke
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  3. E. Tyihák
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  4. B. Szende
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László, I., Szőke, É., Tyihák, E. et al. Programmed cell death in Datura innoxia Mill. callus cultures cultivated in light and in dark. Plant Growth Regulation 33, 231–236 (2001). https://doi.org/10.1023/A:1017552513002

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