Abstract
The effect of two α-amino acids, 2-amino-3-cyclopropyl-butanoicacid (ACPBA) and 2-amino-5-chloro-4-pentenoic acid (ACPA) on the growth oflettuce (Lactuca sativa L.) roots was investigated. BothACPBA and ACPA at 10−4 significantly inhibitedgrowth of roots of light-grown seedlings, while they had no effect on hypocotylgrowth. The lengths of cortical cells in the mature region of roots that hadbeen exposed to either compound were significantly shorter than those ofcontrolroots. Treatment with ACPBA and ACPA significantly decreased the mechanicalextensibility of root cell walls prior to the start of inhibition of rootgrowth. On the other hand, these compounds did not decrease the osmoticconcentration of the cell sap in root tissues. These results suggest that ACPBAand ACPA inhibit the cell elongation process of lettuce roots by decreasing themechanical extensibility of cell walls.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bacic A., Harris P.J. and Stone B.A. 1988. Structure and function of plant cell walls. In: Preiss J. (ed.), The Biochemistry of Plants. vol. 14 Academic Press, New York, pp. 297–371.
Büntemeyer K., Lüthen H. and Böttger M. 1998. Auxin-induced changes in cell wall extensibility of maize roots. Planta. 204: 515–519.
Cosgrove D.J. 1986. Biophysical control of plant cell growth. Annu. Rev. Plant. Physiol. 37: 377–405.
Darvill A., McNeil M., Albersheim P. and Delmer D.P. 1980. The primary cell walls of flowering plants. In: Stumpf P.K. and Conn E.E. (eds), The Biochemistry of Plants. vol. 1 Academic Press, New York, pp. 91–162.
Ohta T., Nakajima S., Sato Z., Aoki T., Hatanaka S. and Nozoe S. 1986. Cyclopropylalanine, an antifungal amino acid of the mushroom (Amanita vergineoides). Chemistry Letters.: 511–512.
Pritchard J., Wyn Jones R.G. and Tomos A.D. 1988. Control of wheat elongation growth. The effects of excision on growth, wall rheology and root anatomy. Planta. 176: 399–405.
Sakurai N. 1991. Cell wall function in growth and development. A physical and chemical point of view. Bot. Mag. Tokyo. 104: 235–251.
Taiz L. 1984. Plant cell expansion: regulation of cell wall mechanical properties. Annu. Rev. Plant Physiol. 35: 585–657.
Tanimoto E. and Huber D.J. 1997. Effect of GA3 on the molecular mass of polyuronides in cell walls of alaska pea roots. Plant Cell Physiol. 38: 25–35.
Thimann K.V. 1963. Plant growth substances; past, present and future. Annu. Rev. Plant Physiol. 14: 1–18.
Tsurumi S. and Wada S. 1995. Chromosaponin I stimulates the elongation of cortical cells in lettuce root. Plant Cell Physiol. 36: 925–929.
Tsurumi S., Watanabe R. and Kamisaka S. 1996. Chromosaponin I increases mechanical extensibility of lettuce root cell walls. Physiol. Plant. 97: 740–746.
Yamamoto R., Shinozaki K. and Masuda Y. 1970. Stress-relaxation properties of plant cell walls with special reference to auxin action. Plant Cell Physiol. 11: 947–956.
Yoshimura H., Takegami K., Doe M., Yamashita T., Shibata K., Wakabayashi K. et al. 1999. α-Amino acids from a mushroom, Amanita castanopsidis Hongo, with growth-inhibiting activity. Phytochemistry. 52: 25–27.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wakabayashi, K., Soga, K., Hoson, T. et al. Growth inhibition of lettuce (Lactuca sativa L.) roots by α-amino acids, 2-amino-3-cyclopropyl-butanoic acid and 2-amino-5-chloro-4-pentenoic acid, isolated from Amanita castanopsidis Hongo. Plant Growth Regulation 33, 169–173 (2001). https://doi.org/10.1023/A:1017527421294
Issue Date:
DOI: https://doi.org/10.1023/A:1017527421294