Skip to main content
SpringerLink
Log in

Immunocytochemical Localization of Epidermal Growth Factor Receptor in Early Embryos of the Japanese Medaka Fish (Oryzias Latipes)

  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Abstract

This study was undertaken to localize epidermal growth factor receptor (EGFR) during early development of Japanese medaka embryos using immunocytochemistry. Specific staining was observed in all stages studied. All of the cells of the embryonic disc from the germinal disc (1 cell) through the late high blastula stages stained moderately for EGFR. Beginning with the flat blastula stage, the surface and lateral cells of the embryonic disc and the cells migrating around the yolk stained intensely for EGFR, and this continued throughout the study period. The presence of the keel at the late gastrula stage did not affect the moderate staining of the majority of the embryonic disc cells. When somites first appeared, the keel region stained less intensely than before, but scattered individual cells stained intensely for EGFR. Embryos with 12 somites had a neural tube that was lightly stained except for a few intensely stained individual cells. The neural tube, notochord and somites in 24-somite embryos lacked immunostaining. However, the surface epithelium, aorta, intestinal epithelium and pronephric duct demonstrated EGFR immunostaining. This study demonstrates that EGFR is present during medaka development and supports the hypothesis that EGFR ligands are important during cleavage, gastrulation and early organogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adamson ED, Wiley LM (1997) The EGFR gene family in embryonic cell activities. Curr Top Dev Biol 35: 71–120.

    Google Scholar 

  • Ahmad I, Dooley CM, Afiat S (1998) Involvement of Mash1 in EGFmediated regulation of differentiation in the vertebrate retina. Dev Biol 194: 86–98.

    Google Scholar 

  • Barrandon Y, Green H (1987) Cell migration is essential for sustained growth of keratinocyte colonies: the roles of transforming growth factor-α and epidermal growth factor. Cell 50: 1131–7.

    Google Scholar 

  • Boomsma RA, Mavrogianis PA, Verhage HG (1997) Immunocytochemical localization of transforming growth factor-α, epidermal growth factor, and epidermal growth factor receptor in the cat endometrium and placenta. Histochem J 29: 495–504.

    Google Scholar 

  • Brice EC, Wu J-X, Muraro R, Adamson ED, Wiley LM (1993) Modulation of mouse preimplantation development by epidermal growth factor receptor antibodies, antisenseRNA, and deoxyoligonucleotides. Dev Gen 14: 174–84.

    Google Scholar 

  • Chia CM, Winston RM, Handyside AH (1995) EGF, TGF-α and EGFR expression in human preimplantation embryos. Development 121: 299–307.

    Google Scholar 

  • Coffey RJ Jr, Derynck R, Wilcox JN, Bringman TS, Goustin AS, Moses HL, Pittelkow MR (1987) Production and auto-induction of transforming growth factor-α in human keratinocytes. Nature 328: 817–20.

    Google Scholar 

  • Coffey RJ Jr, Sipes NJ, Bascom CC, Graves-Deal R, Pennington CY, Weissman BE, Moses HL (1988) Growth modulation of mouse keratinocytes by transforming growth factors. Cancer Res 48: 1596–602.

    Google Scholar 

  • Dardik A, Smith RM, Schultz RM (1992) Colocalization of transforming growth factor-α and a functional epidermal growth factor receptor (EGFR) to the inner cell mass and preferential localization of the EGFR on the basolateral surface of the trophectoderm in the mouse blastocyst. Dev Biol 154: 396–409.

    Google Scholar 

  • Dardik A, Schultz RM (1991) Blastocoel expansion in the preimplantation mouse embryo: stimulatory effect of TGF-α and EGF. Development 113: 919–30.

    Google Scholar 

  • Dealy CN, Scranton V, Cheng H-C (1998) Roles of transforming growth factor-α and epidermal growth factor in chick limb development. Dev Biol 202: 43–55.

