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Induction of microspore embryos in a CMS line of Brassica juncea and formation of the androgenic plantlets

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Abstract

Anther culture of two wide hybrids (Diplotaxis erucoides × Brassica campestris) × B. juncea and (D. berthautii × B. campestris) × B. juncea, their CMS lines and the parent species elicited a range of responses highlighting the importance of the genotype. Androgenesis was expressed in cultured anthers of CMS (D. erucoides) B. juncea (22.8%), in restored pollen fertile plants of this CMS line (1.66%), and in the parent, B. juncea cv Pusa Bold (13.02%). AgNO3 was essential for androgenic response in the CMS lines, and it markedly increased the frequency of androgenesis in the cultivated species. Multiple crops of microspore embryos were obtained from responsive anthers of CMS plants in anther recultures. As high as92% microspore embryos of the CMS line germinated on basal B5medium and formed normal plantlets.

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References

  • Agarwal, P.K. & S.S. Bhojwani, 1993. Enhanced pollen grain embryogenesis and plant regeneration in anther cultures of Brassica juncea cv. PR-45. Euphytica 70: 191–196.

    Article  Google Scholar 

  • Bajaj, Y.P.S., 1983. In vitro production of haploids. In: D.A. Evans, W.R. Sharp, P.V. Ammirato & Y. Yamada (Eds.), Handbook of Plant Cell Culture, Vol I, pp. 228–287. Macmilan Publishing Co., New York.

    Google Scholar 

  • Bicar, E.H. & N.L. Darvey, 1997. Development of the components of a cytoplasmic male sterility hybrid system in rye through anther culture. Euphytica 97: 151–160.

    Article  Google Scholar 

  • Biddington, N.L., H.T. Robinson & J.R. Lynn, 1993. ABA promotion of ethylene production in anther culture of Brussels sprouts (Brassica oleracea var. gemmifera) and its relevance to embryogenesis. Physiol Plant 88: 577–582.

    Article  CAS  Google Scholar 

  • Choung, P.V., K.P. Pauls & W.D. Beversdorf, 1988. High frequency embryogenesis in male sterile plants of Brassica napus through microspore culture. Can J Bot 66: 1676–1680.

    Google Scholar 

  • Chunling, L., 1992. Agricultural Biotechnology. In: C.B. You & Z.C. Chen (Eds.), China Science and Technology Press, Beijing.

    Google Scholar 

  • De Buyser, J., Y. Henry, R. Lonnet, R. Hertzog & A. Hespel, 1987. 'Florin': A doubled haploid wheat variety developed by the anther culture method. Plant Breed 98: 53–56.

    Article  Google Scholar 

  • Foroughi-Wehr, B. & G. Wenzel, 1989. Androgenetic haploid production. IPTAC Newslett 58: 11–18.

    Google Scholar 

  • Gamborg, O.L., R.A. Miller & L. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158.

    Article  PubMed  CAS  Google Scholar 

  • George, L. & P.S. Rao, 1982. In vitro induction of pollen embryos and plantlets in Brassica juncea. Plant Sci Lett 26: 111–116.

    Article  Google Scholar 

  • Heberle-Bors, E., 1982. In vitro pollen embryogenesis in Nicotiana tabacum L. and its relation to pollen sterility, sex balance, and floral induction of the pollen donor plants. Planta 156: 396–401.

    Article  Google Scholar 

  • Heberle-Bors, E., 1985. In vitro haploid formation from pollen: a critical review. Theor Appl Genet 71: 361–374.

    Article  Google Scholar 

  • Heberle-Bors, E. & W. Odenbach, 1985. In vitro pollen embryogenesis and cytoplasmic male sterilty in Triticum aestivum. Z Pflanzenzoücht 95: 14–22.

    Google Scholar 

  • Heslop-Harrison, J. & Y. Heslop-Harrison, 1970. Evaluation of pollen viability by enzymatically induced fluorescence; intracelluler hydrolysis of fluorescein diacetate. Stain Tech 45: 115–120.

