Skip to main content

Monthly changes in the content of fucans, their constituent sugars and sulphate in cultured Laminaria japonica

Abstract

Crude fucan was extracted monthly from makonbu, Laminaria japonica Areschoug, cultured from April to October at a southern site of the Hokkaido bay. The crude fucan yield tended to gradually increase from April to September, and rose markedly in October when spore formation was over. The fucans were fractionated on DEAE-Sephadex A-25 into three acidic components, A, B and C. In all the monthly extracts, fraction C was the largest. It was entirely free of laminaran and alginate, as was fraction B. Several sugars such as mannose and glucuronic acid, were identified in fraction C beside a large amount of fucose and sulphate. Only the proportion of the latter two constituents continued to increase as the alga matured to reach twice or even more than three times as much as all the other sugars in the September extract.

This is a preview of subscription content, access via your institution.

References

  • Abdel-Fattah, F. M. D., M. Hussein & H. M. Salem, 1974. Some structural features of sargassan, a sulphated heteropolysaccharide from Sargassum linifolium.Carbohydr. Res. 33: 19–24.

    Google Scholar 

  • Côté, R. H., 1959. Disaccharides from fucoidin. J. Chem. Soc.: 2248–2254.

  • Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers & F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.

    Google Scholar 

  • Fischer, F. G. & H. Dörfel, 1955. Die papierchromatographishe Trennung und Bestimmung der Uronsauren. Zeitschr. Physiol. Chem. 301: 224–234.

    Google Scholar 

  • Haug, A. & A. Jensen, 1956. Seasonal variation in chemical composition of Laminaria digitatafrom different parts of the Norwegian coast. Proc. int. Seaweed Symp. 2: 10–15.

    Google Scholar 

  • Haug, A. & B. Larsen, 1962. Quantitative determination of the uronic acid composition of alginates. Acta Chimica Scand. 16: 1908–1918.

    Google Scholar 

  • Honya, M., T. Kinoshita, M. Ishikawa, H. Mori & K. Nisizawa, 1993. Monthly determination of alginate, M / G ratio, mannitol and minerals in cultivated Laminaria japonica.Bull. Jap. Soc. Sci. Fish. (Nippon Suisan Gakkaishi) 59: 295–299.

    Google Scholar 

  • Kloareg, B., M. Demarty & M. Quillet, 1979. Extraction et purification du fucoidane de Pelvetia canaliculata(Dene et Thur.). Physiol. veg. 17: 731–747.

    Google Scholar 

  • Mabeau, S., B. Kloareg & J. P. Joseleau, 1990. Fractionation and analysis of fucans from brown algae. Phytochemistry 29: 2441–2445.

    Google Scholar 

  • Matsuhiro, B. & D. M. Zambrano, 1990. Carbohydrate constituents of Lessonia trabeculata.J. appl. Phycol. 2: 183–185.

    Google Scholar 

  • Medcalf, D. G., 1978. Sulfated fucose-containing polysaccharides from brown algae: Structural features and biochemical implications. Amer. chem. Soc. Symp. Series 77: 225–244.

    Google Scholar 

  • Mian, A. J. & E. Percival, 1973a. Carbohydrates of the brown seaweeds Himanthalia lorea, Bifurcaria bifurcataand Padina pavonia. Part I: Extraction and fractionation. Carbohydr. Res. 26: 133–146.

    Google Scholar 

  • Mian, A. J. & E. Percival, 1973b. Carbohydrates of the brown seaweeds Himanthalia loreaand Bifurcaria bifurcata.Part II: Structural studies of the ‘fucans’. Carbohydr. Res. 26: 147–161.

    Google Scholar 

  • Mori, H., H. Kamei, E. Nishide & K. Nisizawa, 1982. Sugar constituents of some sulfated polysaccharides from sporophylls of wakame (Undaria pinnatifida) and their biological activities. In Hoppe, H. A. & T. Levring (eds), Marine Algae in Pharmaceutical Science, Vol.2, Walter de Gruyter, Berlin/New York: 109–121.

    Google Scholar 

  • Mori, H. & K. Nisizawa, 1982. Sugar constituents of sulfated polysaccharides from the fronds of Sargassum ringgoldianum.Bull. Jap. Soc. Sci. Fish. ( Nippon Suisan Gakkaishi ) 48: 981–986.

    Google Scholar 

  • Ota, S., 1968. Microdetermination of sulfur in organic compounds with modified type combustion flask. Jap. Analyst 17: 1322–1324.

    Google Scholar 

  • Schöniger, W., 1955. Eine mikroanalytische Schnellbestimmung von Halogen in organischen Substanzen. Mikrochim. Acta 1: 123–129.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Honya, M., Mori, H., Anzai, M. et al. Monthly changes in the content of fucans, their constituent sugars and sulphate in cultured Laminaria japonica. Hydrobiologia 398, 411–416 (1999). https://doi.org/10.1023/A:1017007623005

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017007623005

  • brown algae
  • Laminaria japonica
  • fucan
  • sulphated polysaccharide
  • monthly change
  • molar ratio