    Google Scholar 

  • Duncun MD, Dorman LY, Bass BL (1994) Epidermal growth factor primes intestinal epithelial cells for proliferative effect of insulin-like growth factor I. Dig Dis Sci 39: 2197–201.

    Google Scholar 

  • Hashimoto K, Higashiyama S, Asada H, Hashimura E, Kobayashi T, Sudo K, Nakagawa T, Damm D, Yoshikawa K, Taniguchi N (1994) Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes. J Biol Chem 269: 20060–6.

    Google Scholar 

  • Hudson LG, McCawley LJ (1998) Contributions of the epidermal growth factor receptor to keratinocyte motility. Microsc Res Tech 43: 444–55.

    Google Scholar 

  • Johnson SE, Rothstein JL, Knowles BB (1994) Expression of epidermal growth factor family gene members in early mouse development. Dev Dynamics 201: 216–26.

    Google Scholar 

  • Kjelsberg C, Sakurai H, Spokes K, Birchmeier C, Drummond I, Higam S, Cantley LG (1997) Met-/-kidneys express epithelial cells that chemotax and form tubules in response to EGF receptor ligands. Am J Physiol 272: F222–8.

    Google Scholar 

  • Klein LM, DeWild LJ, McCarthy TA (1998) Effect of tyrosine kinase inhibition on surfactant protein A gene expression during human lung development. Am J Physiol 274: L542–51.

    Google Scholar 

  • Kirchen RV, West WR (1976) The Japanese medaka: its care and development. Carolina Biological Supply. Burlington: NC.

    Google Scholar 

  • Kornblum HI, Hussain RJ, Bronstein JM, Gall CM, Lee DC, Seroogy KB (1997) Prenatal ontogeny of the epidermal growth factor receptor and its ligand, transforming growth factor alpha, in the rat brain. J Comp Neurol 380: 243–61.

    Google Scholar 

  • Kumar Srivastava R, Van Der Kraak G (1995) Multifactorial regulation of DNA synthesis in goldfish ovarian follicles. Gen Comp Endocrinol 100: 397–403.

    Google Scholar 

  • Lillien L (1995) Changes in retinal cell fate induced by overexpression of EGF receptor. Nature 377: 158–62.

    Google Scholar 

  • Macdougall TM, Van Der Kraak G (1998) Peptide growth factors modulate prostaglandin E and F production by goldfish ovarian follicles. Gen Comp Endocrinol 110: 46–57.

    Google Scholar 

  • Martin KL, Barlow DH, Sargent IL (1998) Heparin-binding epidermal growth factor significantly improves human blastocyst development and hatching in serum-free medium. Hum Reprod 13: 1645–52.

    Google Scholar 

  • Menard D, Arsenault P, Pothier P (1988) Biologic effects of epidermal growth factor in human fetal jejunum. Gastroenterology 94: 656–63.

    Google Scholar 

  • Miettinen PJ, Berger JE, Meneses J, Phung Y, Pedersen RA, Werb Z, Derynck R(1995) Epithelial immaturity and multiorgan failure in mice lacking epidermal growth factor receptor. Nature 376: 337–41.

    Google Scholar 

  • Miettinen PJ, Warburton D, Bu D, Zhao J-S, Berger JE, Minoo P, Koivisto T, Allen L, Dobbs L, Werb Z, Derynck R(1997) Impaired lung branching morphogenesis in the absence of functional EGF receptor. Dev Biol 186: 224–36.

    Google Scholar 

  • Murillas R, Larcher F, Conti CJ, Santos M, Ullrich A, Jorcano JL (1995) Expression of a dominant negative mutant of epidermal growth factor receptor in the epidermis of transgenic mice elicits striking alterations in hair follicle development and skin structure. EMBO J 14: 5216–23.

    Google Scholar 

  • Paria BC, Das SK, Andrews GK, Dey SK (1993) Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation. Proc Natl Acad Sci USA 90: 55–9.

    Google Scholar 

  • Paria BC, Dey SK (1990) Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors. Proc Natl Acad Sci USA 87: 4756–60.