    CAS  Google Scholar 

  • Hu, H., T.Y. His, C.C. Tseng, T.W. Ouyang & C.K. Ching, 1978. Frontiers of plant tissue culture. In: T.A. Thorpe (Ed.), International Association of Plant Tissue Culture, Calgary.

    Google Scholar 

  • Huang, P.H., M.H. Lan & P.S. Chen, 1978. Selection and breeding of Ou-Hwa No.1 and No.2 and its performance. In: Proc Symp on Anther Culture, pp. 248–249. Science Press, Beijing (cmin Chinese).

    Google Scholar 

  • Katiyar, R.K., 1994. Embryogenesis in isolated microspore cultures of Indian mustard (Brassica juncea L.). Plant Tissue Cult 4: 117–121.

    Google Scholar 

  • Kaul, M.L.H., 1988. Male Sterility in Higher Plants. Springer-Verlag, Berlin.

    Google Scholar 

  • Keller, W.A., 1984. Anther culture of Brassica. In: I.K. Vasil (Ed.), Cell Culture and Somatic Cell Genetics of Plants, Vol 1, pp. 307–318. Academic Press, London.

    Google Scholar 

  • Keller, W.A. & K.C. Armstrong, 1977. Embryogenesis and plant regeneration in Brassica napus through anther cultures. Can J Bot 55: 1383–1388.

    Article  Google Scholar 

  • Keller, W.A., K.C. Armstrong & I.A. de la Roche, 1982. the production and utilization of microspore-derived haploids in Brassica crops. In: K.L. Giles & S.K. Sen (Eds.), Plant Cell Culture in Crop Improvement, pp. 169–183. Plenum Publ Co, New York.

    Google Scholar 

  • Keller, W.A., P.G. Arnison & B.K. Cardy, 1987. Haploids from gametophytic cells - recent developments and future prospects. In: C.E. Green, D.A. Somers, W.P. Hackett & D.D. Biesboer (Eds.), Plant Cell Tissue and Cell Culture, pp. 233–241. Allan R Liss, New York.

    Google Scholar 

  • Khush, G.S. & S.S. Virmani, 1996. Haploids in Plant Breeding. In: S.M. Jain, S.K. Sopory & R.E. Veilleux (Eds.), In Vitro Haploid Production in Higher Plants, Vol I, pp 11–33. Kluwer Academic Publ, Dordrecht, Boston, London.

    Google Scholar 

  • Maheshwari, S.C., A.K. Tyagi, K. Malhotra & S.K. Sopory, 1980. Induction of haploidy from pollen grains in angiosperms: the current status. Theor Appl Genet 58: 193–206.

    Google Scholar 

  • Malik, M., P. Vyas, N.S. Rangaswamy & K.R. Shivanna, 1999. Development of two new cytoplasmic male-sterile lines in Brassica juncea through wide hybridization. Plant Breed 118: 75–78.

    Article  Google Scholar 

  • Matzinger, D.F. & L.G. Burk, 1984. Cytoplasmic modification by anther culture in Nicotiana tabacum L. Journal of Heredity 75: 167–170.

    Google Scholar 

  • Nakamura, A., T. Yamada, M. Oka, Y. Tatemichi, K. Egushi, T. Ayabe & K. Kobayashi, 1975. Studies on the haploid method of breeding by anther culture in tobacco. V. Breeding of wild fluecured variety F211 by haploid method. Bull Iwata Tobacco Exp Station 7: 29–39.

    Google Scholar 

  • Nitsch, J.P., 1969. Experimental androgenesis in Nicotiana. Phytomorphology 19: 389–404.

    Google Scholar 

  • Palmer, C.E. & W.A. Keller, 1997. Pollen embryos. In: K.R. Shivanna & V.K. Sawney (Eds.), Pollen Biotechnology for Crop Production and Improvement, pp. 392–422. Cambridge University Press. 203

  • Palmer, C.E., W.A. Keller & P.G. Arnison, 1996a. Experimental haploidy in Brassica species. In: S.M. Jain, S.K. Sopory & R.E. Veilleux (Eds.), In Vitro Haploid Production in Higher Plants, Vol 3, pp. 143–172. Kluwer Academic Publ, Dordrecht, Boston, London.

