Abstract
The presence of digestive carbohydrases was determined in seabream intestines, and the main activity identified was amylase. Optimum activity for this enzyme was found at pH 8.0 at 40 °C. Amylase activity was highly sensitive to extreme pH, and temperatures exceeding 50 °C. The use of SDS–PAGE zymograms allowed identification of amylase in the form of a high molecular mass fraction exceeding 100 kDa. Results confirm the existence of a well developed amylase equipment in this species which supports the possibility of increasing the amount of carbohydrates in the formulation of its commercial feeds.
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Alarcón, F., Martínez, T., Díaz, M. et al. Characterization of digestive carbohydrase activity in the gilthead seabream (Sparus aurata). Hydrobiologia 445, 199–204 (2001). https://doi.org/10.1023/A:1017521900442
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DOI: https://doi.org/10.1023/A:1017521900442