    Google Scholar 

  • Pati D, Balshaw K, Grinwich DL, Hollenberg MD, Habibi HR (1996) Epidermal growth factor receptor binding and biological activity in the ovary of goldfish, Carassius auratus. Am J Physiol 270: R1065–72.

    Google Scholar 

  • Peppelenbosch MP, Tertoolen LGJ, Hage WJ, De Laat SW (1993) Epidermal growth factor-induced actin remodeling is regulated by 5-lipoxygenase and cyclooxygenase products. Cell 74: 565–75.

    Google Scholar 

  • Perrimon N, Perkins LA (1997) There must be 50 ways to rule the signal: the case of the drosophila EGF receptor. Cell 89: 13–16.

    Google Scholar 

  • Polk DB, Tong W(1999) Epidermal and hepatocyte growth factors stimulate chemotaxis in an intestinal epithelial cell line. Am J Physiol 277: C1149–59.

    Google Scholar 

  • Rugh R (1962) Experimental embryology techniques and procedures, 3rd edition. Burgess Publishing Co., Minneapolis, MN.

    Google Scholar 

  • Schier A, Talbot WS (1998) The zebrafish organizer. Curr Opin Gen Dev 8: 464–71.

    Google Scholar 

  • Slowey MJ, Verhage HG, Fazleabas AT (1994) Epidermal growth factor, transforming growth factor-α, and epidermal growth factor receptor localization in the baboon (Papio anubis) uterus during the menstrual cycle and early pregnancy. J Soc Gynecol Invest 1: 277–84.

    Google Scholar 

  • Takashi I (1994) Stages of normal development in the medaka Orzias latipes. Zool Sci 11: 825–39.

    Google Scholar 

  • Tamada H, Higashiyama C, Takano H, Kawate N, Inaba T (1999) The effects of heparin-binding epidermal growth factor-like growth factor on preimplantation-embryo development and implantation in the rat. Life Sci 64: 1967–73.

    Google Scholar 

  • Tropepe V, Sibilia M, Ciruna BG, Rossant J, Wagner EF, Van DerKooy D (1999) Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol 208: 166–88.

    Google Scholar 

  • Van Zoelen EJJ, Stortelers C, Lenferink EG, Van De Poll MLM (2000) The EGF domain: requirements for binding to receptors of the erbB family. Vit Horm 59: 99–131.

    Google Scholar 

  • Vaughan TJ, James PS, Pascall JC, Brown KD (1992) Expression of the genes for TGFα, EGF and the EGF receptor during early pig development. Development 116: 663–9.

    Google Scholar 

  • Wiley LM, Adamson ED, Tsark EC (1995) Epidermal growth factor receptor function in early mammalian development. Bio Essays 17: 839–46.

    Google Scholar 

  • Wiley LM, Wu J-X, Harari I, Adamson ED (1992) Epidermal growth factor receptor mRNA and protein increase after the fourcell preimplantation stage in murine development. Dev Biol 149: 247–60.

    Google Scholar 

  • Wood SA, Kaye PL (1989) Effects of epidermal growth factor on preimplantation mouse embryos. J Reprod Fertil 85: 575–82.

    Google Scholar 

  • Zhang Y, Paria BC, Dey SK, Davis DL (1992) Characterization of the epidermal growth factor receptor in preimplantation pig conceptuses. Dev Biol 151: 617–21.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Trinity Christian College, Palos Heights, IL, 60463, USA

    Robert A. Boomsma, Heather Scott & Kendra Walters

Authors
  1. Robert A. Boomsma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Heather Scott
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kendra Walters
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boomsma, R.A., Scott, H. & Walters, K. Immunocytochemical Localization of Epidermal Growth Factor Receptor in Early Embryos of the Japanese Medaka Fish (Oryzias Latipes). Histochem J 33, 37–42 (2001). https://doi.org/10.1023/A:1017539829752

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017539829752

Keywords

Search

Navigation