    Google Scholar 

  • Palmer, C.E., W.A. Keller & P.G. Arnison, 1996b. Utilization of Brassica haploids. In: S.M. Jain, S.K. Sopory & R.E. Veilleux (Eds.), In Vitro Haploid Production in Higher Plants, Vol 3, pp. 173–192. Kluwer Academic Publ, Dordrecht, Boston, London.

    Google Scholar 

  • Prabhudesai, V. & S. Bhaskaran, 1993. A continuous culture system of direct somatic embryogenesis in microspore-derived embryos of Brassica juncea. Plant Cell Rep 12: 289–292.

    Article  Google Scholar 

  • Prakash, S., P.B. Kirti & V.L. Chopra, 1995. Cytoplasmic male sterility (CMS) systems other than Ogu and Polima in Brassica - Current status. Proc Internatl Rapeseed Cong Cambridge UK, pp. 44–48.

  • Rao, G.U., V. Batra-Sarup, S. Prakash & K.R. Shivanna, 1994. Development of a new cytoplasmic male sterile system in Brassica juncea through wide hybridization. Plant Breed 112: 171–174.

    Article  Google Scholar 

  • Rao, P.S. & P. Suprasanna, 1996. Methods to double haploid chromosome numbers. In: S.M. Jain, S.K. Sopory & R.E. Veilleux (Eds.), In Vitro Haploid Production in Higher Plants, Vol 1, pp. 317–339. Kluwer Academic Publ, Dordrecht, Boston, London.

    Google Scholar 

  • Sharma, K.K. & S.S. Bhojwani, 1985. Microspore embryogenesis in anther cultures of two Indian cultivars of Brassica juncea (L.) Czern. Plant Cell Tissue Organ Culture 4: 235–239.

    Article  Google Scholar 

  • Shen, L.Y. & R.E. Veilleux, 1995. Yield of embryos from sequential anther culture. American Potato Journal 72: 689–700.

    Google Scholar 

  • Sopory, S.K. & M. Munshi, 1996. Anther culture. In: S.M. Jain, edS.K. Sopory & R.E. Veilleux (Eds.), In Vitro Haploid Production in Higher Plants, Vol I, pp. 145–176. Kluwer Academic Publ, Dordrecht, Boston, London.

    Google Scholar 

  • Tsuchiya, K., 1993. Development of new rice varieties Hirohikari and Hirohonami, using haploid method of breeding by anther culture. In: K. Oono (Ed.), Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phytochemicals, pp. 191–196. Sato Press Mito, Japan.

    Google Scholar 

  • Vyas, P., S. Prakash & K.R. Shivanna, 1995. Production of wide hybrids and backcross progenies between Diplotaxis erucoides and crop brassicas. Theor Appl Genet 90: 549–553.

    Article  Google Scholar 

  • Yadav, R.C., N.R. Yadav, P.R. Kumar & D.R. Sharma, 1988. Differential androgenic response in Brassica juncea (L.) Czern and Coss. Cruciferae Newslett 13: 76.

    Google Scholar 

  • Zhensheng, L., 1992. Agricultural Biotechnology. In: C.B. You & Z.L. Chen (Eds.). China Science and Technology Press, Beijing.

    Google Scholar 

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Author notes

  1. N.S. Rangaswamy

    Present address: , KC-29B, Ashok Vihar I, Delhi, 110 052, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110 007, India

    Meghna Rana Malik, N.S. Rangaswamy & K.R. Shivanna

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  1. Meghna Rana Malik
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  2. N.S. Rangaswamy
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  3. K.R. Shivanna
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Malik, M.R., Rangaswamy, N. & Shivanna, K. Induction of microspore embryos in a CMS line of Brassica juncea and formation of the androgenic plantlets. Euphytica 120, 195–203 (2001). https://doi.org/10.1023/A:1017502113066

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