Abstract
The high-molecular-weight (HMW) glutenin subunit composition of seed storage protein of 174 Japanese hexaploid wheat (Triticum aestivum) landraces have been examined by using sodium dodecyl sulfate polyacrylamide gel electrophoresis system. Twenty four different, major glutenin HMW subunits were identified, and each of the landraces contained three to five subunits and 17 different glutenin subunit patterns were observed for 13 alleles in the landraces. On the basis of HMW glutenin subunits composition, Japanese landraces showed a specific allelic variation, close to Japanese commercial wheats in HMW glutenin subunits, different from those in alien hexaploid wheats. Further, it could be concluded that all common glutenin alleles can be found in the 174 landraces originated from Japan. The variation detected in the glutenin subunits is useful for variety identification, has a bearing on our understanding of hexaploid wheat genetic resource evolution in Japan, and raises questions concerning the nature of this genetic variation.
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References
Asfaw, Z., 1989. Variation in hordein polypeptide pattern with Ethiopian barely, Hordeum vulgare L. Hereditas 110: 185–191.
Brown, A.H.D. & B.S. Weir, 1983. Measuring genetic variability in plant populations. In: Isozymes in Plant Genetics and Breeding, pp. 219–239, Part A, Elsevier, Amsterdam.
Ceccarelli, S., S. Grando & J.A.G. van Leur, 1987 Genetic diversity in barely landraces from Syria and Jordan. Euphytica 36: 389–405.
Gepts, P., 1989. Genetic diversity of seed storage proteins in plants. In: Plant Population Genetics, Breeding and Genetics Resources, pp. 64–82. Sinauer Associate Inc., Sundarland, Massachusetts.
Graybosch, R.A., C.J. Peterson, L.E. Hansen & P.J. Mattern, 1990. Relationships between protein solubility characteristics, 1BL/1RS, high molecular weight glutenin composition, and end-use quality in winter germplasm. Cereal Chem 67: 342–349.
Khan, K., G. Tamminga & O. Lukow, 1989. The effect of wheat flour proteins on mixing and baking-correlations with protein fractions and high molecular weight subunit composition by gel electrophoresis. Cereal Chem 66: 391–396.
Lawrence, G.J. & K.W. Shepherd, 1980. Variation in glutenin protein subunits of wheats. Aust J Bio Sci 33: 221–233.
Lawrence, G.J., 1986. The high-molecular-weight glutenin subunit composition of Australian wheat cultivars. Aust J Agric Res 37: 125–133.
Lukow, O.M., P.I. Payne & R. Tkachuk, 1989. The HMW glutenin subunit composition of Canadian wheat cultivars and their association with bread-making quality. J Sci Food Agric 46: 451–460.
McFadden, E.S. & E.R. Sears, 1946. The origin of triticum spelta and its free-threshing hexaploid relatives. J Hered 37: 81–89.
Miskelly, D.M., 1981. Quality requirements for manufacture of fresh and instant Chinese noodles. Proceedings 31st Annual Conference of the Royal Australian Chemical Institute Cereal Chem Div Perth 61–62.
Miskelly, D.M. & H.J. Moss, 1985. Flour quality requirements for Chinese noodle manufacture. J Cereal Sci 3: 379–387.
Morgunov, A.I., R.J. Pena, J. Crossa & S. Rajam, 1993. Worldwide distribution of Glu-1 alleles in bread wheat. J Genet & Breed 47: 53–60.
Nakamura, H., H. Sasaki, H. Hirano & A. Yamashita, 1990. A high molecular weight subunit of wheat glutenin seed storage protein correlates with its flour quality. Japan J Breed 40: 485–494.
Nakamura, H., A. Inazu & H. Hirano, 1999a. Allelic variation in high-molecular-weight glutenin subunit Loci of Glu-1 in Japanese common wheats. Euphytica 106: 131–138.
Nakamura, H., 1999b. Identification of alleles for complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for highmolecular-weight subunits of glutenin in Japanese hexaploid wheat varieties. J Agric Food Chem 47: 5273–5277.
Nakamura, H., 2000. The association between high-molecularweight glutenin subunit compositions and bread-making quality of Chinese and Japanese hexaploid wheats. Aust J Agric Res 51(3): 371–375.
Nevo, E., A. Beiles & D. Kaplan, 1988. Genetic diversity and environment association of wild emmer wheat in Turkey. Heredity 61: 31–45.
Ng, P.K.W. & W. Bushuk, 1989. Statistical relationships between high-molecular-weight subunits of glutenin and bread-making quality of Canadian-grown wheats. Cereal Chem 65: 408–413.
Payne, P.I., K.G. Corfield & J.A. Blackman, 1979. Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theor Appl Genet 55: 153–159.
Payne, P.I., L.M. Holt & C.N. Law, 1981. Structural and genetical studies on the high molecular weigh subunits of wheat glutenin. 1. Allelic variation in subunits amongst varieties of wheat. Theor Appl Genet 60: 229–236.
Payne, P.I & G.J. Lawrence, 1983. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11: 29–35.
Payne, P.I., L.M. Holt, E.A. Jackson & C.N. Law, 1984. Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Phil Trans R Soc Lond B304: 359–371.
Payne, P.I., M.A. Nightingale, A.F. Krattiger & L.M. Holt, 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric 40: 51–65.
Pogna, N.E., F. Mellini, A. Beretta & A. Dal Belin Peruffo, 1989. The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J Genet & Breed 43: 17–24.
Tolbert, D.M., C.O. Qualset, S.K. Jain & J.C. Craddock, 1979. A diversity analysis of a world collection of barely. Crop Sci 19: 789–794.
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Nakamura, H. Genetic diversity of high-molecular-weight glutenin subunit compositions in landraces of hexaploid wheat from Japan. Euphytica 120, 227–234 (2001). https://doi.org/10.1023/A:1017514423061
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DOI: https://doi.org/10.1023/A:1017514423061